LOCUS       sequence-182467-       10166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast vector for targeted deletion of MLO gene in barley 
            (CmYLCV promoter driving the gRNA array).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC437
FEATURES             Location/Qualifiers
     source          1..10166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     826..843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             3475..3531
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3532..7635
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3532..7635
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7636..7683
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      7694..7942
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     protein_bind    8439..8458
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        8479..8554
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8555..8574
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        8595..8670
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8671..8690
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     polyA_signal    8722..8896
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             9366..10157
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg gagagcgccc atatgcgcac tcctcgcatg cggcgcgccg atctgcaggc
      901 gcgcctgcag tgcagcgtga cccggtcgtg cccctctcta gagataatga gcattgcatg
      961 tctaagttat aaaaaattac cacatatttt ttttgtcaca cttgtttgaa gtgcagttta
     1021 tctatcttta tacatatatt taaactttac tctacgaata atataatcta tagtactaca
     1081 ataatatcag tgttttagag aatcatataa atgaacagtt agacatggtc taaaggacaa
     1141 ttgagtattt tgacaacagg actctacagt tttatctttt tagtgtgcat gtgttctcct
     1201 ttttttttgc aaatagcttc acctatataa tacttcatcc attttattag tacatccatt
     1261 tagggtttag ggttaatggt ttttatagac taattttttt agtacatcta ttttattcta
     1321 ttttagcctc taaattaaga aaactaaaac tctattttag tttttttatt taataattta
     1381 gatataaaat agaataaaat aaagtgacta aaaattaaac aaataccctt taagaaatta
     1441 aaaaaactaa ggaaacattt ttcttgtttc gagtagataa tgccagcctg ttaaacgccg
     1501 tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag
     1561 cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg
     1621 ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg
     1681 gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc
     1741 caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc
     1801 ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa
     1861 tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc ccctctctac
     1921 cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg
     1981 tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga
     2041 cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg
     2101 ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca
     2161 tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt
     2221 catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt
     2281 ctagatcgga gtagaattaa ttctgtttca aactacctgg tggatttatt aattttggat
     2341 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
     2401 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
     2461 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
     2521 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
     2581 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
     2641 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
     2701 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
     2761 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
     2821 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
     2881 ttggtgttac ttcctgcagg aagcatggac cactacctcg acatcaggct caggccagac
     2941 ccagagttcc caccagccca gctcatgtcc gtcctcttcg gcaagctcca ccaggccctc
     3001 gtggcccagg gcggcgacag gatcggcgtg tccttcccag acctcgacga gtccaggtcc
     3061 aggctcggcg agaggctccg catccacgcc tccgccgacg acctcagggc cctcctcgcc
     3121 aggccgtggc tggagggcct cagggaccac ctccagttcg gcgagccagc cgtggtgcca
     3181 cacccaaccc catacaggca agtgtccagg gtgcaagcca agtccaaccc agagaggctc
     3241 aggaggaggc tcatgaggag gcacgacctc tccgaggaag aggccaggaa gcgcatccca
     3301 gacaccgtgg ccagggccct cgacctccca ttcgtgaccc tcaggtccca gtccaccggc
     3361 cagcacttcc gcctcttcat caggcacggc ccactccagg tgaccgccga ggagggcggc
     3421 tttacctgct acggcctctc caagggcggc ttcgtgccgt ggttcggctc cggcgccacc
     3481 aacttctccc tcctcaagca agccggcgac gtggaggaga acccaggccc aatggacaag
     3541 aagtactcga tcggcctcga catcgggacg aactcagttg gctgggccgt gatcaccgac
     3601 gagtacaagg tgccctctaa gaagttcaag gtcctgggga acaccgaccg ccattccatc
     3661 aagaagaacc tcatcggcgc tctcctgttc gacagcgggg agaccgctga ggctacgagg
     3721 ctcaagagaa ccgctaggcg ccggtacacg agaaggaaga acaggatctg ctacctccaa
     3781 gagattttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag
     3841 tctttcctcg tggaggagga taagaagcac gagcggcatc ccatcttcgg caacatcgtg
     3901 gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg
     3961 gactccaccg ataaggcgga cctcagactg atctacctcg ctctggccca catgatcaag
     4021 ttccgcggcc atttcctgat cgagggggat ctcaacccag acaacagcga tgttgacaag
     4081 ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgcc
     4141 tctggcgtgg acgcgaaggc tatcctgtcc gcgaggctct cgaagtccag gaggctggag
     4201 aacctgatcg ctcagctccc aggcgagaag aagaacggcc tgttcgggaa cctcatcgct
     4261 ctcagcctgg ggctcacccc gaacttcaag tcgaacttcg atctcgctga ggacgccaag
     4321 ctgcaactct ccaaggacac ctacgacgat gacctcgata acctcctggc ccagatcggc
     4381 gatcaatacg cggacctgtt cctcgctgcc aagaacctgt cggacgccat cctcctgtca
     4441 gatatcctcc gcgtgaacac cgagatcacg aaggctccac tctctgcctc catgatcaag
     4501 cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc
     4561 ccggagaagt acaaggagat tttcttcgat cagtcgaaga acggctacgc tgggtacatc
     4621 gacggcgggg cctcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg
     4681 gacggcacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga
     4741 accttcgata acggcagcat cccccaccaa atccatctcg gggagctgca cgccatcctg
     4801 agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc
     4861 ctgaccttca gaatcccata ctacgtcggc cctctcgcgc gggggaactc aagattcgct
     4921 tggatgaccc gcaagtctga ggagaccatc acgccgtgga acttcgagga ggtggtggac
     4981 aagggcgcta gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc
     5041 aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag
     5101 ctcacgaagg tgaagtacgt caccgagggc atgcgcaagc cagcgttcct gtccggggag
     5161 cagaagaagg ctatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa
     5221 ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtcgagat cagcggcgtt
     5281 gaggaccgct tcaacgccag cctcgggacc taccacgatc tcctgaagat catcaaggat
     5341 aaggacttcc tggacaacga ggagaacgag gatatcctgg aggacatcgt gctgaccctc
     5401 acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc
     5461 gatgacaagg tcatgaagca actcaagcgc cggagataca ccggctgggg gaggctgtcc
     5521 cgcaagctca tcaacggcat ccgggacaag cagtccggga agaccatcct cgacttcctc
     5581 aagagcgatg gcttcgccaa caggaacttc atgcaactga tccacgatga cagcctcacc
     5641 ttcaaggagg atatccaaaa ggctcaagtg agcggccagg gggactcgct gcacgagcat
     5701 atcgcgaacc tcgctggctc ccccgcgatc aagaagggca tcctccagac cgtgaaggtt
     5761 gtggacgagc tcgtgaaggt catgggccgg cacaagcctg agaacatcgt catcgagatg
     5821 gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc
     5881 atcgaggagg gcatcaagga gctggggtcc caaatcctca aggagcaccc agtggagaac
     5941 acccaactgc agaacgagaa gctctacctg tactacctcc agaacggcag ggatatgtac
     6001 gtggaccaag agctggatat caaccgcctc agcgattacg acgtcgatca tatcgttccc
     6061 cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac
     6121 cgcggcaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg
     6181 aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct
     6241 gagagaggcg ggctctcaga gctggacaag gccggcttca tcaagcggca gctggtcgag
     6301 accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac
     6361 gatgagaacg acaagctgat cagggaggtt aaggtgatca ccctgaagtc taagctcgtc
     6421 tccgacttca ggaaggattt ccagttctac aaggttcgcg agatcaacaa ctaccaccat
     6481 gcccatgacg cttacctcaa cgctgtggtc ggcaccgctc tgatcaagaa gtacccaaag
     6541 ctggagtccg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc
     6601 aagtcggagc aagagatcgg caaggctacc gccaagtact tcttctactc aaacatcatg
     6661 aacttcttca agaccgagat cacgctggcc aacggcgaga tccggaagag accgctcatc
     6721 gagaccaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc
     6781 cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggc
     6841 gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag
     6901 aaggactggg acccaaagaa gtacggcggg ttcgacagcc ctaccgtggc ctactcggtc
     6961 ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctc
     7021 ctggggatca ccatcatgga gaggtccagc ttcgagaaga acccaatcga cttcctggag
     7081 gccaagggct acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc
     7141 ttcgagctgg agaacggcag gaagagaatg ctggcttccg ctggcgagct ccagaagggg
     7201 aacgagctcg cgctgccaag caagtacgtg aacttcctct acctggcttc ccactacgag
     7261 aagctcaagg gcagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag
     7321 cattacctcg acgagatcat cgagcaaatc tccgagttca gcaagcgcgt gatcctcgcc
     7381 gacgcgaacc tggataaggt cctctccgcc tacaacaagc accgggacaa gcccatcaga
     7441 gagcaagcgg agaacatcat ccatctcttc accctgacga acctcggcgc tcctgctgct
     7501 ttcaagtact tcgacaccac gatcgatcgg aagagataca cctccacgaa ggaggtcctg
     7561 gacgcgaccc tcatccacca gtcgatcacc ggcctgtacg agacgaggat cgacctctca
     7621 caactcggcg gggataagag acccgcagca accaagaagg cagggcaagc aaagaagaag
     7681 aagtgactcg agatatgaag atgaagatga aatatttggt gtgtcaaata aaaagcttgt
     7741 gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa
     7801 tattaaatga atgtaagatc acattataat gaataaacaa atgtttctat aatccattgt
     7861 gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact
     7921 atggaatatg attaaagata aggagctccg gtgacggacg gcgcgctggc agacatactg
     7981 tcccacaaat gaagatggaa tctgtaaaag aaaacgcgtg aaataatgcg tctgacaaag
     8041 gttaggtcgg ctgcctttaa tcaataccaa agtggtccct accacgatgg aaaaactgtg
     8101 cagtcggttt ggctttttct gacgaacaaa taagattcgt ggccgacagg tgggggtcca
     8161 ccatgtgaag gcatcttcag actccaataa tggagcaatg acgtaagggc ttacgaaata
     8221 agtaagggta gtttgggaaa tgtccactca cccgtcagtc tataaatact tagcccctcc
     8281 ctcattgtta agggagcaaa atctcagaga gatagtccta gagagagaaa gagagcaagt
     8341 agcctagaag tagtcaaggc ggcgaagtat tcaggcacgt ggccaggaag aagaaaagcc
     8401 aagacgacga aaacaggtaa gagctaagct tcctgcaggt tcactgccgt ataggcagga
     8461 cggacacgag caccatgggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     8521 ttatcaactt gaaaaagtgg caccgagtcg gtgcgttcac tgccgtatag gcagtaaatc
     8581 ctaacgacag gagggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     8641 caacttgaaa aagtggcacc gagtcggtgc gttcactgcc gtataggcag gtcagaagag
     8701 cagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg
     8761 aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     8821 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     8881 tactaaaatc cagatccccc gaattaccgg cgagctttgg gtacgtcacg tggctcgagc
     8941 gcgtagtcct cggtaatatc gcagaacaaa agtacctgat atcgagtgta cttcaagtca
     9001 caccggcgag tgccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     9061 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     9121 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     9181 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     9241 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     9301 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     9361 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     9421 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     9481 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     9541 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     9601 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     9661 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     9721 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     9781 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     9841 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     9901 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     9961 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
    10021 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
    10081 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
    10141 aggtagtcgg caaataaccc tcgagc
//
