LOCUS       sequence-18247-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Loftus SK, Larson DM, Watkins-Chow D, Church DM, Pavan WJ
  TITLE     Generation of RCAS vectors useful for functional genomic analyses.
  JOURNAL   DNA Res. 2001 Oct 31. 8(5):221-6.
  PUBMED    11759842
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res. 
            2001 Oct 31. 8(5):221-6."
COMMENT     Sequence Label: RCASBP-Y DV
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    94..218
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(243..248,249..266,267..273)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(393..412)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgtgaaatac gcttttgtct gtgtgctgca ggagctgagc tgactctgct ggtggcctcg
       61 cgtaccactg tggcatcggc ggccgcgttt atcacaagtt tgtacaaaaa agctgaacga
      121 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      181 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
      241 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
      301 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
      361 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
      421 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
      481 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
      541 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
      601 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccac
//
