LOCUS       sequence-182470-        9833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast vector expressing gRNA38 targeting barley MLO (with TREX2
            expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 9833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC436
FEATURES             Location/Qualifiers
     source          1..9833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     826..843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             3624..3680
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3681..7784
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3681..7784
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7785..7832
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      7843..8091
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        8491..8566
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             9033..9824
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg gagagcgccc atatgcgcac tcctcgcatg cggcgcgccg atctgcaggc
      901 gcgcctgcag tgcagcgtga cccggtcgtg cccctctcta gagataatga gcattgcatg
      961 tctaagttat aaaaaattac cacatatttt ttttgtcaca cttgtttgaa gtgcagttta
     1021 tctatcttta tacatatatt taaactttac tctacgaata atataatcta tagtactaca
     1081 ataatatcag tgttttagag aatcatataa atgaacagtt agacatggtc taaaggacaa
     1141 ttgagtattt tgacaacagg actctacagt tttatctttt tagtgtgcat gtgttctcct
     1201 ttttttttgc aaatagcttc acctatataa tacttcatcc attttattag tacatccatt
     1261 tagggtttag ggttaatggt ttttatagac taattttttt agtacatcta ttttattcta
     1321 ttttagcctc taaattaaga aaactaaaac tctattttag tttttttatt taataattta
     1381 gatataaaat agaataaaat aaagtgacta aaaattaaac aaataccctt taagaaatta
     1441 aaaaaactaa ggaaacattt ttcttgtttc gagtagataa tgccagcctg ttaaacgccg
     1501 tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag
     1561 cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg
     1621 ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg
     1681 gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc
     1741 caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc
     1801 ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa
     1861 tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc ccccctctct
     1921 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1981 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     2041 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     2101 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     2161 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     2221 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     2281 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     2341 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     2401 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     2461 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     2521 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     2581 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     2641 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     2701 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     2761 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     2821 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     2881 gtttggtgtt acttcctgca ggaagcatgt ctgagccacc tcgggctgag acctttgtat
     2941 tcctggacct agaagccact gggctcccaa acatggaccc tgagattgca gagatatccc
     3001 tttttgctgt tcaccgctct tccctggaga acccagaacg ggatgattct ggttccttgg
     3061 tgctgccccg tgttctggac aagctcacac tgtgcatgtg cccggagcgc ccctttactg
     3121 ccaaggccag tgagattact ggtttgagca gcgaaagcct gatgcactgc gggaaggctg
     3181 gtttcaatgg cgctgtggta aggacactgc agggcttcct aagccgccag gagggcccca
     3241 tctgccttgt ggcccacaat ggcttcgatt atgacttccc actgctgtgc acggagctac
     3301 aacgtctggg tgcccatctg ccccaagaca ctgtctgcct ggacacactg cctgcattgc
     3361 ggggcctgga ccgtgctcac agccacggca ccagggctca aggccgcaaa agctacagcc
     3421 tggccagtct cttccaccgc tacttccagg ctgaacccag tgctgcccat tcagcagaag
     3481 gtgatgtgca caccctgctt ctgatcttcc tgcatcgtgc tcctgagctg ctcgcctggg
     3541 cagatgagca ggcccgcagc tgggctcata ttgagcccat gtacgtgcca cctgatggtc
     3601 caagcctcga agccggctcc ggcgccacca acttctccct cctcaagcaa gccggcgacg
     3661 tggaggagaa cccaggccca atggacaaga agtactcgat cggcctcgac atcgggacga
     3721 actcagttgg ctgggccgtg atcaccgacg agtacaaggt gccctctaag aagttcaagg
     3781 tcctggggaa caccgaccgc cattccatca agaagaacct catcggcgct ctcctgttcg
     3841 acagcgggga gaccgctgag gctacgaggc tcaagagaac cgctaggcgc cggtacacga
     3901 gaaggaagaa caggatctgc tacctccaag agattttctc caacgagatg gccaaggttg
     3961 acgattcatt cttccaccgc ctggaggagt ctttcctcgt ggaggaggat aagaagcacg
     4021 agcggcatcc catcttcggc aacatcgtgg acgaggttgc ctaccacgag aagtacccta
     4081 cgatctacca tctgcggaag aagctcgtgg actccaccga taaggcggac ctcagactga
     4141 tctacctcgc tctggcccac atgatcaagt tccgcggcca tttcctgatc gagggggatc
     4201 tcaacccaga caacagcgat gttgacaagc tgttcatcca actcgtgcag acctacaacc
     4261 aactcttcga ggagaacccg atcaacgcct ctggcgtgga cgcgaaggct atcctgtccg
     4321 cgaggctctc gaagtccagg aggctggaga acctgatcgc tcagctccca ggcgagaaga
     4381 agaacggcct gttcgggaac ctcatcgctc tcagcctggg gctcaccccg aacttcaagt
     4441 cgaacttcga tctcgctgag gacgccaagc tgcaactctc caaggacacc tacgacgatg
     4501 acctcgataa cctcctggcc cagatcggcg atcaatacgc ggacctgttc ctcgctgcca
     4561 agaacctgtc ggacgccatc ctcctgtcag atatcctccg cgtgaacacc gagatcacga
     4621 aggctccact ctctgcctcc atgatcaagc gctacgacga gcaccatcag gatctgaccc
     4681 tcctgaaggc gctggtccgc caacagctcc cggagaagta caaggagatt ttcttcgatc
     4741 agtcgaagaa cggctacgct gggtacatcg acggcggggc ctcacaagag gagttctaca
     4801 agttcatcaa gccaatcctg gagaagatgg acggcacgga ggagctcctg gtgaagctca
     4861 acagggagga cctcctgcgg aagcagagaa ccttcgataa cggcagcatc ccccaccaaa
     4921 tccatctcgg ggagctgcac gccatcctga gaaggcaaga ggacttctac cctttcctca
     4981 aggataaccg ggagaagatc gagaagatcc tgaccttcag aatcccatac tacgtcggcc
     5041 ctctcgcgcg ggggaactca agattcgctt ggatgacccg caagtctgag gagaccatca
     5101 cgccgtggaa cttcgaggag gtggtggaca agggcgctag cgctcagtcg ttcatcgaga
     5161 ggatgaccaa cttcgacaag aacctgccca acgagaaggt gctccctaag cactcgctcc
     5221 tgtacgagta cttcaccgtc tacaacgagc tcacgaaggt gaagtacgtc accgagggca
     5281 tgcgcaagcc agcgttcctg tccggggagc agaagaaggc tatcgtggac ctcctgttca
     5341 agaccaaccg gaaggtcacg gttaagcaac tcaaggagga ctacttcaag aagatcgagt
     5401 gcttcgattc ggtcgagatc agcggcgttg aggaccgctt caacgccagc ctcgggacct
     5461 accacgatct cctgaagatc atcaaggata aggacttcct ggacaacgag gagaacgagg
     5521 atatcctgga ggacatcgtg ctgaccctca cgctgttcga ggacagggag atgatcgagg
     5581 agcgcctgaa gacgtacgcc catctcttcg atgacaaggt catgaagcaa ctcaagcgcc
     5641 ggagatacac cggctggggg aggctgtccc gcaagctcat caacggcatc cgggacaagc
     5701 agtccgggaa gaccatcctc gacttcctca agagcgatgg cttcgccaac aggaacttca
     5761 tgcaactgat ccacgatgac agcctcacct tcaaggagga tatccaaaag gctcaagtga
     5821 gcggccaggg ggactcgctg cacgagcata tcgcgaacct cgctggctcc cccgcgatca
     5881 agaagggcat cctccagacc gtgaaggttg tggacgagct cgtgaaggtc atgggccggc
     5941 acaagcctga gaacatcgtc atcgagatgg ccagagagaa ccaaaccacg cagaaggggc
     6001 aaaagaactc tagggagcgc atgaagcgca tcgaggaggg catcaaggag ctggggtccc
     6061 aaatcctcaa ggagcaccca gtggagaaca cccaactgca gaacgagaag ctctacctgt
     6121 actacctcca gaacggcagg gatatgtacg tggaccaaga gctggatatc aaccgcctca
     6181 gcgattacga cgtcgatcat atcgttcccc agtctttcct gaaggatgac tccatcgaca
     6241 acaaggtcct caccaggtcg gacaagaacc gcggcaagtc agataacgtt ccatctgagg
     6301 aggtcgttaa gaagatgaag aactactgga ggcagctcct gaacgccaag ctgatcacgc
     6361 aaaggaagtt cgacaacctc accaaggctg agagaggcgg gctctcagag ctggacaagg
     6421 ccggcttcat caagcggcag ctggtcgaga ccagacaaat cacgaagcac gttgcgcaaa
     6481 tcctcgactc tcggatgaac acgaagtacg atgagaacga caagctgatc agggaggtta
     6541 aggtgatcac cctgaagtct aagctcgtct ccgacttcag gaaggatttc cagttctaca
     6601 aggttcgcga gatcaacaac taccaccatg cccatgacgc ttacctcaac gctgtggtcg
     6661 gcaccgctct gatcaagaag tacccaaagc tggagtccga gttcgtgtac ggggactaca
     6721 aggtttacga tgtgcgcaag atgatcgcca agtcggagca agagatcggc aaggctaccg
     6781 ccaagtactt cttctactca aacatcatga acttcttcaa gaccgagatc acgctggcca
     6841 acggcgagat ccggaagaga ccgctcatcg agaccaacgg cgagacgggg gagatcgtgt
     6901 gggacaaggg cagggatttc gcgaccgtcc gcaaggttct ctccatgccc caggtgaaca
     6961 tcgtcaagaa gaccgaggtc caaacgggcg ggttctcaaa ggagtctatc ctgcctaagc
     7021 ggaacagcga caagctcatc gccagaaaga aggactggga cccaaagaag tacggcgggt
     7081 tcgacagccc taccgtggcc tactcggtcc tggttgtggc gaaggttgag aagggcaagt
     7141 ccaagaagct caagagcgtg aaggagctcc tggggatcac catcatggag aggtccagct
     7201 tcgagaagaa cccaatcgac ttcctggagg ccaagggcta caaggaggtg aagaaggacc
     7261 tgatcatcaa gctcccgaag tactctctct tcgagctgga gaacggcagg aagagaatgc
     7321 tggcttccgc tggcgagctc cagaagggga acgagctcgc gctgccaagc aagtacgtga
     7381 acttcctcta cctggcttcc cactacgaga agctcaaggg cagcccggag gacaacgagc
     7441 aaaagcagct gttcgtcgag cagcacaagc attacctcga cgagatcatc gagcaaatct
     7501 ccgagttcag caagcgcgtg atcctcgccg acgcgaacct ggataaggtc ctctccgcct
     7561 acaacaagca ccgggacaag cccatcagag agcaagcgga gaacatcatc catctcttca
     7621 ccctgacgaa cctcggcgct cctgctgctt tcaagtactt cgacaccacg atcgatcgga
     7681 agagatacac ctccacgaag gaggtcctgg acgcgaccct catccaccag tcgatcaccg
     7741 gcctgtacga gacgaggatc gacctctcac aactcggcgg ggataagaga cccgcagcaa
     7801 ccaagaaggc agggcaagca aagaagaaga agtgactcga gatatgaaga tgaagatgaa
     7861 atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg
     7921 ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagatca cattataatg
     7981 aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt ctgcagcata
     8041 taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa ggagctccgg
     8101 tgacggacga ccaagcccgt tattctgaca gttctggtgc tcaacacatt tatatttatc
     8161 aaggagcaca ttgttactca ctgctaggag ggaatcgaac taggaatatt gatcagagga
     8221 actacgagag agctgaagat aactgccctc tagctctcac tgatctgggt cgcatagtga
     8281 gatgcagccc acgtgagttc agcaacggtc tagcgctggg cttttaggcc cgcatgatcg
     8341 ggcttttgtc gggtggtcga cgtgttcacg attggggaga gcaacgcagc agttcctctt
     8401 agtttagtcc cacctcgcct gtccagcaga gttctgaccg gtttataaac tcgcttgctg
     8461 catcagactt gacggacacg agcaccatgg gttttagagc tagaaatagc aagttaaaat
     8521 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttccggcga
     8581 gctttgggta cgtcacgtgg ctcgagcgcg tagtcctcgg taatatcgca gaacaaaagt
     8641 acctgatatc gagtgtactt caagtcacac cggcgagtgc cagccaggac agaaatgcct
     8701 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     8761 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     8821 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     8881 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     8941 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     9001 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     9061 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     9121 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     9181 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     9241 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     9301 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     9361 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     9421 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     9481 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     9541 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     9601 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     9661 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     9721 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     9781 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agc
//
