LOCUS       sequence-182472-        7995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast vector expressing gRNA24 targeting tomato ANT1 (control 
            without TREX2 expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC394
FEATURES             Location/Qualifiers
     source          1..7995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     826..843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1389..1734
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1677..1697
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1768..5871
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1768..5871
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5884..5904
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5915..6163
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        6652..6727
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             7195..7986
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg gagagcgccc atatgcgcac tcctcgcatg cggcgcgccg atcggcgcgc
      901 cagatttgcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      961 gcagcaggta tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
     1021 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
     1081 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1141 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1201 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1261 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1321 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1381 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1441 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1501 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1561 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1621 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1681 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1741 actcctgcag gtagatcgct cgtcgacatg gataagaagt actctatcgg actcgatatc
     1801 ggaactaact ctgtgggatg ggctgtgatc accgatgagt acaaggtgcc atctaagaag
     1861 ttcaaggttc tcggaaacac cgataggcac tctatcaaga aaaaccttat cggtgctctc
     1921 ctcttcgatt ctggtgaaac tgctgaggct accagactca agagaaccgc tagaagaagg
     1981 tacaccagaa gaaagaacag gatctgctac ctccaagaga tcttctctaa cgagatggct
     2041 aaagtggatg attcattctt ccacaggctc gaagagtcat tcctcgtgga agaagataag
     2101 aagcacgaga ggcaccctat cttcggaaac atcgttgatg aggtggcata ccacgagaag
     2161 taccctacta tctaccacct cagaaagaag ctcgttgatt ctactgataa ggctgatctc
     2221 aggctcatct acctcgctct cgctcacatg atcaagttca gaggacactt cctcatcgag
     2281 ggtgatctca accctgataa ctctgatgtg gataagttgt tcatccagct cgtgcagacc
     2341 tacaaccagc ttttcgaaga gaaccctatc aacgcttcag gtgtggatgc taaggctatc
     2401 ctctctgcta ggctctctaa gtcaagaagg cttgagaacc tcattgctca gctccctggt
     2461 gagaagaaga acggactttt cggaaacttg atcgctctct ctctcggact cacccctaac
     2521 ttcaagtcta acttcgatct cgctgaggat gcaaagctcc agctctcaaa ggatacctac
     2581 gatgatgatc tcgataacct cctcgctcag atcggagatc agtacgctga tttgttcctc
     2641 gctgctaaga acctctctga tgctatcctc ctcagtgata tcctcagagt gaacaccgag
     2701 atcaccaagg ctccactctc agcttctatg atcaagagat acgatgagca ccaccaggat
     2761 ctcacacttc tcaaggctct tgttagacag cagctcccag agaagtacaa agagattttc
     2821 ttcgatcagt ctaagaacgg atacgctggt tacatcgatg gtggtgcatc tcaagaagag
     2881 ttctacaagt tcatcaagcc tatcctcgag aagatggatg gaaccgagga actcctcgtg
     2941 aagctcaata gagaggatct tctcagaaag cagaggacct tcgataacgg atctatccct
     3001 catcagatcc acctcggaga gttgcacgct atccttagaa ggcaagagga tttctaccca
     3061 ttcctcaagg ataacaggga aaagattgag aagattctca ccttcagaat cccttactac
     3121 gtgggacctc tcgctagagg aaactcaaga ttcgcttgga tgaccagaaa gtctgaggaa
     3181 accatcaccc cttggaactt cgaagaggtg gtggataagg gtgctagtgc tcagtctttc
     3241 atcgagagga tgaccaactt cgataagaac cttccaaacg agaaggtgct ccctaagcac
     3301 tctttgctct acgagtactt caccgtgtac aacgagttga ccaaggttaa gtacgtgacc
     3361 gagggaatga ggaagcctgc ttttttgtca ggtgagcaaa agaaggctat cgttgatctc
     3421 ttgttcaaga ccaacagaaa ggtgaccgtg aagcagctca aagaggatta cttcaagaaa
     3481 atcgagtgct tcgattcagt tgagatttct ggtgttgagg ataggttcaa cgcatctctc
     3541 ggaacctacc acgatctcct caagatcatt aaggataagg atttcttgga taacgaggaa
     3601 aacgaggata tcttggagga tatcgttctt accctcaccc tctttgaaga tagagagatg
     3661 attgaagaaa ggctcaagac ctacgctcat ctcttcgatg ataaggtgat gaagcagttg
     3721 aagagaagaa gatacactgg ttggggaagg ctctcaagaa agctcattaa cggaatcagg
     3781 gataagcagt ctggaaagac aatccttgat ttcctcaagt ctgatggatt cgctaacaga
     3841 aacttcatgc agctcatcca cgatgattct ctcaccttta aagaggatat ccagaaggct
     3901 caggtttcag gacagggtga tagtctccat gagcatatcg ctaacctcgc tggatctcct
     3961 gcaatcaaga agggaatcct ccagactgtg aaggttgtgg atgagttggt gaaggtgatg
     4021 ggaaggcata agcctgagaa catcgtgatc gaaatggcta gagagaacca gaccactcag
     4081 aagggacaga agaactctag ggaaaggatg aagaggatcg aggaaggtat caaagagctt
     4141 ggatctcaga tcctcaaaga gcaccctgtt gagaacactc agctccagaa tgagaagctc
     4201 tacctctact acctccagaa cggaagggat atgtatgtgg atcaagagtt ggatatcaac
     4261 aggctctctg attacgatgt tgatcatatc gtgccacagt cattcttgaa ggatgattct
     4321 atcgataaca aggtgctcac caggtctgat aagaacaggg gtaagagtga taacgtgcca
     4381 agtgaagagg ttgtgaagaa aatgaagaac tattggaggc agctcctcaa cgctaagctc
     4441 atcactcaga gaaagttcga taacttgact aaggctgaga ggggaggact ctctgaattg
     4501 gataaggcag gattcatcaa gaggcagctt gtggaaacca ggcagatcac taagcacgtt
     4561 gcacagatcc tcgattctag gatgaacacc aagtacgatg agaacgataa gttgatcagg
     4621 gaagtgaagg ttatcaccct caagtcaaag ctcgtgtctg atttcagaaa ggatttccaa
     4681 ttctacaagg tgagggaaat caacaactac caccacgctc acgatgctta ccttaacgct
     4741 gttgttggaa ccgctctcat caagaagtat cctaagctcg agtcagagtt cgtgtacggt
     4801 gattacaagg tgtacgatgt gaggaagatg atcgctaagt ctgagcaaga gatcggaaag
     4861 gctaccgcta agtatttctt ctactctaac atcatgaatt tcttcaagac cgagattacc
     4921 ctcgctaacg gtgagatcag aaagaggcca ctcatcgaga caaacggtga aacaggtgag
     4981 atcgtgtggg ataagggaag ggatttcgct accgttagaa aggtgctctc tatgccacag
     5041 gtgaacatcg ttaagaaaac cgaggtgcag accggtggat tctctaaaga gtctatcctc
     5101 cctaagagga actctgataa gctcattgct aggaagaagg attgggaccc taagaaatac
     5161 ggtggtttcg attctcctac cgtggcttac tctgttctcg ttgtggctaa ggttgagaag
     5221 ggaaagagta agaagctcaa gtctgttaag gaacttctcg gaatcactat catggaaagg
     5281 tcatctttcg agaagaaccc aatcgatttc ctcgaggcta agggatacaa agaggttaag
     5341 aaggatctca tcatcaagct cccaaagtac tcactcttcg aactcgagaa cggtagaaag
     5401 aggatgctcg cttctgctgg tgagcttcaa aagggaaacg agcttgctct cccatctaag
     5461 tacgttaact ttctttacct cgcttctcac tacgagaagt tgaagggatc tccagaagat
     5521 aacgagcaga agcaactttt cgttgagcag cacaagcact acttggatga gatcatcgag
     5581 cagatctctg agttctctaa aagggtgatc ctcgctgatg caaacctcga taaggtgttg
     5641 tctgcttaca acaagcacag agataagcct atcagggaac aggcagagaa catcatccat
     5701 ctcttcaccc ttaccaacct cggtgctcct gctgctttca agtacttcga tacaaccatc
     5761 gataggaaga gatacacctc taccaaagaa gtgctcgatg ctaccctcat ccatcagtct
     5821 atcactggac tctacgagac taggatcgat ctctcacagc tcggtggtga ttcaagggct
     5881 gatcctaaga agaagaggaa ggtttgactc gagatatgaa gatgaagatg aaatatttgg
     5941 tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta
     6001 tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca
     6061 aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact
     6121 gtatgtgcta tggtatggac tatggaatat gattaaagat aaggagctcc ggtgacggac
     6181 ccatggcttc gttgaacaac ggaaactcga cttgccttcc gcacaataca tcatttcttc
     6241 ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag cttacatttt
     6301 cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt ttgtatcttg
     6361 tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga atttgattga
     6421 ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct gggaatctga
     6481 aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta tataggccca
     6541 tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa cgaagctgag
     6601 tttatataca gctagagtcg aagtagtgat tggacgagga ggaacattgc agttttagag
     6661 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     6721 tcggtgcttt ttttcccggc gagctttggg tacgtcacgt ggctcgagcg cgtagtcctc
     6781 ggtaatatcg cagaacaaaa gtacctgata tcgagtgtac ttcaagtcac accggcgagt
     6841 gccagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca
     6901 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     6961 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     7021 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     7081 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     7141 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
     7201 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     7261 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     7321 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     7381 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     7441 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     7501 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     7561 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     7621 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     7681 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     7741 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     7801 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     7861 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     7921 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     7981 aaataaccct cgagc
//
