LOCUS       sequence-182473-        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Stx3 locked into the open conformation with a C-term
            myc-myc-his tag in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Giovannone AJ, Reales E, Bhattaram P, Fraile-Ramos A, Weimbs T
  TITLE     Monoubiquitination of syntaxin 3 leads to retrieval from the 
            basolateral plasma membrane and facilitates cargo recruitment to 
            exosomes.
  JOURNAL   Mol Biol Cell. 2017 Oct 15;28(21):2843-2853. doi: 
            10.1091/mbc.E17-07-0461. Epub 2017 Aug 16.
  PUBMED    28814500
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-07-0461"; journalName: "Mol Biol Cell"; date: 
            "2017-10-15- 15"; volume: "28"; issue: "21"; pages: "2843-2853"
COMMENT     Sequence Label: pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     68..92
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             815..826
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     gap             1112..1122
                     /estimated_length=11
ORIGIN
        1 gnnnnnnnag cngagctctc cctatcngtg atagagatct ccctatcagt gatagagatc
       61 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      121 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc
      181 ttggtaccga gctcggatcc actagtccag tgtggtggaa ttcctgggct tcaggatgaa
      241 ggaccgtctg gagcagctga aggccaagca gctgacacag gatgatgata ctgatgcggt
      301 tgagattgct atcgacaaca cggcttttat ggacgagttc ttttctgaga ttgaggaaac
      361 tcggcttaac attgacaaga tctcagaaca tgtagaggag gctaagaaac tctacagtat
      421 cattctctct gcaccgattc cagagccaaa aaccaaggat gacctagagc agctcacgac
      481 tgagattaag aaaagggcca acaacgtccg gaacaaactg aagagcatgg agaagcatat
      541 tgaagaagat gaggtcaggt catcggcaga ccttcggatt cggaaatccc agcactctgt
      601 cctttctcgg aagtttgtgg aggtgatgac caaatacaat gaagctcaag tggacttccg
      661 agaacgcagc aaagggcgaa tccagcggca gctcgaaatt actggcaaaa agacaaccga
      721 tgaggaggcg gcggagatgt tggagagtgg caacccggcc atcttcactt ctgggatcat
      781 tgactcacag atttccaagc aagccctcag tgagattgag ggacgacaca aggacnttgt
      841 gaggctggag agcagcatca aggagcttca cgacatgntt atggacatcg ccatgctggt
      901 gganaatcag ggtgagatgt taganaacat agagttgaat gtcatgcaca cagtgnnnca
      961 cgtggnnann nacgagatga aacgaaaaag ctgtgaanta ccagagtcng gcccnnanaa
     1021 atnatnatnt cntnngctag nannnnnnnn tngnnnttta ncatngatnn ntngnntttn
     1081 cnnngnngaa ngnnnnnncn nnnannaaan cnnnnnnnnn nnanangnnn ngg
//
