LOCUS       sequence-182477-       14462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant
            Selection: PvUbi2:hpt II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14462)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 14462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_25K
FEATURES             Location/Qualifiers
     source          1..14462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(2876..3552)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2919..2939)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5729..5794
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5801..5821
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6260..6565)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(6362..6381)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             7639..7695
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7699..7764
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7771..7791
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8119..8526)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     8376..8398
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8390..8407
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8391..8407
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8417..8436
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8417..8435
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8483..8503)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8483..8501)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8522..8538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8522..8538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8535..8557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8546..8562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8570..8576,8577..8594,8595..8600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8615..8636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             8822..9409
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      9423..9671
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    9692..9716
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11017..11646
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12075..13148
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13214..13408
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13644..13666)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13752..13892
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13807..13826
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13907..13924)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(14078..14462,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14158..14177)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaaatag agcttgtcga
     1801 tcgttaatta atcacctcca agatcccgga cgagtgctgg ggcgtcggtt tccactatcg
     1861 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
     1921 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
     1981 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
     2041 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
     2101 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     2161 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
     2221 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
     2281 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
     2341 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
     2401 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
     2461 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg
     2521 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
     2581 ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
     2641 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
     2701 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
     2761 atcaggtcgg acacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
     2821 agttcaggct ttttcatcct aggagagata gatttgtaga gagagactgg tgatttcagc
     2881 gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
     2941 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
     3001 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     3061 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     3121 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     3181 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     3241 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     3301 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     3361 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
     3421 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
     3481 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
     3541 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
     3601 acgagagtgt cgtgctccac catgggcgcg cctgcagtgc agcgtgaccc ggtcgtgccc
     3661 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     3721 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     3781 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     3841 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     3901 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     3961 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     4021 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     4081 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     4141 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     4201 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     4261 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     4321 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     4381 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     4441 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
     4501 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     4561 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     4621 cgctcgtcct cccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta
     4681 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg
     4741 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact
     4801 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg
     4861 atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca
     4921 atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt
     4981 gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattctgt ttcaaactac
     5041 ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca tagttacgaa
     5101 ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat gcgggtttta
     5161 ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg tgtggttggg
     5221 cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct ggtgtattta
     5281 ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt taagatggat
     5341 ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat gcatatacat
     5401 gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct attataataa
     5461 acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca tatgcagcag
     5521 ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg gtactgtttc
     5581 ttttgtcgat gctcaccctg ttgtttggtg ttacttcctg cagggaattc caagcaacga
     5641 actgcgagtg attcaagaaa aaagaaaacc tgagctttcg atctcttcgg agtggtttct
     5701 tgttctttga aaaagagggg gaagcatgga ttataaggat cacgatggcg actacaagga
     5761 ccacgatatt gactacaaag acgatgacga taaaatggct cctaagaaaa agcgcaaagt
     5821 cggtatccat ggcgttccct ctagaatggt ggatctacgc acgctcggct acagtcagca
     5881 gcagcaagag aagatcaaac cgaaggtgcg ttcgacagtg gcgcagcacc acgaggcact
     5941 ggtgggccat gggtttacac acgcgcacat cgttgcgctc agccaacacc cggcagcgtt
     6001 agggaccgtc gctgtcacgt atcagcacat aatcacggcg ttgccagagg cgacacacga
     6061 agacatcgtt ggcgtcggca aacagtggtc cggcgcacgc gccctggagg ccttgctcac
     6121 ggatgcgggg gagttgagag gtccgccgtt acagttggac acaggccaac ttgtgaagat
     6181 tgcaaaacgt ggcggcgtga ccgcaatgga ggcagtgcat gccagcagga atgcgttaac
     6241 aggtgccccc ctggagacgt tatattcccc agaacatcag gttaatggcg tttttgatgt
     6301 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggaaacc ggcacactgg
     6361 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     6421 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     6481 cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     6541 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
     6601 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     6661 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
     6721 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     6781 gcttcatagc atacctcctt ttgacatact tcgggtatac atatcagtat atattcttat
     6841 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttcgtctc caacgaccac
     6901 ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa aaagggattg
     6961 ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg cacgtcccat
     7021 cgcgttgccg gatcccagct ggtgaagtcc gagctggaag aaaaaaagag cgagctgcgc
     7081 cacaagctca agtacgtgcc ccacgagtac atcgagctga tcgagatcgc ccgcaacagc
     7141 acccaagacc gcatcctgga gatgaaagtg atggagttct tcatgaaggt gtacggctac
     7201 cgcggcaagc acctgggcgg ctcccgcaag cccgatggcg ccatctacac cgtgggctcc
     7261 cccatcgact atggcgtcat tgtcgacacc aaggcctact ccggcggcta caacttaccc
     7321 atcggtcagg ccgacgagat gcaacgctac gtgaaggaga accagacccg caataagcac
     7381 attaatccca acgagtggtg gaaggtgtac ccctcctccg tgaccgagtt caaattcctg
     7441 ttcgtgtccg gccacttcaa gggcaattat aaggcccaac tgacccgcct gaaccgcaag
     7501 accaactgca acggcgccgt gctgtccgtg gaggaactgc tgatcggcgg cgagatgatc
     7561 aaggctggta ccctgaccct ggaagaggtg cgccgcaagt tcaacaatgg tgaaatcaat
     7621 ttcaggtccg gaagcggcgc cacgaacttc tctctgttaa agcaagcagg tgacgtggaa
     7681 gaaaacccag ggcccatgga ctacaaagac catgacggtg attataaaga tcatgacatc
     7741 gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt gggcattcac
     7801 ggggtgccgg ctagcatggt ggatctacgc acgctcggct acagtcagca gcagcaagag
     7861 aagatcaaac cgaaggtgcg ttcgacagtg gcgcagcacc acgaggcact ggtgggccat
     7921 gggtttacac acgcgcacat cgttgcgctc agccaacacc cggcagcgtt agggaccgtc
     7981 gctgtcacgt atcagcacat aatcacggcg ttgccagagg cgacacacga agacatcgtt
     8041 ggcgtcggca aacagtggtc cggcgcacgc gccctggagg ccttgctcac ggatgcgggg
     8101 gagttgagag gtccgccgtt acagttggac acaggccaac ttgtgaagat tgcaaaacgt
     8161 ggcggcgtga ccgcaatgga ggcagtgcat gcatcgcgca atgcactgac gggtgccccc
     8221 ctggagaccg gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     8281 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     8341 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     8401 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccgcccct
     8461 tgaggtcctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     8521 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8581 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8641 cgttgcgctc actgcccgct ttccaccggt ggtctccaac gatcatctgg tggcactcgc
     8701 gtgcctcggc ggacgtcctg ccatggatgc agtgaaaaag ggattgccgc acgcgccgga
     8761 attgatcaga agagtcaatc gccgtattgg cgaacgcacg tcccatcgcg ttgccagatc
     8821 tcaactagtc aaaagtgaac tggaggagaa gaaatctgaa cttcgtcata aattgaaata
     8881 tgtgcctcat gaatatattg aattaattga aattgccaga aattccactc aggatagaat
     8941 tcttgaaatg aaggtaatgg aattttttat gaaagtttat ggatatagag gtaaacattt
     9001 gggtggatca aggaaaccgg acggagcaat ttatactgtc ggatctccta ttgattacgg
     9061 tgtgatcgtg gatactaaag cttatagcgg aggttataat ctgccaattg gccaagcaga
     9121 tgaaatggag cgatatgtcg aagaaaatca aacacgagac aaacatctca accctaatga
     9181 atggtggaaa gtctatccat cttctgtaac ggaatttaag tttttatttg tgagtggtca
     9241 ctttaaagga aactacaaag ctcagcttac acgattaaat catatcacta attgtaatgg
     9301 agctgttctt agtgtagaag agcttttaat tggtggagaa atgattaaag ccggcacatt
     9361 aaccttagag gaagtgaggc ggaaatttaa taacggcgag ataaactttt aatagctcga
     9421 gatatgaaga tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt
     9481 tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc tttttctaat attaaatgaa
     9541 tgtaagatca cattataatg aataaacaaa tgtttctata atccattgtg aatgttttgt
     9601 tggatctctt ctgcagcata taactactgt atgtgctatg gtatggacta tggaatatga
     9661 ttaaagataa ggagctcaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     9721 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     9781 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
     9841 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     9901 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
     9961 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
    10021 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
    10081 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
    10141 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
    10201 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
    10261 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
    10321 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
    10381 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
    10441 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
    10501 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
    10561 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
    10621 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
    10681 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
    10741 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
    10801 cgagccgccc gcgcacggct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
    10861 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
    10921 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
    10981 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
    11041 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
    11101 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
    11161 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
    11221 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
    11281 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
    11341 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
    11401 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
    11461 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
    11521 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
    11581 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
    11641 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
    11701 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
    11761 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
    11821 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
    11881 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
    11941 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
    12001 tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag
    12061 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
    12121 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
    12181 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
    12241 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
    12301 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
    12361 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
    12421 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
    12481 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
    12541 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
    12601 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
    12661 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
    12721 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
    12781 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
    12841 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
    12901 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
    12961 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
    13021 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
    13081 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
    13141 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
    13201 aaggcctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
    13261 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
    13321 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
    13381 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
    13441 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
    13501 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
    13561 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
    13621 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggaaac ggtcacagct
    13681 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    13741 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
    13801 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    13861 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
    13921 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    13981 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    14041 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    14101 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    14161 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    14221 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    14281 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    14341 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    14401 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    14461 at
//
