LOCUS       sequence-18248-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Loftus SK, Larson DM, Watkins-Chow D, Church DM, Pavan WJ
  TITLE     Generation of RCAS vectors useful for functional genomic analyses.
  JOURNAL   DNA Res. 2001 Oct 31. 8(5):221-6.
  PUBMED    11759842
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res. 
            2001 Oct 31. 8(5):221-6."
COMMENT     Sequence Label: RCASBP-Y NHA
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    143..267
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(292..297,298..315,316..322)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(442..461)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gctattgggt attgtgaata cgcttttgtc tgtgtgctgc aggagctgag ctgactctgc
       61 tggtggcctc gcgtaccact gtggcatcgg cggccgcacc atggcctacc cttatgacgt
      121 gccagattat gcctgtttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      181 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      241 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      301 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      361 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      421 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      481 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      541 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      601 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      661 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      721 tttctacaca tatatt
//
