LOCUS       sequence-18249-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Loftus SK, Larson DM, Watkins-Chow D, Church DM, Pavan WJ
  TITLE     Generation of RCAS vectors useful for functional genomic analyses.
  JOURNAL   DNA Res. 2001 Oct 31. 8(5):221-6.
  PUBMED    11759842
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res. 
            2001 Oct 31. 8(5):221-6."
COMMENT     Sequence Label: RCASBP-Y CHA
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    104..228
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(253..258,259..276,277..283)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(403..422)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gctattggta ttgtgaatac gcttttgtct gtgtgctgca ggagctgagc tgactctgct
       61 ggtggcctcg cgtaccactg tggcatcggc ggccgcgttt atcacaagtt tgtacaaaaa
      121 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
      181 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
      241 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
      301 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
      361 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
      421 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
      481 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
      541 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
      601 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
      661 tacca
//
