LOCUS       sequence-182495-        3193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP in the presence of E. coli sigma factor ?28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3193)
  TITLE     Unpublished Murray Lab Plasmids
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTar-deGFP
FEATURES             Location/Qualifiers
     source          1..3193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             294..316
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(355..376)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(616..635)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     963..984
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     rep_origin      complement(1360..1948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1440..1459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2119..2910,2911..2979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHATRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2742..2761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2980..3084)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3152..3170
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgaatcctct gaccagccag aaaacgacct ttctgtggtg aaaccggatg ctgcaattca
       61 gagcgccagc aagtggggga cagcagatga ccttaccgcc gcagagtgga tgtttgacat
      121 ggtgatgact atcgcaccat cagccagaaa accgaatttt gctgggtggg ctaacgatat
      181 ccgcctgatg cgtgaacgtg acggacgtaa cgaagacaac cacgcattgc tgttccaagc
      241 ttcaataaag tttcccccct ccttgccgat aacgagatca agctagcaat aattttgttt
      301 aactttaaga aggagatata cccaatggag cttttcactg gcgttgttcc catcctggtc
      361 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      421 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      481 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      541 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      601 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      661 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      721 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      781 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      841 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      901 gacaaccact acctgagcac acagtccgcc ctgagcaaag accacaacga gaagcgcgat
      961 cacatggtcc tgctggagtt cgtgaccgcc gccgggatct aatgaactaa cgcatgagaa
     1021 agcccccgga agatcacctt ccgggggctt ttttatgcgc ccttgagagt cgggcattgt
     1081 cttcgctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtgttc
     1141 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactta
     1201 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1261 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1321 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1381 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1441 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1501 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1561 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1621 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1681 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1741 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1801 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1861 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1921 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1981 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2041 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2101 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2161 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2221 gggcttacca tctggcccca gtgctgcaat gataccgcgt gacccacgct caccggctcc
     2281 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2341 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2401 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2461 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagttg catgatcccc
     2521 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2581 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2641 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2701 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     2761 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2821 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2881 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2941 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3001 ttgaagcatt tatcagggtt attgtctcat tagcggatac atatttgaat gtatttagaa
     3061 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     3121 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtgt
     3181 tcaagaattc tgg
//
