LOCUS       sequence-182534-       16589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Cook, Jean
  TITLE     Huwe1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR1A-Huwe1
FEATURES             Location/Qualifiers
     source          1..16589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(14461..14560)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(14672..14691)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             14683..15492
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(14750..14769)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      15582..16170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16071..16090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      16335..16421
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      16513..16540
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     16548..16571
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ttaaaggaac caattcagtc
      121 gacatgaaag tagacaggac taaactgaag aagacaccta ctgaggctcc tgcagactgc
      181 agagccttaa tagacaaact caaagtttgt aatgatgagc aacttctctt ggaactgcag
      241 cagatcaaaa catggaacat tggaaagtgc gagttatatc actgggtgga cctgttggac
      301 cgcttcgatg gaatactggc agatgctgga cagacagtgg agaatatgtc atggatgctc
      361 gtatgtgata ggccagaaag agagcaactg aaaatgcttc tcttggctgt gttgaacttc
      421 acagccttgc tcattgagta cagcttttcc cggcatctgt acagttccat agagcatttg
      481 acaactttat tggcttcctc tgatatgcaa gtggtgctgg cagtcctcaa tctcctatat
      541 gtatttagca aaagatcaaa ctacatcact cgtctgggat ctgacaagag gaccccgctg
      601 ctaactcggc tacaacattt ggcagagagc tggggtggaa aggagaatgg ctttggactt
      661 gcagaatgtt gcagagactt gcatatgatg aaatatccac ccagtgcaac tacactacac
      721 tttgaattct atgcagatcc tggggccgag gtcaaaattg agaaaaggac aactagtaac
      781 acactacatt atattcacat agagcaactt gacaagattt cagaaagccc ttctgaaatc
      841 atggaatctc ttaccaaaat gtacagcatt cctaaggata agcagatgct gttatttaca
      901 cacatacgac tggcccatgg cttttctaat cacaggaagc gattgcaggc agttcaggcc
      961 agactgcatg caatatctat attagtgtat tccaatgcct tgcaggaatc agcaaacagt
     1021 atcttgtata atggcttgat agaggagttg gtagatgtcc ttcagataac ggataagcag
     1081 cttatggaga ttaaagcagc ttctttacga acattaacat caattgtcca cttggagaga
     1141 actcccaaac tcagcagtat tattgactgt actggaactg cctcctacca tggatttttg
     1201 ccagtgcttg taaggaactg tatccaggcc atgattgatc cttccatgga tccataccct
     1261 caccagtttg ccactgctct cttctctttt ttataccatc tggccagcta cgatgctggt
     1321 ggtgaagcct tggtctcctg tggaatgatg gaagccttat tgaaggtcat aaagtttctt
     1381 ggcgatgaac aggaccagat aacatttgtc accagagccg tcagagtggt tgaccttatc
     1441 accaacctgg atatggcagc ttttcaatcc catagtggac tttctatctt catttataga
     1501 cttgagcatg aagtagatct gtgccgaaaa gaatgtccgt ttgtgatcaa gccaaagatc
     1561 cagagaccca atactacaca agaaggagag gaaatggaaa ctgatatgga tggagtccag
     1621 tgtattccac aacgagcagc acttctgaaa tccatgttga atttcctcaa gaaggccatc
     1681 caagaccctg ctttctcaga tggcatacga catgtgatgg atggttctct gcctacctcc
     1741 ctgaaacaca tcatcagcaa tgcagaatac tatggcccat cactcttcct cctagctact
     1801 gaagtggtga ctgtgtttgt atttcaagaa ccatcactgc tctcctcact ccaggacaat
     1861 ggattgacag atgtcatgct gcatgcactg cttatcaaag atgttcctgc tacccgtgaa
     1921 gtccttggct ccctcccaaa tgtattcagt gcactctgtt tgaatgcccg aggtcttcag
     1981 tcttttgttc agtgtcagcc ttttgaacgc ctcttcaaag ttcttctgtc tccagattac
     2041 ctcccagcca tgcggaggag gagaagttct gatccccttg gggatactgc atccaacctg
     2101 gggagtgctg tcgatgagct catgagacat cagcccaccc ttaaaacaga tgcaacgact
     2161 gccatcatca agttacttga agaaatctgt aatcttggaa gggaccccaa atacatctgt
     2221 cagaagccat cgatccagaa ggcagatggc actgccactg ctcctccccc aaggtctaat
     2281 catgccgcag aagaagcctc tagtgaggat gaggaggaag aggaagtaca ggccatgcag
     2341 agctttaatt ctacccagca aaatgaaact gagcctaatc agcaggttgt tggtacagag
     2401 gaacgtattc ctattcccct catggattac atccttaatg tgatgaaatt tgtggaatct
     2461 attctgagca acaatacaac agatgaccac tgccaggaat ttgtgaatca gaaaggactg
     2521 ttgcctttgg ttaccatttt gggtcttccc aatctgccca ttgactttcc cacatctgct
     2581 gcctgtcagg ctgttgcagg tgtctgcaaa tccatattga cactgtcaca tgaacccaaa
     2641 gtccttcaag agggtctcct tcagttggac tccatcctct cctccctgga gcccttacac
     2701 cgccccattg aatcccctgg gggctcagtg ttgttgcgag aactggcttg cgcaggcaat
     2761 gttgctgatg ctaccctctc agcccaggcc acacctctgc tgcatgcact cactgctgcc
     2821 catgcctaca tcatgatgtt tgttcatact tgcagagttg gacagagtga aattcgttcc
     2881 atctccgtaa accagtgggg ctctcaattg ggtctgagtg ttttgagcaa gctgagccag
     2941 ttatactgtt ccctggtgtg ggaaagcact gtcctcctct ctctgtgtac cccaaacagc
     3001 ctaccatctg ggtgtgaatt tggccaggca gatatgcaga aactggttcc aaaggatgag
     3061 aaggcaggta cgacccaggg cggaaaaaga tcagatgggg aacaggatgg agcagctgga
     3121 agtatggatg cttctaccca gggcttatta gaaggcattg ggctagatgg tgacacattg
     3181 gctcccatgg agacagatga acctactgct tcagactcta agggcaaatc taaaatcaca
     3241 ccagcaatgg ctgccagaat taagcaaatc aagcctttgt tatcagcttc ctccagatta
     3301 ggccgagcac ttgctgagct atttggactt cttgttaaac tttgtgtggg atctcctgtc
     3361 cgccagagaa ggagccatca tgctgccagc accactacag caccgacacc tgccgcgcga
     3421 tcaacagcct cagctctcac taagctcttg actaaggggt tatcttggca gcccccacca
     3481 tatacaccta ctccccgatt caggctgaca ttcttcatct gttcagttgg tttcacatcc
     3541 ccaatgctgt ttgatgagag gaagtatccc taccacctca tgctgcaaaa atttctctgc
     3601 tccggaggcc acaatgctct ttttgaaact ttcaactggg ctctgtccat gggaggtaaa
     3661 gttcctgttt ctgagggatt ggaacactca gacttgcctg atggcacagg agaattccta
     3721 gatgcctggc ttatgctggt ggagaagatg gtgaatccca ccacggtgct tgaatctcca
     3781 cattcgctgc ctgccaaatt gcctggaggt gtccagaact ttccccagtt cagtgcactg
     3841 cgcttccttg tggtaactca gaaagcagcc tttacttgca tcaaaaactt atggaaccgg
     3901 aaacccctga aggtatatgg tggacgaatg gctgaatcga tgctggccat tctatgccac
     3961 atcctccgag gagaacctgt gattcgagag agactaagca aggagaagga ggggtctcga
     4021 ggagaagagg atacagggca agaggaaggt ggctcccgcc gggaacctca agtcaaccag
     4081 caacaactgc aacagctcat ggacatgggc ttcacaaggg aacatgcaat ggaggcactg
     4141 ttgaacacca gcaccatgga gcaggccaca gagtaccttt taacccaccc tcctccaatc
     4201 atgggaggag ttgttcggga tctcagcatg tctgaagagg accagatgat gagagcaatt
     4261 gctatgtctc tgggacagga tattccaatg gatcaaaggg cagagtcacc tgaggaagtt
     4321 gcttgccgga aggaggaaga ggaacggaaa gctcgggaaa agcaggagga ggaagaggct
     4381 aaatgtctag agaagttcca ggatgctgac ccgttggaac aagatgagct ccacactttc
     4441 acagatacta tgttgccagg ctgcttccac cttcttgatg agctgccaga cacagtatac
     4501 cgtgtgtgtg acctgatcat gacagcaatc aaacgtaatg gagcagatta tcgtgacatg
     4561 attctgaagc aagtagtcaa tcaggtgtgg gaagctgctg atgtattgat caaagctgct
     4621 cttcccctga caacaagtga cacaaaaacc gtgtcagagt ggataagtca gatggccaca
     4681 ctgccccagg cctccaattt ggctactaga atcttgcttt taacgctact ttttgaggag
     4741 ttgaagctac cttgtgcttg ggtggttgaa tcaagtggca tccttaatgt cctaatcaaa
     4801 ctcttggaag tggttcagcc ctgcctccag gcagccaagg agcagaagga agtccagacc
     4861 ccaaagtgga tcacaccagt gttgctcctg attgatttct atgaaaagac agccatctcc
     4921 tcaaaaagga gagcccagat gactaagtac ctgcaatcca acagcaacaa ctggcgctgg
     4981 tttgatgatc gctctgggcg ttggtgtagt tacagtgcaa gcaacaatag cactattgat
     5041 tctgcctgga aatctggaga gacaagcgtg cgattcactg caggccgaag aagatacacg
     5101 gtccaattca ctacaatggt gcaggttaat gaggaaacag ggaaccgacg ccctgtgatg
     5161 ctgactctcc tcagggtacc tcggctgaat aaaaattcaa aaaacagcaa tggacaggaa
     5221 ctagagaaga cgctggaaga aagcaaagaa atggatatca aacgtaaaga aaataaaggc
     5281 aatgataccc ctttggccct agagagtaca aacactgaaa aggagacaag cctggaggaa
     5341 acaaaaatcg gggagatcct gatccagggc ttgacagaag atatggtgac tgttttaatc
     5401 cgggcctgcg tgagcatgct gggagtccct gtggacccag atactttgca tgccaccctt
     5461 cgtctctgtc tgaggctcac ccgggaccac aaatatgcca tgatgtttgc agaactgaag
     5521 agtacccgca tgatcttgaa tttgacccag agctcaggct tcaatgggtt tactcccctg
     5581 gtcacccttc tcttaagaca catcattgag gacccctgta cccttcgtca taccatggaa
     5641 aaggttgttc gctcagcagc tacaagtgga gctggtagca ctacctctgg tgttgtgtct
     5701 ggcagcctcg gctctcggga gatcaactac atccttcgtg tccttgggcc agccgcatgc
     5761 cgcaatccag acatattcac agaagtggcc aactgctgta tccgcatcgc ccttcctgcc
     5821 cctcgaggct caggaactgc ttcagatgat gaatttgaga atcttagaat taaaggccct
     5881 aatgctgtac agctggtgaa gaccacccct ttgaagccct cacctctgcc tgtcatccct
     5941 gatactatca aggaagtgat ctatgatatg ctgaatgctc tggctgcata ccatgctcca
     6001 gaggaagata aatctgatcc taaacctggg gttatgaccc aagaggttgg ccagctcctg
     6061 caagacatgg gtgatgatgt ataccagcag taccggtcac ttacgcgtca gagcagtgac
     6121 tttgatacgc agtcaggttt ttccattaat agtcaggtct ttgctgcaga tggtgcctcc
     6181 actgagactt ccgcatctgg gacctcccaa ggagaggctt caactccaga ggagtctcga
     6241 gatgggaaga aagataaaga aggggaccgg gcctctgagg aaggcaaaca gaaaggcaag
     6301 ggcagcaaac ctttaatgcc tacctccact atccttcgtc ttctggcaga gttggtgagg
     6361 tcctatgttg gtattgctac cctgattgcc aactacagct acactgtggg ccagtctgaa
     6421 ctgatcaaag aggactgcag tgtgctagct tttgttctgg accacctgct cccacatacc
     6481 cagaatgcag aagacaagga cacccctgcc ttggcccgcc tgttcctcgc aagcctggct
     6541 gctgcaggga gtggcacaga tgcccaggtg gccctagtga atgaagtaaa agcagccctt
     6601 ggacgggcac tggctatggc tgagagtaca gagaaacatg ccaggcttca ggcagtgatg
     6661 tgtatcatca gtactatcat ggagtcctgc ccctccacct ccagcttcta cagcagtgcc
     6721 acagcgaaga cccagcacaa tggcatgaac aacatcattc ggcttttcct gaagaaggga
     6781 ctggttaatg acctggccag agtacctcac agcttagacc tgtccagtcc caacatggcc
     6841 aacacagtca atgctgctct gaagcctttg gaaacacttt cccggattgt gaaccagccc
     6901 agtagccttt ttggcagcaa gagtgcttct agcaagaaca agtctgagca ggatgcccaa
     6961 ggagcctctc aagattccag tagcaaccag caggacccag gcgagcctgg ggaagcagaa
     7021 gtgcaggagg aggatcatga tgtcactcag acagaggtgg cagatgggga tatcatggat
     7081 ggggaggctg aaaccgactc agtggtgatt gctgggcagc ctgaggtgct cagttcacaa
     7141 gagatgcagg ttgagaatga gctggaggac ctgatagatg agttgcttga gagggatggc
     7201 ggatctggga acagtacaat tatagtgagc agaagtggag aggatgaatc acaagaggac
     7261 gtgctgatgg atgaagctcc ttccaacctc agccaagctt ccaccttgca ggccaaccga
     7321 gaagattcca tgaatatcct ggaccctgag gatgaggagg agcacactca ggaagaggac
     7381 agcagtggca gtaacgagga tgaggatgat agtcaggatg aagaggagga ggaggaggaa
     7441 gatgaggaag atgatcagga ggatgatgaa ggtgaagagg gagatgaaga cgatgacgac
     7501 gatggctctg agatggaatt ggatgaggat tatcctgata tgaacgcttc tcccttggtc
     7561 cgatttgagc gctttgaccg ggaggatgat ctcatcattg agtttgacaa catgttctcc
     7621 agtgctacag acatcccccc atccccagga aatatcccta ccacccatcc actgatggtg
     7681 cgccatgcag accacagttc tctgacactg ggcagtggct cttcaacaac tcgtctcacc
     7741 cagggcatcg ggcgcagtca gaggacccta aggcagctga cggccaatac tggccacacc
     7801 attcatgttc actaccctgg gaatcgccag cccaaccctc ctcttatact gcagaggttg
     7861 cttggtccct cagctgctgc tgacatcctt cagctgagca gcagccttcc cctacaaagc
     7921 cggggtcggg cccgcctcct ggtaggcaac gatgacgtcc acatcatcgc ccgttctgat
     7981 gatgagctgc tggatgactt tttccatgat cagagcacag ctaccagcca agcaggaacc
     8041 ctgtccagca tccccacagc cctgacccgc tggacagaag aatgcaaagt tctcgatgct
     8101 gagagcatgc atgactgtgt ttcagtggtt aaagtgtcca ttgtcaatca cctggaattc
     8161 ctgagggatg aggagctgga agaaaggcga gagaagcgca ggaaacaact ggctgaggaa
     8221 gaaacaaaga taactgataa aggcaaagaa gataaggaga acagggatca gagtgcacag
     8281 tgtactgcat ctaagtcaaa tgactccact gaacagaatc tctcagatgg gacgcctatg
     8341 cctgacagct acccaacaac cccatcttca actgatgcag ctacatctga gtccaaggag
     8401 acccttggca ctctgcaatc ctcacaacag caaccaacac tcccaacccc accagctttg
     8461 ggagaggttc ctcaggagct gcagtctcca gctggagaag ggggcagctc tacacagcta
     8521 ttgatgcctg tagagccaga ggaattgggt cccacaaggc caagtgggga agcagaaaca
     8581 actcagatgg agttatcccc agctcccact ataacctcac tttccccaga gagagctgag
     8641 gattctgatg cactgacggc tgtcagcagt cagctagaag gctctcctat ggatacaagc
     8701 agcctggctt cctgtacctt agaggaggct gtgggtgaca cttcagcagc tggcagttct
     8761 gagcagccca gagcaggcag ctccactcct ggggatgccc caccagctgt ggcggaagtg
     8821 caaggcagga gtgatgggtc aggggaatct gcccagccac ctgaggacag ctccccacct
     8881 gcatcctctg agagctcttc caccagagat tctgccgtgg ccatttctgg agcagattcc
     8941 cgaggaatcc tagaagagcc gttgccttca acaagcagtg aagaagaaga tccccttgcg
     9001 ggtatcagtc tccctgaagg tgtggacccc tcttttctgg ctgccctgcc tgatgacatc
     9061 cgtcgggaag ttctacagaa ccagctaggc attcgtccac caacccggac tgccccctcc
     9121 acaaatagct cagcgcctgc agtggtgggg aatcctggtg tgactgaagt actggcacag
     9181 cagagagctg agcagcagcg acgagaacta gcacagaatg ccagctcaga cacccctatg
     9241 gaccctgtga ccttcatcca gactctgccc tcagacctgc gccgtagtgt cctagaggat
     9301 atggaggaca gtgtgttagc tgtgatgcca cctgacattg cagctgaggc tcaagccctg
     9361 agacgagagc aagaagcccg gcagcgacag ctcatgcatg agcgtctgtt tgggcacagt
     9421 agcacctccg cactctctgc tattctccga agcccggctt tcaccagtcg cttaagtggc
     9481 aaccgtgggg tccagtatac tcgccttgct gtgcagagag gtggcacctt ccagatgggg
     9541 ggtagcagca gccataacag gccttctggc agtaatgtag atactctcct ccgcctccga
     9601 ggacggctcc ttctggacca cgaagccctt tcttgtctct tggtcctact ttttgtggat
     9661 gagccaaagc tcaatactag ccgtctacac cgagtactga gaaatctctg ctaccatgcc
     9721 cagacccgcc actgggtcat ccgcagtctg ctctccatct tgcagcgcag cagtgagagt
     9781 gagctatgca ttgaaacacc caaactcact acaagtgagg aaaagggcaa aaagtcgagc
     9841 aagagctgtg ggtcaagtag ccatgagaac cgtcccctgg acctgctaca caagatggag
     9901 tcaaagagct ccaaccagct ttcctggctc tcagtatcca tggatgcagc cctaggctgc
     9961 aggactaata tatttcagat ccagcgttca ggggggcgta aacataccga gaagcatgca
    10021 agcggtggct ccaccgtcca catccatccc caagctgctc ctgttgtctg cagacacgtt
    10081 ttggatacac tcattcaatt ggccaaggta tttcccagcc acttcacaca gcagcggacc
    10141 aaagaaacaa actgtgagag tgatcgggaa aggggcaata aggcctgtag cccatgctcc
    10201 tcacagtcct ccagcagtgg catttgcaca gacttctggg acttattggt aaaactggac
    10261 aacatgaatg tcagccggaa aggcaagaac tccgtgaagt cagtgccagt gagcgctggc
    10321 ggtgaggggg aaacctctcc atacagcctc gaggcctctc cactggggca gctcatgaac
    10381 atgttgtcac acccagtcat ccgccggagc tctctcttaa ctgagaaact cctcagactc
    10441 ctttctctca tctcaattgc tctcccagaa aacaaggtgt cagaagcaca ggctaattct
    10501 ggcagcggtg cttcctccac caccactgcc acctcaacca catctaccac caccaccact
    10561 gccgcctcca ccacgcccac accccctact gcacccaccc ctgtcacttc tgctccagcc
    10621 ctggttgctg ccacggctat ttccaccatt gtcgtagctg cttcgaccac agtgactacc
    10681 cccacgactg ctaccactac tgtttcaatt tctcccacta ctaagggcag caaatctcca
    10741 gcgaaggtga gtgatggggg cagcagcagt acagacttta agatggtgtc ctctggcctc
    10801 actgaaaacc agctacagct ctctgtagag gtgttgacat cccactcttg ttctgaggaa
    10861 ggcttagagg atgcagccaa cgtactactg cagctctccc ggggggactc tgggacccgg
    10921 gacactgttc tcaagctgct actgaatgga gcccgccatc tgggttatac cctttgtaaa
    10981 caaataggta ccctgctggc cgagctgcgg gaatacaacc tcgagcagca gcggcgagcc
    11041 caatgtgaaa ccctctctcc tgatggcctg cctgaggagc agccacagac caccaagctg
    11101 aagggcaaaa tgcagagcag gtttgacatg gctgagaatg tggtaattgt ggcatctcag
    11161 aagcgacctt tgggtggccg ggagctccag ctgccttcta tgtccatgtt gacatccaag
    11221 acatctaccc agaagttctt cttgagggta ctacaggtca tcatccagct ccgggacgac
    11281 acgcgccggg ctaacaagaa agccaagcag acaggcaggc taggttcctc cggtttaggc
    11341 tcagctagca gcatccaggc agctgttcgg cagctggagg ctgaggctga tgccattata
    11401 caaatggtac gtgagggtca aagggcgcgg agacagcaac aagcagcaac gtcggagtct
    11461 agccagtcag aggcgtctgt ccggagggag gaatcaccca tggatgtgga ccagccatct
    11521 cccagtgctc aagatactca atccattgcc tccgatggaa ccccacaggg ggagaaggaa
    11581 aaggaagaaa gaccacctga gttacccctg ctcagcgagc agctgagttt ggacgagctg
    11641 tgggacatgc ttggggagtg tctaaaggaa ctagaggaat cccatgacca gcatgcggtg
    11701 ctagtgctac agcctgctgt cgaggccttc tttctggtcc atgccacaga gcgggagagc
    11761 aagcctcctg tccgagacac ccgtgagagc cagctggcac acatcaagga cgagcctcct
    11821 ccactctccc ctgccccctt aaccccagcc acgccttcct cccttgaccc attcttctcc
    11881 cgggagccct catctatgca catctcctca agcctgcccc ctgacacaca gaagttcctt
    11941 cgctttgcag agactcaccg cactgtgtta aaccagatcc tacggcagtc cacgacccac
    12001 cttgctgatg ggccttttgc tgtcctggta gactacattc gtgtcctcga ctttgatgtc
    12061 aagcgcaaat atttccgcca agagctggag cgtttagatg aggggctccg gaaagaagac
    12121 atggctgtgc atgtccgtcg tgaccatgtg tttgaagact cctatcgtga gctgcatcgc
    12181 aaatcccccg aagaaatgaa gaatcgattg tatatagtat ttgaaggaga agaagggcag
    12241 gatgctggcg ggctcctgcg ggagtggtat atgatcatct ctcgagagat gtttaaccct
    12301 atgtatgcct tgttccgtac ctcacctggt gatcgagtca cctacaccat caatccatct
    12361 tcccactgca accccaacca cctcagctac ttcaagtttg tcggacgcat tgtggccaaa
    12421 gctgtatatg acaaccgtct tctggagtgc tactttactc gatcctttta caaacacatc
    12481 ttgggcaagt cagtcagata tacagatatg gagagtgaag attaccactt ctaccaaggt
    12541 ctggtttatc tgctggaaaa tgatgtctcc acactaggct atgacctcac cttcagcact
    12601 gaggtccaag agtttggagt ttgtgaagtt cgtgacctca aacccaatgg ggccaacatc
    12661 ttggtaacag aggagaataa gaaggagtat gtacacctgg tatgccagat gagaatgaca
    12721 ggagccatcc gcaagcagtt ggcggctttc ttagaaggct tctatgagat cattccaaag
    12781 cgcctcattt ccatcttcac tgagcaggag ttagagctgc ttatatcagg actgcccacc
    12841 attgacatcg atgatctgaa atccaacact gaataccaca agtaccagtc caactctatt
    12901 cagatccagt ggttctggag agcattgcgt tctttcgatc aagctgaccg tgccaagttc
    12961 ctccagtttg tcacgggtac ttccaaggta cccctgcaag gctttgctgc cctcgaaggc
    13021 atgaatggca ttcagaagtt tcagatccat cgagatgaca ggtccacaga tcgcctgcct
    13081 tcagctcaca catgttttaa tcagctggat ctgcctgcct atgagagctt tgagaagctc
    13141 cgccacatgc tactgttggc tatccaggag tgctctgaag gctttgggct ggcctaataa
    13201 ggccctgccc aactccgtgg ggtttttttt accattgttg gacctgggga ggggggagtt
    13261 aaaaaaagaa ccagaaagaa attgtcaaaa accaataaat gaaatccacc aactcaccgt
    13321 gtgtgtccca gctgccccat cttccccagc gcatacctgt tcctcttctc attctctccc
    13381 cgccgcctgt ttcctcacct tctctcccct ttccatgccg tccatgatcc ccaccccatg
    13441 tgttttaaaa aggcagtagc ctttgcaggg acctgtctgt cccaactgtt tgaacagtgt
    13501 gctcctcaga ttctgtgttc agaaggattt gctgcattga gacttgaaac ctttggatag
    13561 gggaaaaaat tatatatata tatatttttt tgttctgttt gcatttctta atttgtgctt
    13621 ggaatgtgtt gatgtgcaca gctaatgatt caatgcgaga caagattggc gtctgtgttg
    13681 tggaggtttc aaataaagag cactcttcat aactcacttt tcacaatgga gtttttttca
    13741 aacttaaaaa aaaaaacaaa aaactcttaa gcacatgcta ggcatctgga gaaaagatgg
    13801 attctccagg aaaccatccc tgccctaccc gcttgcccac ctgcctccac cactggtgca
    13861 acttcctcct tcagagtcag tttctgcagt caggagagat gagcagttca ccaggaattg
    13921 ggggtggggg catctgtttt ttttttttag aatgggagct gcacttgggg atggtgatgg
    13981 tgttagagat tgcagcccag gacaaagcat caccttgctc aaggggaaca aactgcagca
    14041 ggttgtacaa aacattcaga gagctcactt gacccaaccg agggttttac ttggtgagcc
    14101 tttctagtag tcttgagtct ggggctcagt tttagatttt tatttcatta atgtttgttt
    14161 atttctaata aattttaata agcgagtaat ttaactaggt cgccagaaga ccattatttt
    14221 tgtttgtcta tttttgttgt cgtcactccc tctctcgtca tctgtctgcc agtctgttca
    14281 cagatcacct tgtagtccct tgtggttttt actccatcca gtgcttaaag ctgatctaag
    14341 gacttcgatg gttccagcct tcaaagaaaa aataatttaa taaaaattta gttagaaaaa
    14401 ataaccccaa aaaaaaaaaa aaaaaaaaaa aaaaagggcg gccgcactcg agatatctag
    14461 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
    14521 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg
    14581 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
    14641 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
    14701 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
    14761 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
    14821 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
    14881 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
    14941 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
    15001 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgtccctg
    15061 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
    15121 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
    15181 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
    15241 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
    15301 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
    15361 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
    15421 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
    15481 gagtttttct aatcagaatt ggttaattgg ttgtaacatt attcagattg ggccccgttc
    15541 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    15601 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    15661 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    15721 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    15781 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    15841 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    15901 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    15961 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    16021 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    16081 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    16141 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    16201 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    16261 gataaccgta ttaccgctag catggatctc ggggacgtct aactactaag cgagagtagg
    16321 gaactgccag gcatcaaata aaacgaaagg ctcagtcgga agactgggcc tttcgtttta
    16381 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
    16441 acgttgtgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
    16501 atcaaactaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttc
    16561 ctgttagtta gttacttaag ctcgggccc
//
