LOCUS       sequence-182553-        4887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Epifano O, Liang LF, Familari M, Moos MC, Dean J
  TITLE     Coordinate expression of the three zona pellucida genes during mouse
            oogenesis.
  JOURNAL   Development. 1995 Jul . 121(7):1947-56.
  PUBMED    7635043
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 1995 Jul . 121(7):1947-56."
COMMENT     Sequence Label: Mouse ZP1 (JD#264)
FEATURES             Location/Qualifiers
     source          1..4887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1954..1973)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1955..1973)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1980..1997)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1980..1996)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1989..2011)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(2138..2593)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2275..2296)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2487..2506
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2620..2724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2725..2793,2794..3585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2943..2962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3756..4344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4245..4264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4498..4515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4632..4653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4668..4673,4674..4691,4692..4698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4706..4722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4711..4733
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4730..4746
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4730..4746
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4767..4785
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     4822..4838
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4822..4838
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atggcctggg gttgttttgt ggtcctgctt ctgctggcgg cagctcccct gagattgggt
       61 cagcgtctgc atctcgagcc tggctttgaa tacagctatg actgtggggt acggggcatg
      121 cagctgctgg tgttccctag gccaaaccag actgtccaat tcaaggtgct ggatgaattt
      181 gggaaccggt ttgaggtgaa taactgctct atctgctacc actgggtgac cagtgaggcc
      241 caggagcaca cagtattctc agctgattac aaaggctgcc acgttctaga gaaggatggg
      301 cggttccacc tgagggtgtt catacaagcc gtcctaccca atggccgtgt ggatatagca
      361 caagatgtca cactgatctg tcccaaacca gaccacaccg tgactccgga cccctacctg
      421 gctccaccca ccacacctga gccttttaca cctcatgctt ttgcccttca ccccatccct
      481 gaccacacct tggctgggtc tggccacact ggtctcacta cgttgtaccc agaacagagc
      541 ttcatccatc caactcctgc tccgccatcc ctaggacctg gacctgctgg atccactgta
      601 cctcactccc aatggggcac gctggagccc tgggaattga ctgagctgga ttctgtaggt
      661 acccatctgc cccaggagcg gtgccaggta gcctccgggc acatcccatg catggtaaat
      721 ggaagttcca aggaagcctg tcagcaggct ggctgctgct atgacagtac caaagaagag
      781 ccctgttact atggcaacac agtcactctc cagtgtttca aaagtggcta ctttaccttg
      841 gtcatgtcac aagaaacagc cttgacacat ggagtcctgc tagacaatgt ccacctggcc
      901 tatgccccca acggatgccc ccctacccag aagacaagtg cttttgtggt cttccatgtc
      961 cctctcaccc tctgtggaac agcaatccag gtggtcggcg aacaactcat ctatgagaac
     1021 cagctggtgt ctgacattga tgtccaaaag gggccacagg gttccatcac tcgggacagt
     1081 gccttccggc ttcatgttcg ctgcatcttc aatgctagcg atttcctgcc catccaggca
     1141 tctatcttct caccccaacc acctgcccct gtgactcaat ctggacccct gaggctggag
     1201 ctgaggattg ccacggataa gactttcagc tcctactatc aggggagtga ctatcccctt
     1261 gtgagactgc tccgggaacc agtctatgta gaggtccgac tcctgcagag aactgatccc
     1321 agtctggttc tgttgctaca ccagtgctgg gccacgccca ccaccagccc ctttgagcag
     1381 ccccaatggc ctattctgtc agatgggtgt cctttcaagg gtgacaacta cagaacacaa
     1441 gtagtggccg cagacaagga ggcgcttccc ttctggtccc actatcagcg cttcaccatc
     1501 accaccttca tgctccttga ctccagctcc cagaatgccc ttaggggaca ggtctatttc
     1561 ttctgtagtg cctctgcctg ccaccctctg gggtctgata catgctctac tacgtgtgac
     1621 tctgggatag caaggcgtcg acgatcctct ggtcaccaca acatcactct ccgggccctg
     1681 gacattgtaa gctctccagg ggcagtgggc tttgaggatg ctgctaaact tgagccctca
     1741 ggttccagca ggaactccag ctcaagaatg ctgctcctgc tgctggccat caccctagcc
     1801 ctggcggccg ggatctttgt gggtctgatc tgggcctggg cccagaagct ctgggaaggc
     1861 atcagatatt aaatggactc aataaaccat tgtttgaaaa ctcctgaaaa aaaaaaaaaa
     1921 aaaaactcga gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacaattca
     1981 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2041 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     2101 ccttcccaac agttgcgcag cctgaatggc gaatggcaaa ttgtaagcgt taatattttg
     2161 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     2221 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     2281 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     2341 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     2401 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     2461 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     2521 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     2581 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2641 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2701 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     2761 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2821 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2881 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2941 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3001 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3061 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3121 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3181 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3241 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3301 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3361 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3421 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3481 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3541 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     3601 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     3661 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3721 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3781 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3841 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3901 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3961 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4021 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4081 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4141 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4201 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4261 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4321 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4381 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4441 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4501 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4561 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4621 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     4681 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     4741 tatgaccatg attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggag
     4801 ctccaccgcg gtggcggccg ctctagaact agtggatccc ccgggctgca ggaattcggc
     4861 acgagctgac agacagggac ctccatc
//
