LOCUS       sequence-182562-        3877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3877)
  TITLE     Additional Tabin lab plasmids
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBS cHoxD11 (CT#341)
FEATURES             Location/Qualifiers
     source          1..3877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1067..1083
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1067..1083
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2035..2051)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2036..2052)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2076..2095)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2077..2095)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2102..2119)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2102..2118)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2111..2133)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(2260..2715)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2397..2418)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2609..2628
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2742..2846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2847..2915,2916..3707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3065..3084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg
     1081 atcccccggg ctgcaggaat tcgcggccgc attgaagcct cccggtcgct cgcaatcgcg
     1141 aaaaggaaga catgaccgag tttgacgatt gcagtcacgg cgcatccaat atgtatctgc
     1201 cagggtgcgc ttattacgtc tcgccctcag acttctcgac gaaaccctct tttttgtcgc
     1261 aatcgtcttc ctgtcaaatg acttttcctt attcttctaa tttacctcat gtccaacctg
     1321 tgcgcgaagt ggcattcagg gaatacggat tagagcgcgg caaatggcag tacaggggca
     1381 gttacgctcc ctattactca gctgaagagg tgatgcacag agactttctg cagcccgcga
     1441 acaggaggac ggaaatgcta ttcaaaacgg accccgtgtg cggccaccac ggagcgtccc
     1501 ccgccgcctc caacttctac ggctcggtgg ggaggaacgg catcctgccg cagggcttcg
     1561 accagttcta cgagccggcc cccgcgcccc cgtaccagca gcactccgag ggcgaggggg
     1621 acgcggacaa gggcgagctg aagccccagc acagcgcctg cagcaaggca ccttccggcc
     1681 aggagaagaa agtgacaacg gcgagcggcg ccgcttcccc tcccggtgag gctgttgcag
     1741 agaagagcaa cagttcagca gttcctcagc gatcgaggaa aaagaggtgt ccctatacca
     1801 aatatcagat aagagaactg gaacgtgagt ttttcttcaa cgtgtacata aacaaagaga
     1861 aaagactgca gttgtccaga atgctcaacc tcactgaccg gcaagttaaa atctggttcc
     1921 agaatcgaag gatgaaagaa aagaaactga acagagatcg actccaatac ttcactggaa
     1981 atcccttgtt ttgaggagga aaaaaagcgg ccgcgaattc gatatcaagc ttatcgatac
     2041 cgtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacaatt
     2101 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     2161 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     2221 gcccttccca acagttgcgc agcctgaatg gcgaatggca aattgtaagc gttaatattt
     2281 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     2341 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     2401 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     2461 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     2521 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     2581 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     2641 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     2701 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2761 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2821 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2881 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2941 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     3001 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     3061 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3121 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     3181 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3241 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3301 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3361 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3421 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3481 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3541 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3601 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3661 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3781 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3841 gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
