LOCUS       sequence-182575-        4564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E.coli UTI89 FimH signal peptide and lectin domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Svenningsen
  TITLE     Svenningsen-FimH plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD-FimH-9xHis
FEATURES             Location/Qualifiers
     source          1..4564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             312..334
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             883..909
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(965..982)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1135..1221
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1313..1340
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1359..1450
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1451..1519,1520..2311)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1669..1688)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2482..3070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2971..2990
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3224..3241
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3256..3396
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3322..3341)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3482..3504
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3660..4538)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagaaataa ttttgtttaa ctttaagaag gagatataca tacccatgaa acgtgtgatt
      361 accctgtttg cagttctgct gatgggttgg agcgttaatg catggtcatt tgcatgtaaa
      421 accgcaaatg gcaccgcaat tccgattggt ggtggtagcg caaatgttta tgttaatctg
      481 gcaccggcag ttaatgttgg tcagaatctg gttgttgatc tgagcaccca gattttttgc
      541 cataatgatt atccggaaac catcaccgat tatgttaccc tgcagcgtgg tgcagcatat
      601 ggtggtgttc tgagcagctt tagcggcacc gtgaaatata acggtagcag ctatccgttt
      661 ccgaccacca gtgaaacacc gcgtgttgtg tataatagcc gtaccgataa accgtggcct
      721 gttgcactgt atctgacacc ggttagcagt gccggtggtg ttgcaattaa agcaggtagc
      781 ctgattgccg ttctgattct gcgtcagacc aataactata actccgatga ttttcagttt
      841 gtgtggaaca tctatgccaa taatgatgtt gttgttccga cgcatcatca ccaccatcac
      901 catcatcatt gagtttaaac ggtctccagc ttggctgttt tggcggatga gagaagattt
      961 tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg
     1021 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
     1081 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata
     1141 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1201 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     1261 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc
     1321 atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca
     1381 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1441 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1501 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1561 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1621 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1681 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1741 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1801 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1861 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1921 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1981 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2041 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2101 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2161 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2221 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2281 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2341 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2401 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2461 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2521 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2581 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2641 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2701 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2761 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2821 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2881 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2941 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3001 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3061 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3121 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3181 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3241 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3301 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     3361 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     3421 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3481 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag
     3541 atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat ggacgaagca
     3601 gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat
     3661 tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc
     3721 gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca
     3781 acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg
     3841 ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga
     3901 tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg
     3961 ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt
     4021 ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt
     4081 atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac
     4141 aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat
     4201 attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg
     4261 tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac
     4321 agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga
     4381 ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa
     4441 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag
     4501 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
     4561 agag
//
