LOCUS       sequence-182584-        4140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry TOPO TA cloning vector for ETS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label: pCR8/GW/TOPO-ETS1
FEATURES             Location/Qualifiers
     source          1..4140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1483..1582)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1599..1618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1600..1618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1623..1639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1623..1639)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2070..2861
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2954..3542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3443..3462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3696..3713
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3872..3899)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3991..4077)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4126..4140,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4140,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccgaattc gcccttacca tgaaggcggc cgtcgatctc aagccgactc
      181 tcaccatcat caagacagaa aaagtggatc tcgagctttt cccttccccg gacatggaat
      241 gtgcagatgt cccgctgtta actccgagca gcaaagaaat gatgtcccaa gccttgaaag
      301 ctactttcag tggtttcaca aaagaacagc agcggctggg aatccccaaa gacccccggc
      361 agtggacaga aacccacgtc cgggactggg tgatgtgggc tgtgaatgag ttcagcctga
      421 aaggtgtgga cttccagaag ttctgtatga gtggagcagc actgtgtgcc ctgggtaaag
      481 aatgcttcct cgagctggct ccagactttg ttggggatat cctgtgggag catctagaga
      541 tcctgcagaa agaggatgtg aaaccatatc aggttaatgg agccaaccct acctacccag
      601 aatcctgtta cacctcggat tacttcatca gctacggtat cgagcatgct cagtgtgttc
      661 ctccctcaga gttctcagag cccagcttca tcacagagtc ctatcagacg ctgcatccta
      721 tcagctcaga agaactcctg tccctcaagt atgagaacga ctacccttct gtcattctcc
      781 aggaccctct ccagacagac accttgcaga cagactactt tgccatcaag caagaggtgt
      841 taactccaga caacatgtgc ctggggagag ccagtcgtgg taaactcggg ggccaggact
      901 cttttgagag cgtagagagc tacgatagtt gtgaccgcct cacccagtcc tggagcagcc
      961 agtcatcctt caacagcctg cagcgggtcc cctcctatga cagcttcgac tacgaggatt
     1021 atcccgctgc cctgcccaac cacaagccca agggcacctt caaggactat gtgcgtgacc
     1081 gtgctgacct caacaaggac aagcctgtca ttcctgctgc tgccctggct ggctacacag
     1141 gaagtgggcc gatccagctg tggcagtttc ttctggaatt actcactgat aagtcttgtc
     1201 agtcctttat cagctggaca ggagatggct gggaattcaa gctttctgac ccagatgagg
     1261 tggccaggag atggggaaag aggaaaaaca aacctaagat gaattatgag aaactgagcc
     1321 gtggccttcg ctactattat gacaaaaata tcatccacaa gacggcgggc aagcgctacg
     1381 tataccgctt tgtgtgcgac ctgcagagcc tgctgggata cacccctgag gagctgcacg
     1441 ccatgctgga cgtaaagccg gatgctgaca agggcgaatt cgacccagct ttcttgtaca
     1501 aagttggcat tataaaaaat aattgctcat caatttgttg caacgaacag gtcactatca
     1561 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     1621 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     1681 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga
     1741 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     1801 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     1861 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     1921 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     1981 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2041 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     2101 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2161 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     2221 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2281 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2341 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2401 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2461 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2521 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     2581 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     2641 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     2701 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     2761 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     2821 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     2881 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     2941 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3001 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3061 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3121 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3181 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3241 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3301 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3361 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3421 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3481 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3541 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3601 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3661 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3721 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3781 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     3841 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     3901 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     3961 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     4021 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     4081 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
//
