LOCUS       sequence-182590-        8435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Gao L, Cox DBT, Yan WX, Manteiga JC, Schneider MW, Yamano T, 
            Nishimasu H, Nureki O, Crosetto N, Zhang F
  TITLE     Engineered Cpf1 variants with altered PAM specificities.
  JOURNAL   Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900.
  PUBMED    28581492
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900."
COMMENT     Sequence Label: pAsCpf1(TYCV)(BB) (pY211)
FEATURES             Location/Qualifiers
     source          1..8435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        118..403
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        complement(527..775)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(585..604)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(755..775)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     repeat_region   788..917
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(979..1567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1059..1078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1738..2529,2530..2598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2361..2380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2599..2703)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2771..2789
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2827..2849)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2949..2968
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2985..3440)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3122..3143)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3334..3353
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3515..3655
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3526..3655
                     /label=AAV2 ITR
     polyA_signal    3664..3871
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3860..3877
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3905..3985)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(3992..4039)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(4040..7957)
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPRCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4311..4320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7982..8002)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8005..8014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          8046..8273
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     promoter        complement(join(8274..8435,1..116))
                     /label=chicken beta-actin promoter
ORIGIN
        1 cccccccccc gcccccatcg ctgcacaaaa taattaaaaa ataaataaat acaaaattgg
       61 gggtggggag gggggggaga tggggagagt gaagcagaac gtggggctca cctcgaccat
      121 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
      181 gtactgggca caatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
      241 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
      301 ttgacgtcaa tggaaagtcc ctattggcgt tactattgac gtcaatgggc gggggtcgtt
      361 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgggtaccc gcgtatatct
      421 ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaata
      481 gaaaaaaagg tcttctcgaa gacccatcta caagagtaga aattacggtg tttcgtcctt
      541 tccacaagat atataaagcc aagaaatcga aatactttca agttacggta agcatatgat
      601 agtccatttt aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac
      661 gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa attaattcca attatctctc
      721 taacagcctt gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctctctag
      781 aggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
      841 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggcg gcctcagtga gcgagcgagc
      901 gcgcagctgc ctgcaggaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1021 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1081 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1141 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1201 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1261 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1321 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1381 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1441 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1501 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1861 tgctgcaatg ataccgcggc ttccacgctc accggctcca gatttatcag caataaacca
     1921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2221 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2281 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2341 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2401 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2461 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     2521 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     2581 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     2641 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     2701 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     2761 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     2821 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     2881 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     2941 taactatgcg gcatcagagc agattgtact gagagtgcac cataaaattg taaacgttaa
     3001 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaatagac
     3061 cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatagagt tgagtgttgt
     3121 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3181 aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg
     3241 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3301 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     3361 taaggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     3421 tgcgccgcta cagggcgcgt actatggttg ctttgacgta tgcggtgtga aataccgcac
     3481 agatgcgtaa ggagaaaata ccgcatcagg cgcccctgca ggcagctgcg cgctcgctcg
     3541 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
     3601 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc
     3661 cgctccccag catgcctgct attctcttcc caatcctccc ccttgctgtc ctgccccacc
     3721 ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat
     3781 taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa
     3841 acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgagc tctaggaatt
     3901 cttaggcata gtcggggaca tcatatgggt atgcataatc aggcacgtcg tagggataag
     3961 cgtaatctgg aacatcgtat gggtaggatc cctttttctt ttttgcctgg ccggcctttt
     4021 tcgtggccgc cggccttttg ttgcgcagct cctggatgta ggccagccag tcctgattgg
     4081 agatgccgtt ctgcagcttc agatccttgc tctccttcag gtgattcagc agcagctggc
     4141 ccttcagggc gatgtggtag gcgccattgg catcggcgtc catgggccac tctgggttct
     4201 gaaaccggga gtcgaagcac acgccattca gatcgcgcac ggggctgttg atatagtcct
     4261 cgcctgtggc ggcattggag ttccgcatct gcagcacgct gcggatcagg gccaccatgg
     4321 tgtcgatggc gtgagaatcg tcattctcca gcagctttgg caggatgttg gagccatccc
     4381 tgaacacgat gcccttctcc tccagcaggg cgatcagctc gttggcagga tacaggtccc
     4441 ggtatctgcc ggtgaatctg tgattctcga tcactggcac gattctcttg ccggcgatga
     4501 aaggggtgcc cttggcgtca aactgtgtct cgttcttctc gaacacgata tcccatgcag
     4561 gcataaagcc gggcaggccc ctctggaagg acagatttct gttcatctta aagtgcagga
     4621 tgaagtcgcc ggttttcacg tcgtagtgca gaaagtcgaa gccctccagg aagtgcttgc
     4681 ggctctcgtg attcttgatg gttttccaca cgaaggggtc cacgaagccg gtcaggggat
     4741 cgatcttaga tgtatatggg gcaggcacgt aaaacaggaa gccagactgg gtgcccatct
     4801 tggcaaagga ggtgaactgg tctgtcagct ggtatgggtt cagcacgcct cccactttct
     4861 ctgctggata gtccttcagc accaggcaat tcagcttatc gatcagcatc ttctcgaact
     4921 gctggtacac ggccttctcg gcgatgccgg tcctcttgct cttaaagccg aaattcaggt
     4981 tctccagcac caccacggcc tggtagtgga tcatcaggtc cacgatctcg tggatgacct
     5041 ggctcagata gccctgcttc agatccttga ttgtgcccac cacagaccag gcctgccttg
     5101 ctgccaccct ctccttctcc ctgttgtcca gcttcttctg gtaatcaaac tgctggatgg
     5161 tgttcaggct ccgctgctcc aggatcttgc cggtggagtc gatcactgtg atatagatca
     5221 ggtttctctc gccccgatcg atgccgatga taggtgtctc ggggtgctcc ttcaggtagg
     5281 cattcaccct ctggttgaac ttagatgggg aattggcggc ctgatagttc agtgtgatag
     5341 gcacgtggaa aaagaacttg tcgctggtaa agcgcctatc cttgatgatc tcgtgagaca
     5401 cctccttggt gatcacgttg ggcagcaggg ccctggcctc atcagacagg tcgtgggaca
     5461 gtctgtgatt cacatagtcg tacagctcct ggtacagggt gtcggggatt ggggttttct
     5521 gatccttcag cttcttgttc agcatcttct ctcccagccg gtgtgccatc ctcttcatcc
     5581 tggacttagg gcggtagaac agctcggcct ggccattcag cttgatgctt gtcttggcca
     5641 ggttctctgg agaaaacaga ccggtccaat acagtgtgtg cagattaggc ttgccgtggt
     5701 ggcccttggc aaagtccttg ttatagatct ggaacaggta cagcttgcct gtctccacgg
     5761 catccatgat ctccttctcg gcgattctct ggaagctgat gtggtacagc aggggattca
     5821 gctcggcata gtactcgccc aggtccttat actgagagga tggccgcagg ctagacagat
     5881 cgatagaggt tgtcttggta tacttggaca gaaaatccct tgtgaagtcg atccacttgc
     5941 acagggcctc tctgtagccc ttctggtcgc cggttttctt ggcgtacgct gtctgaaact
     6001 tctttggctc cttctcagga ttgttcaggt cgtagatctc ctttgtgatc tccagaggct
     6061 cgatgaaatt gttggacagc aggatggggg ttgtgtgggt ctgaaagtgg gcggtcaccg
     6121 ccttcagctg ggtgctgcac cgtgggatca tcttggcggc atccggaaag tagtcatagt
     6181 acatcttatc aaagccctcg ctggttttct ctgtgggctc gaagctgagg gccttatacc
     6241 tgcccttctg ctttggcatg atgcccagat agtacaggcc gttcttcaca aacaggatgg
     6301 cgccattgtt cttctcctta ttcacgtccc agccccgggc cagtgtaggc atctgaaagt
     6361 tcagcttgaa cttctccacg gagtagggct tcttggtggc ataatttctg gccttgttgt
     6421 agaagctcag agaaggctcc atctccagct tgatgccggt cagccgggca gagaactcgg
     6481 ggtccacctc gttggactca tccacggcaa accagtccag caggtggtac aggcccagca
     6541 ggctgtccag ctgagacttc aggatctcct tctcctcctg cttcttcagg gttgtaggca
     6601 gtggctgatc cagggcggcg tgtgcgtggg acaggatctc gctggttttc tgcttgaagg
     6661 cctcgctcag ctccttgcct gcggcagaga tgatctcctg caggttgata tcctcgtgct
     6721 tcaggctgcg ctgcaccttc tccttggcag acttggtgat cttgcctgtc agctcggaga
     6781 ttctccgctc atacagggca ttcctcagtg tatcccagtg gtcgcacagg gcgctgctga
     6841 ttgtctccag cttcttgtgg ctgatgaaga tgtgtgtcag gtcgatgctg ttcagctcgt
     6901 taaacagggc ctcggctgtc tccagcacgt tctcgtttct cagcagtgtc ttgtacttgc
     6961 agaaggactg gatcacttcc tcgtcgctct taaactcctc caggatgaaa gacagggtgt
     7021 tcctatcgga caggatctgc ttaaacaggg ggatgaatct gtgtggcagg gaggcgatga
     7081 tgtgggctgt ctcatcattc ttctggatgg ccagattcag cacctcgttc aggcccttga
     7141 tcttctcggt gcctgcctcc cgagagattc ctcccagcag ctggttatac aggtcgatct
     7201 gggtctgtgt cagcagctgg ttataaaaag ggaaggaaaa cacctcctcg atggaggtgc
     7261 tcacgaagat gccgatggcc ttcttcacgt tctcaaagtg ctcccgcagg ctgggcacgg
     7321 cggtgatcag gcgtgtgaag atgtgacaat tctccttaaa cttggggaag ttgtcctgca
     7381 cgatgcggtg tgggatggct gtgctgatat cctcggcgct gaacacgttc ttcctgttct
     7441 cataaaagcc ggagaagtag gttgtaaact tgtcgaagct ccgcagcagg gcgttctcgt
     7501 gctcggttgt ggtcacggtg cccagctgct tcagcacctt gccattaaac agctcggcct
     7561 tgaacaggcc cttgtagatc tcggcgtgtc tcttattgat ggcatcggtc aggttgtctg
     7621 tccggccgat gaagtagtcg tggatggcat tgcgatatgt ggcctgctcc tcgatcaggg
     7681 cgttccttgt ctcctcggtt ttctcctttc tataggagtc gatggcggcg ctcaggttct
     7741 cccaatccag ctgcaccagc tgcaggcact ggtcggcata ggtcttgtag atccgatcga
     7801 tgatgggctt cagctccttg tagtgatcat tgcgggcctt gtcctcctcg atgaagccct
     7861 gctcctggat gtgcttcagg gtcttgccct gtgggatcag ctcaaaccgc agtgtcttgc
     7921 tcacctgata caggttggta aagccctcga actgtgtggc tgctgggact ccgtggatac
     7981 cgaccttccg cttcttcttt ggggccatgg tggcaagctt aagtttaaac gctagccggt
     8041 ccaacctgaa aaaaagtgat ttcaggcagg tgctccaggt aattaaacat taatacccca
     8101 ccaaccaacc atcccttaaa cccttacctc ttgctcagct aattacagcc cggaggagaa
     8161 gggccgtccc gcccgctcac ctgtgggagt aacgcggtca gtcagagccg gggcgggcgg
     8221 cgcgaggcgg cggcggagcg gggcacgggg cgaaggcagc gcgcagcgac tcccgcccgc
     8281 cgcgcgcttc gctttttata gggccgccgc cgccgccgcc tcgccataaa aggaaacttt
     8341 cggagcgcgc cgctctgatt ggctgccgcc gcacctctcc gcctcgcccc gccccgcccc
     8401 tcgccccgcc ccgccccgcc tggcgcgcgc ccccc
//
