LOCUS       sequence-182599-        8827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of copGFP-T2A-FLuc under control of a 
            COL2A1-based, chondrogenesis-responsive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   De La Vega RE, Scheu M, Brown LA, Evans C, Ferreira E, Porter RM
  TITLE     Specific, sensitive and stable reporting of human mesenchymal 
            stromal cell chondrogenesis.
  JOURNAL   Tissue Eng Part C Methods. 2019 Feb 6. doi: 
            10.1089/ten.TEC.2018.0295.
  PUBMED    30727864
REFERENCE   2  (bases 1 to 8827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Tissue Eng 
            Part C Methods. 2019 Feb 6. doi: 10.1089/ten.TEC.2018.0295."
COMMENT     Sequence Label: pGF1-4eCOL2A1
FEATURES             Location/Qualifiers
     source          1..8827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             275..940
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             275..940
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             1082..1135
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1142..2794
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1208..1226)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    2805..3393
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2858..2878)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3276..3287)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3484..3717
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3789..3910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3826..3845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3880..3899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3950..4085
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4118..4134)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4118..4134)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4131..4153)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4142..4158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4166..4172,4173..4190,4191..4196))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4211..4232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4349..4366)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4520..5108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4600..4619)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5279..6070,6071..6139))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5902..5921
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6140..6244)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6312..6330
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     6490..6509
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6703..6725
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6717..6734
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6718..6734
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6749..6975
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6976..7156
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7203..7328
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7821..8054
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8239..8283
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8550..8666
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 catgagagct gtgaatcggg ctctgtgtgc gctcgagaaa agccccattc atgagagcta
       61 gccggcttgg gcaggtgcgg gcactggcag ggcccaggcg ggctccgggg gcgggcggtt
      121 caggttacag cccagctggg ggcagggggc ggcccgcggt ttgggcgagt tcgccagcct
      181 cgaaaggggc cgggcgcata taacgggcgc cgcggcgggg agaagacgca gagcgctgct
      241 gggctgccgg gtctcccgct tcggatccgc caccatgccc gccatgaaga tcgagtgccg
      301 catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc
      361 cgagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc
      421 ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg
      481 ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga
      541 gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg
      601 cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac
      661 cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt
      721 gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt
      781 ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg
      841 ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt
      901 ggagtaccag cacgccttca agacccccat cgccttcgcc agatctcgag atatcagcca
      961 tggcttcccg ccggcggtgg cggcgcagga tgatggcacg ctgcccatgt cttgtgccca
     1021 ggagagcggg atggaccgtc accctgcagc ctgtgcttct gctaggatca atgtgaccgg
     1081 tgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gcccttccgg
     1141 tatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccac tagaggatgg
     1201 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
     1261 tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc
     1321 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
     1381 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
     1441 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat
     1501 ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca
     1561 aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg
     1621 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
     1681 ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga actcctctgg
     1741 atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc
     1801 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
     1861 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
     1921 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta
     1981 caaaattcaa agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct
     2041 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc
     2101 gaaagaagtc ggggaggcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata
     2161 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     2221 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     2281 aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg
     2341 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     2401 tggagacata gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc
     2461 tttaattaaa tacaaaggat accaggtggc ccccgctgaa ttggagtcga tattgttaca
     2521 acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact
     2581 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     2641 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     2701 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     2761 aaaggccaag aagggcggaa agtccaaatt gtaatcgact cgacaatcaa cctctggatt
     2821 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2881 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2941 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3001 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3061 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3121 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3181 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     3241 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3301 cttcccgcgg cctgctgccg gctctgcggc ctcttccgca tcttcgcctt cgccctcaga
     3361 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattcgagc tcggtacctt
     3421 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     3481 gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt
     3541 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3601 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3661 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     3721 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3781 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3841 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3901 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     3961 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4021 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4081 aggcctagac ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa
     4141 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4201 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4261 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4321 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4381 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4441 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4501 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4561 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4621 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4681 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4741 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4801 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4861 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4921 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4981 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5041 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5101 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5161 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5221 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5281 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5341 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5401 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5461 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5521 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5581 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5641 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5701 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5761 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5821 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5881 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5941 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6001 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6061 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6121 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6181 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6241 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     6301 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     6361 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6421 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     6481 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     6541 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     6601 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     6661 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     6721 aaacgacggc cagtgccaag ctgacgcgtg tagtcttatg caatactctt gtagtcttgc
     6781 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     6841 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     6901 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     6961 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7021 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7081 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7141 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     7201 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     7261 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     7321 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     7381 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     7441 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     7501 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     7561 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     7621 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     7681 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     7741 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     7801 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     7861 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     7921 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     7981 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     8041 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     8101 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     8161 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     8221 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     8281 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     8341 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     8401 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     8461 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     8521 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
     8581 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     8641 aaacaaatta caaaattcaa aattttatcg atgaattcta gaactagtct gtgaatcggg
     8701 ctctgtgtgc gctcgagaaa agccccattc atgagagctg tgaatcgggc tctgtgtgcg
     8761 ctcgagaaaa gccccattca tgagagctgt gaatcgggct ctgtgtgcgc tcgagaaaag
     8821 ccccatt
//
