LOCUS       sequence-182610-        8891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant 
            Selection: nos:npt II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_38J
FEATURES             Location/Qualifiers
     source          1..8891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(100..915)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(100..915)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     823..842
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     907..926
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1206..1641
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    1788..1812
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(2089..2883)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2200..2219)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2810..2829
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2905..3088)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(2941..3120)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3699..4044
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3987..4007
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4166..4231
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4238..4258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4697..5002)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4799..4818)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6076..6132
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6136..6201
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6208..6228
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6556..6963)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     6813..6835
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6827..6844
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6828..6844
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6854..6873
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6854..6872
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6920..6940)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6920..6938)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6959..6975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6959..6975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6972..6994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6983..6999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7007..7013,7014..7031,7032..7037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7052..7073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             7259..7846
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      7860..8108
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    8115..8139
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8232..8820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8312..8331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
       61 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      121 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      181 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      241 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      301 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      361 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      421 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gccgaaatac
      481 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      541 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      601 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      661 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      721 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      781 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      841 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
      901 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      961 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1021 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1081 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1141 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1201 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1261 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     1321 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     1381 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     1441 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     1501 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     1561 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     1621 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     1681 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     1741 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcctgg caggatatat
     1801 tgtggtgtaa acgtgtacgc gttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
     1861 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     1921 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     1981 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     2041 acaggattca atcttaagaa actttattgc caaatgtttg aaaagctttc agaagaactc
     2101 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     2161 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     2221 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     2281 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     2341 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     2401 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     2461 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     2521 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     2581 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     2641 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     2701 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     2761 cgccgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     2821 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     2881 catggtcgac agatctagat ccggtgcaga ttatttggat tgagagtgaa tatgagactc
     2941 taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt
     3001 tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg
     3061 tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc
     3121 tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact
     3181 cccttaattc tccgctcatg atcggcgcgc cagatttgcc ttttcaattt cagaaagaat
     3241 gctaacccac agatggttag agaggcttac gcagcaggta tcatcaagac gatctacccg
     3301 agcaataatc tccaggaaat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
     3361 ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact
     3421 attccagtat ggacgattca aggcttgctt cacaaaccaa ggcaagtaat agagattgga
     3481 gtctctaaaa aggtagttcc cactgaatca aaggccatgg agtcaaagat tcaaatagag
     3541 gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc
     3601 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa
     3661 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta
     3721 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata
     3781 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt
     3841 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     3901 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
     3961 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     4021 agttcatttc atttggagag aacacggggg actcctgcag ggaattccaa gcaacgaact
     4081 gcgagtgatt caagaaaaaa gaaaacctga gctttcgatc tcttcggagt ggtttcttgt
     4141 tctttgaaaa agagggggaa gcatggatta taaggatcac gatggcgact acaaggacca
     4201 cgatattgac tacaaagacg atgacgataa aatggctcct aagaaaaagc gcaaagtcgg
     4261 tatccatggc gttccctcta gaatggtgga tctacgcacg ctcggctaca gtcagcagca
     4321 gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg aggcactggt
     4381 gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg cagcgttagg
     4441 gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga cacacgaaga
     4501 catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct tgctcacgga
     4561 tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg tgaagattgc
     4621 aaaacgtggc ggcgtgaccg caatggaggc agtgcatgcc agcaggaatg cgttaacagg
     4681 tgcccccctg gagacgttat attccccaga acatcaggtt aatggcgttt ttgatgtcat
     4741 tttcgcggtg gctgagatca gccacttctt ccccgataac ggaaaccggc acactggcca
     4801 tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac
     4861 gggagacttt atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg
     4921 gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat
     4981 aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcagcaaa agagccgttc
     5041 atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg
     5101 gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga gatattgaca
     5161 tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct
     5221 tcatagcata cctccttttg acatacttcg ggtatacata tcagtatata ttcttatacc
     5281 gcaaaaatca gcgcgcaaat acgcatactg ttatctggct tcgtctccaa cgaccacctc
     5341 gtcgccttgg cctgcctcgg cggacgtcct gccatggatg cagtgaaaaa gggattgccg
     5401 cacgcgccgg aattgatcag aagagtcaat cgccgtattg gcgaacgcac gtcccatcgc
     5461 gttgccggat cccagctggt gaagtccgag ctggaagaaa aaaagagcga gctgcgccac
     5521 aagctcaagt acgtgcccca cgagtacatc gagctgatcg agatcgcccg caacagcacc
     5581 caagaccgca tcctggagat gaaagtgatg gagttcttca tgaaggtgta cggctaccgc
     5641 ggcaagcacc tgggcggctc ccgcaagccc gatggcgcca tctacaccgt gggctccccc
     5701 atcgactatg gcgtcattgt cgacaccaag gcctactccg gcggctacaa cttacccatc
     5761 ggtcaggccg acgagatgca acgctacgtg aaggagaacc agacccgcaa taagcacatt
     5821 aatcccaacg agtggtggaa ggtgtacccc tcctccgtga ccgagttcaa attcctgttc
     5881 gtgtccggcc acttcaaggg caattataag gcccaactga cccgcctgaa ccgcaagacc
     5941 aactgcaacg gcgccgtgct gtccgtggag gaactgctga tcggcggcga gatgatcaag
     6001 gctggtaccc tgaccctgga agaggtgcgc cgcaagttca acaatggtga aatcaatttc
     6061 aggtccggaa gcggcgccac gaacttctct ctgttaaagc aagcaggtga cgtggaagaa
     6121 aacccagggc ccatggacta caaagaccat gacggtgatt ataaagatca tgacatcgat
     6181 tacaaggatg acgatgacaa gatggccccc aagaagaaga ggaaggtggg cattcacggg
     6241 gtgccggcta gcatggtgga tctacgcacg ctcggctaca gtcagcagca gcaagagaag
     6301 atcaaaccga aggtgcgttc gacagtggcg cagcaccacg aggcactggt gggccatggg
     6361 tttacacacg cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct
     6421 gtcacgtatc agcacataat cacggcgttg ccagaggcga cacacgaaga catcgttggc
     6481 gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct tgctcacgga tgcgggggag
     6541 ttgagaggtc cgccgttaca gttggacaca ggccaacttg tgaagattgc aaaacgtggc
     6601 ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg cactgacggg tgcccccctg
     6661 gagaccggcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     6721 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     6781 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     6841 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc cgccccttga
     6901 ggtcctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     6961 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7021 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7081 tgcgctcact gcccgctttc caccggtggt ctccaacgat catctggtgg cactcgcgtg
     7141 cctcggcgga cgtcctgcca tggatgcagt gaaaaaggga ttgccgcacg cgccggaatt
     7201 gatcagaaga gtcaatcgcc gtattggcga acgcacgtcc catcgcgttg ccagatctca
     7261 actagtcaaa agtgaactgg aggagaagaa atctgaactt cgtcataaat tgaaatatgt
     7321 gcctcatgaa tatattgaat taattgaaat tgccagaaat tccactcagg atagaattct
     7381 tgaaatgaag gtaatggaat tttttatgaa agtttatgga tatagaggta aacatttggg
     7441 tggatcaagg aaaccggacg gagcaattta tactgtcgga tctcctattg attacggtgt
     7501 gatcgtggat actaaagctt atagcggagg ttataatctg ccaattggcc aagcagatga
     7561 aatggagcga tatgtcgaag aaaatcaaac acgagacaaa catctcaacc ctaatgaatg
     7621 gtggaaagtc tatccatctt ctgtaacgga atttaagttt ttatttgtga gtggtcactt
     7681 taaaggaaac tacaaagctc agcttacacg attaaatcat atcactaatt gtaatggagc
     7741 tgttcttagt gtagaagagc ttttaattgg tggagaaatg attaaagccg gcacattaac
     7801 cttagaggaa gtgaggcgga aatttaataa cggcgagata aacttttaat agctcgagat
     7861 atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt
     7921 gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt
     7981 aagatcacat tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg
     8041 atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta
     8101 aagataagga gctctgacag gatatattgg cgggtaaact aagtcgctgt atgtgtttgt
     8161 ttgatttaaa tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8641 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8701 aagccagtta ccttcggaag aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8881 gggattttgg t
//
