LOCUS       sequence-182624-       16384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant 
            Selection: PvUbi2:hpt II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16384)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 16384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTaCas9-D10A_5
FEATURES             Location/Qualifiers
     source          1..16384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    4792..4813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4828..4833,4834..4851,4852..4858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4866..4882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4871..4893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4890..4906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4890..4906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(6963..6989,6990..6992,6993..11066)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11067..11114
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      11125..11373
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(11395..11412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11395..11411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11404..11426)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    11614..11638
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12939..13568
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13997..15070
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15136..15330
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(15566..15588)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15674..15814
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15729..15748
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15829..15846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(16000..16384,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16080..16099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga
     2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag
     2581 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc
     2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     2761 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg
     2821 acagacgtcg cggtgagttc aggctttttc ataagcttct gcaaaagaga accagacaac
     2881 agggtaagtg cctagcagta aacaaacaga actcatcaca agcaaacagc aacatcatat
     2941 tcataccaac aggtcatgtg tgttcatcac atcattagta ctaagcatgc catcatccaa
     3001 gtatatcaaa gtaagggcaa agagcatgca tgatcatcag gtgcacaaaa gaatcatcaa
     3061 attgtagcag tacatatctt catctatcat gcatatctat ccataacagg acgatgcatg
     3121 ttgaccaggt aaaagctaca ggatcctata ggaacagcag cgtatatatc ttcaccaatc
     3181 ttagcatctt aatcatgtgg cacatgcagt ttcaatttaa gcacatgagc tagttgatta
     3241 tgaggtacca gagaatcatc aaactgatgt agcagcatat atcctcatct atcatgcaat
     3301 ctaatctaat ctaactaaac aggaaaggtg tgctattcag ttaaaagcta ccgcatcata
     3361 tacaaacggc agcataagaa aaagcataat catcttaatc atgcaacaaa cgcagattca
     3421 taataagccc aagagctagc ttgtgatgat cttattctac tctgatctac agcaatcaga
     3481 taacgaccta accttgcaca tggcaacaaa acaatcgatc ggacgaatca gttgtttgtt
     3541 cctagctagc accatcgaac cagataatag atgcacgtac agatcccgaa aacgaaccca
     3601 aaaacagggc agacctagct gaacctaggc agcgacccag cagatcgtga gaacgatctc
     3661 atctacgaac agcctagaag caaccccacg attcccggac aaacgaccta aaatccccac
     3721 aaatcacatg agcatgacag gataaacagc ggaaccgatc agatctacac gaaaacccca
     3781 cctcccagcc acccacgatc aggaaacacg cggatctagc atgatttcgt caacgcctca
     3841 gcctagttcc tagccacaga ccaagcagaa ccaccaaacc acgccgagcg aggagatggg
     3901 gcaagaggac gggggagacg atcgccgtac cttgaagcgg gggaaggatc gccgagggtc
     3961 gcgaggagag caatttggat ttggaacccg ggggttgtgc gctccgaacg atgagacgat
     4021 gtgagattgt gggaagaggc gcggagggcc ctgtatttat gggctgcgac ggggggagga
     4081 gaggtgggga gggttgggga aggaatcccc cacccgtgcc gtgacggttc cgggccgtgt
     4141 gagaggagcc cgctcgtctc cgccacgcaa tttccgcgat cggagcggag ctttcgagag
     4201 gcggctggat ggttggtggc cgttagattt gtagacgccg ttaacgcctc gcctccaccg
     4261 ggaagagttt tgagcagccg cttatgacaa tggcttaacg acgttagacg gagcgttagt
     4321 ggcaggccat gatagaatag acgtattgca atgggattat aattaaataa ataagaatat
     4381 aataagatat ggcaagtcgg cactcatgac atggtcttcg aaatgatagt gctcactttc
     4441 ttagccgaga aagttgacgc gactgattta gaagttaaga tattatttct ctcttctttt
     4501 ctttcctcgt catataagga tgaaataaac tttagagatt gccggtagtg attttggatt
     4561 tcggcgatca ggcttggttt gccggtttcg gacggtgtgc cttaggccac ccgcagtgta
     4621 tcttgtaatg ttcaaccgat aagcaagggt ggggctcaag caagtagtaa acaactatgt
     4681 caaatgtcac catggttatg gtcttgttta gttggcttct tggccgattc attaatgcag
     4741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
     4921 gagctcggta cccggggatc tgcaggcgcg cctgcagtgc agcgtgaccc ggtcgtgccc
     4981 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     5041 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     5101 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     5161 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     5221 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     5281 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     5341 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     5401 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     5461 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     5521 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     5581 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     5641 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     5701 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     5761 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
     5821 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     5881 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     5941 cgctcgtcct cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt
     6001 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc
     6061 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac
     6121 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc
     6181 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc
     6241 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg
     6301 tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattaat tctgtttcaa
     6361 actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt
     6421 acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg
     6481 ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg
     6541 ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt
     6601 atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga
     6661 tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata
     6721 tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat
     6781 aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc
     6841 agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact
     6901 gtttcttttg tcgatgctca ccctgttgtt tggtgttact tcctgcaggg cgatctattc
     6961 gaatggacaa gaagtactcg atcggcctcg ccatcgggac gaactcagtt ggctgggccg
     7021 tgatcaccga cgagtacaag gtgccctcta agaagttcaa ggtcctgggg aacaccgacc
     7081 gccattccat caagaagaac ctcatcggcg ctctcctgtt cgacagcggg gagaccgctg
     7141 aggctacgag gctcaagaga accgctaggc gccggtacac gagaaggaag aacaggatct
     7201 gctacctcca agagattttc tccaacgaga tggccaaggt tgacgattca ttcttccacc
     7261 gcctggagga gtctttcctc gtggaggagg ataagaagca cgagcggcat cccatcttcg
     7321 gcaacatcgt ggacgaggtt gcctaccacg agaagtaccc tacgatctac catctgcgga
     7381 agaagctcgt ggactccacc gataaggcgg acctcagact gatctacctc gctctggccc
     7441 acatgatcaa gttccgcggc catttcctga tcgaggggga tctcaaccca gacaacagcg
     7501 atgttgacaa gctgttcatc caactcgtgc agacctacaa ccaactcttc gaggagaacc
     7561 cgatcaacgc ctctggcgtg gacgcgaagg ctatcctgtc cgcgaggctc tcgaagtcca
     7621 ggaggctgga gaacctgatc gctcagctcc caggcgagaa gaagaacggc ctgttcggga
     7681 acctcatcgc tctcagcctg gggctcaccc cgaacttcaa gtcgaacttc gatctcgctg
     7741 aggacgccaa gctgcaactc tccaaggaca cctacgacga tgacctcgat aacctcctgg
     7801 cccagatcgg cgatcaatac gcggacctgt tcctcgctgc caagaacctg tcggacgcca
     7861 tcctcctgtc agatatcctc cgcgtgaaca ccgagatcac gaaggctcca ctctctgcct
     7921 ccatgatcaa gcgctacgac gagcaccatc aggatctgac cctcctgaag gcgctggtcc
     7981 gccaacagct cccggagaag tacaaggaga ttttcttcga tcagtcgaag aacggctacg
     8041 ctgggtacat cgacggcggg gcctcacaag aggagttcta caagttcatc aagccaatcc
     8101 tggagaagat ggacggcacg gaggagctcc tggtgaagct caacagggag gacctcctgc
     8161 ggaagcagag aaccttcgat aacggcagca tcccccacca aatccatctc ggggagctgc
     8221 acgccatcct gagaaggcaa gaggacttct accctttcct caaggataac cgggagaaga
     8281 tcgagaagat cctgaccttc agaatcccat actacgtcgg ccctctcgcg cgggggaact
     8341 caagattcgc ttggatgacc cgcaagtctg aggagaccat cacgccgtgg aacttcgagg
     8401 aggtggtgga caagggcgct agcgctcagt cgttcatcga gaggatgacc aacttcgaca
     8461 agaacctgcc caacgagaag gtgctcccta agcactcgct cctgtacgag tacttcaccg
     8521 tctacaacga gctcacgaag gtgaagtacg tcaccgaggg catgcgcaag ccagcgttcc
     8581 tgtccgggga gcagaagaag gctatcgtgg acctcctgtt caagaccaac cggaaggtca
     8641 cggttaagca actcaaggag gactacttca agaagatcga gtgcttcgat tcggtcgaga
     8701 tcagcggcgt tgaggaccgc ttcaacgcca gcctcgggac ctaccacgat ctcctgaaga
     8761 tcatcaagga taaggacttc ctggacaacg aggagaacga ggatatcctg gaggacatcg
     8821 tgctgaccct cacgctgttc gaggacaggg agatgatcga ggagcgcctg aagacgtacg
     8881 cccatctctt cgatgacaag gtcatgaagc aactcaagcg ccggagatac accggctggg
     8941 ggaggctgtc ccgcaagctc atcaacggca tccgggacaa gcagtccggg aagaccatcc
     9001 tcgacttcct caagagcgat ggcttcgcca acaggaactt catgcaactg atccacgatg
     9061 acagcctcac cttcaaggag gatatccaaa aggctcaagt gagcggccag ggggactcgc
     9121 tgcacgagca tatcgcgaac ctcgctggct cccccgcgat caagaagggc atcctccaga
     9181 ccgtgaaggt tgtggacgag ctcgtgaagg tcatgggccg gcacaagcct gagaacatcg
     9241 tcatcgagat ggccagagag aaccaaacca cgcagaaggg gcaaaagaac tctagggagc
     9301 gcatgaagcg catcgaggag ggcatcaagg agctggggtc ccaaatcctc aaggagcacc
     9361 cagtggagaa cacccaactg cagaacgaga agctctacct gtactacctc cagaacggca
     9421 gggatatgta cgtggaccaa gagctggata tcaaccgcct cagcgattac gacgtcgatc
     9481 atatcgttcc ccagtctttc ctgaaggatg actccatcga caacaaggtc ctcaccaggt
     9541 cggacaagaa ccgcggcaag tcagataacg ttccatctga ggaggtcgtt aagaagatga
     9601 agaactactg gaggcagctc ctgaacgcca agctgatcac gcaaaggaag ttcgacaacc
     9661 tcaccaaggc tgagagaggc gggctctcag agctggacaa ggccggcttc atcaagcggc
     9721 agctggtcga gaccagacaa atcacgaagc acgttgcgca aatcctcgac tctcggatga
     9781 acacgaagta cgatgagaac gacaagctga tcagggaggt taaggtgatc accctgaagt
     9841 ctaagctcgt ctccgacttc aggaaggatt tccagttcta caaggttcgc gagatcaaca
     9901 actaccacca tgcccatgac gcttacctca acgctgtggt cggcaccgct ctgatcaaga
     9961 agtacccaaa gctggagtcc gagttcgtgt acggggacta caaggtttac gatgtgcgca
    10021 agatgatcgc caagtcggag caagagatcg gcaaggctac cgccaagtac ttcttctact
    10081 caaacatcat gaacttcttc aagaccgaga tcacgctggc caacggcgag atccggaaga
    10141 gaccgctcat cgagaccaac ggcgagacgg gggagatcgt gtgggacaag ggcagggatt
    10201 tcgcgaccgt ccgcaaggtt ctctccatgc cccaggtgaa catcgtcaag aagaccgagg
    10261 tccaaacggg cgggttctca aaggagtcta tcctgcctaa gcggaacagc gacaagctca
    10321 tcgccagaaa gaaggactgg gacccaaaga agtacggcgg gttcgacagc cctaccgtgg
    10381 cctactcggt cctggttgtg gcgaaggttg agaagggcaa gtccaagaag ctcaagagcg
    10441 tgaaggagct cctggggatc accatcatgg agaggtccag cttcgagaag aacccaatcg
    10501 acttcctgga ggccaagggc tacaaggagg tgaagaagga cctgatcatc aagctcccga
    10561 agtactctct cttcgagctg gagaacggca ggaagagaat gctggcttcc gctggcgagc
    10621 tccagaaggg gaacgagctc gcgctgccaa gcaagtacgt gaacttcctc tacctggctt
    10681 cccactacga gaagctcaag ggcagcccgg aggacaacga gcaaaagcag ctgttcgtcg
    10741 agcagcacaa gcattacctc gacgagatca tcgagcaaat ctccgagttc agcaagcgcg
    10801 tgatcctcgc cgacgcgaac ctggataagg tcctctccgc ctacaacaag caccgggaca
    10861 agcccatcag agagcaagcg gagaacatca tccatctctt caccctgacg aacctcggcg
    10921 ctcctgctgc tttcaagtac ttcgacacca cgatcgatcg gaagagatac acctccacga
    10981 aggaggtcct ggacgcgacc ctcatccacc agtcgatcac cggcctgtac gagacgagga
    11041 tcgacctctc acaactcggc ggggataaga gacccgcagc aaccaagaag gcagggcaag
    11101 caaagaagaa gaagtgactc gagatatgaa gatgaagatg aaatatttgg tgtgtcaaat
    11161 aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg
    11221 gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca aatgtttcta
    11281 taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact gtatgtgcta
    11341 tggtatggac tatggaatat gattaaagat aaggagctcc ggtgacggac tggcactggc
    11401 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
    11461 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    11521 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt
    11581 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct
    11641 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
    11701 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt
    11761 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
    11821 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
    11881 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
    11941 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
    12001 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
    12061 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
    12121 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
    12181 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
    12241 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
    12301 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
    12361 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
    12421 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
    12481 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
    12541 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
    12601 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
    12661 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
    12721 ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
    12781 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
    12841 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
    12901 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
    12961 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
    13021 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
    13081 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
    13141 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
    13201 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
    13261 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
    13321 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
    13381 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
    13441 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
    13501 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
    13561 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
    13621 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
    13681 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
    13741 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
    13801 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
    13861 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
    13921 gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact ggaaccccca
    13981 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
    14041 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
    14101 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
    14161 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
    14221 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
    14281 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
    14341 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
    14401 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
    14461 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
    14521 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
    14581 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
    14641 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
    14701 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
    14761 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
    14821 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
    14881 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
    14941 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
    15001 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
    15061 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
    15121 aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
    15181 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
    15241 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
    15301 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag cccaagagct
    15361 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
    15421 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
    15481 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
    15541 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa acggtcacag
    15601 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    15661 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
    15721 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    15781 gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct cgctcactga
    15841 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    15901 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    15961 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    16021 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    16081 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    16141 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    16201 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    16261 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    16321 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    16381 gtat
//
