LOCUS       sequence-182678-       10374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the expression of NLS-tPMCP-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Shao S, Zhang W, Hu H, Xue B, Qin J, Sun C, Sun Y, Wei W, Sun Y
  TITLE     Long-term dual-color tracking of genomic loci by modified sgRNAs of 
            the CRISPR/Cas9 system.
  JOURNAL   Nucleic Acids Res. 2016 May 19;44(9):e86. doi: 10.1093/nar/gkw066. 
            Epub 2016 Feb 4.
  PUBMED    26850639
REFERENCE   2  (bases 1 to 10374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-05-19"; volume: "44(9):e86. doi"; pages: " 
            10.1093/nar/gkw066. Epub 2016 Feb 4"
COMMENT     Sequence Label: pHR-TRE3G-NLS-tdPCP-EGFP
FEATURES             Location/Qualifiers
     source          1..10374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2..18)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             108..341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(366..384)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(367..384)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(674..693)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(907..926)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1026..1048
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        1172..1276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1277..1345,1346..2137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1495..1514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2308..2896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2797..2816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3050..3067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3137..3157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3142..3471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3322..3457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4605..4670
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4800..4820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5245..5379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5282..5301)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5336..5355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5548..6181
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6228..6353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6850..7082
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7267..7311
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7596..7713
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7842..8156
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    7850..7868
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7886..7904
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7921..7939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7957..7975
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7993..8011
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8028..8046
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8064..8082
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8129..8153
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             8173..8193
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8197..8223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             8197..8223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8224..8235
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      8998..9007
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9004..9006,9007..9009,9010..9723)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9004..9006,9007..9009,9010..9720)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9049..9070)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9310..9329)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9657..9678
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9784..10372
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9837..9857)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10255..10266)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
       61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
      121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
      181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
      361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
      481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
      541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
      601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
      901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtctttct
     2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     3481 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     3541 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     3601 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     3661 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     3721 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     3781 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     3841 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     3901 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     3961 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     4021 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     4081 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     4141 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     4201 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     4261 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     4321 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     4381 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     4441 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     4501 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     4561 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     4621 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     4681 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     4741 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     4801 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     4861 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     4921 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     4981 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     5041 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     5101 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     5161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5221 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5341 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     5401 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctagtggtt
     5461 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     5521 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     5581 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     5641 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     5701 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     5761 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     5821 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     5881 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     5941 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     6001 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6061 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     6241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     6301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     6361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     6421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     6481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     6541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     6601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     6661 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     6721 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     6781 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     6841 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     6901 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     6961 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     7021 catcaagcag ctccaggcaa gaatcctggc tgtgaaagat acctaaagga tcaacagctc
     7081 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7141 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     7201 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     7261 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     7321 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     7381 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     7441 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     7501 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     7561 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     7621 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     7681 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     7741 ccagtttgga tcgataagct tgatatcgaa ttcgaaacca ttattatcat gacattaacc
     7801 tataaaaata ggcgtatcac gaggcccttt cgtcttcact cgagtttact ccctatcagt
     7861 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac
     7921 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
     7981 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga
     8041 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc
     8101 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     8161 acgcgtatgg gcccaaaaaa gaaaagaaaa gttggctacc cctacgacgt gcccgactac
     8221 gccatcgaag gccgccatat gctagcctcc aaaaccatcg ttctttcggt cggcgaggct
     8281 actcgcactc tgactgagat ccagtccacc gcagaccgtc agatcttcga agagaaggtc
     8341 gggcctctgg tgggtcggct gcgcctcacg gcttcgctcc gtcaaaacgg agccaagacc
     8401 gcgtatcgcg tcaacctaaa actggatcag gcggacgtcg ttgattccgg acttccgaaa
     8461 gtgcgctaca ctcaggtatg gtcgcacgac gtgacaatcg ttgcgaatag caccgaggcc
     8521 tcgcgcaaat cgttgtacga tttgaccaag tccctcgtcg cgacctcgca ggtcgaagat
     8581 cttgtcgtca accttgtgcc gctgggccgt gcggatccgc tagcctccaa aaccatcgtt
     8641 ctttcggtcg gcgaggctac tcgcactctg actgagatcc agtccaccgc agaccgtcag
     8701 atcttcgaag agaaggtcgg gcctctggtg ggtcggctgc gcctcacggc ttcgctccgt
     8761 caaaacggag ccaagaccgc gtatcgcgtc aacctaaaac tggatcaggc ggacgtcgtt
     8821 gattccggac ttccgaaagt gcgctacact caggtatggt cgcacgacgt gacaatcgtt
     8881 gcgaatagca ccgaggcctc gcgcaaatcg ttgtacgatt tgaccaagtc cctcgtcgcg
     8941 acctcgcagg tcgaagatct tgtcgtcaac cttgtgccgc tgggccgtcc accggtcgcc
     9001 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     9061 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     9121 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     9181 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     9241 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     9301 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     9361 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     9421 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     9481 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     9541 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     9601 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     9661 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     9721 taaagcggcc gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc
     9781 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     9841 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     9901 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     9961 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
    10021 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    10081 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    10141 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    10201 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    10261 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    10321 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcat
//
