LOCUS       sequence-18269-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ristow M, Pfister MF, Yee AJ, Schubert M, Michael L, Zhang CY, Ueki 
            K, Michael MD, Lowell BB, Kahn CR
  TITLE     Frataxin activates mitochondrial energy conversion and oxidative 
            phosphorylation.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Oct 24. 97(22):12239-43.
  PUBMED    11035806
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Oct 24. 97(22):12239-43."
COMMENT     Sequence Label: pBS human Frataxin (myc tag)
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(73..102)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(746..762)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(746..762)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(794..813)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(795..813)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggaacaaaa gctgggtacc gggctccctc ttgaggtcga cggtatcgat aagcttgata
       61 tcgaattccc taaaggtcct cctcgctaat tagcttttgc tcagcatctt ttccggaata
      121 ggccaaggaa gacaagtcca gtttggtttt taaggcttta gtgagctctg cggccagcag
      181 ctcatggagg gacacgccgt cgtgggagta cacccagttt ttcccagtcc agtcataaca
      241 cttaggtcca ctggatggag aagatagcca gatttgcttg tttggcgtct gcttgttgat
      301 cacataggtt cctggatctc cacccagttt gacagttaag acaccactcc caaaggagac
      361 atcatagtcc tcaaacgtgt atggcttgtc tgcaaggtct tcaaaaaact ctgctaaaga
      421 gtccagcgtt tcctctgcta gtctttcata ggtggtctca tctagagagc ctgggtggcc
      481 caaagttcca gatttcctca aattcatcaa atagacactc tgctttttga cattccaaat
      541 ctggttgagg ccacgttggt tcgaacttgc gcggcggggc gtgcaggtcg catcgatgtc
      601 ggtgcgcagg ccacggcggc cgcagagtgg ggccaactct gccggccgcg ggacccgggt
      661 gagggtctgg gcctgggccg ggctgggtga cgccaggagg ccggctactg cgcggcgccc
      721 gagagtccac atgctgctcc ggcgggatcc actagttcta gagcggccgc caccgcggtg
      781 gagctccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttt
//
