LOCUS       sequence-182697-        4656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry TOPO TA cloning vector ELF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label: pCR8/GW/TOPO-ELF1
FEATURES             Location/Qualifiers
     source          1..4656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1999..2098)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2115..2134)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2116..2134)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2139..2155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2139..2155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2586..3377
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3470..4058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3959..3978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4212..4229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4388..4415)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4507..4593)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4642..4656,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4656,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccgaattc gcccttacca tggctgctgt tgtccaacag aacgacctag
      181 tgtttgaatt tgcaagtaac ggcatggagg acgagcagca gcttggtgat cctgctatat
      241 ttcctgctgt tattgtggaa catgttcctg gtgctgatat tctcaatagt tatgctggtc
      301 tagcttgtgt ggaagagcct aatgacatga ttactgagag ctcattggat gttgctgaag
      361 aagaaatcat agacgatgac gatgacgaca tcacccttac agttgaggct tcttgtcata
      421 atggagatga aacaattgag accattgagg ctgccgaggc actcctcaat atagattctc
      481 ccagccctcc tgtactggat gaaaagcaaa tcaataataa tatatttagc tcctctgaag
      541 atgacatcgt tgccccaatc acccatgtgt ccgtcacatt agatgggatt cctgaagtga
      601 tggaaactca gcaagtgcaa gagacaaatg cagactcccc aggagcctcc tccccagagc
      661 agcgcaagag aaagaaagga aggaaaacaa aaccaccacg gccagattcc ccgacgacta
      721 caccaaatat ctctgtgaaa aagaaaaata aagatgggaa gggaaacaca atttaccttt
      781 gggagttttt gctggccctg cttcaggaca aagctacctg tcctaagtac atcaagtgga
      841 cgcagcgaga aaagggcatt tttaagctgg ttgattctaa agccgtgtct agattgtggg
      901 ggaagcacaa aaacaaacct gacatgaact acgagaccat ggggagagca ctcaggtact
      961 attaccaaag aggtattctt gcaaaagtgg aaggtcagcg cttggtgtat cagtttaaag
     1021 aaatgccaaa agatcttatc tacatagatg atgaggatcc aagttccagc atagagtctt
     1081 cggatcagtc cttatcgtca acaaccgcat caagtcggaa tcaagcaaac cgatcaagag
     1141 tatcttcaag tccaggaatt aaaggagggg ccgctacaat tctaaagccc gggaattcta
     1201 aagctgcaaa ccccaaagat ccggtggaag ttggacaacc ttcagaagtt ctgaggacag
     1261 tgcaaccctc acaggctccc tatcctaccc agctcttccg aactgttcat gtagtacagc
     1321 cagtacaagc tgtcccagag gaagcaacca tagccagcac catgcaggag gaagcagcaa
     1381 attcttcagt tccaagtatt aggactatac aggcttcaac ccaggttcca gtagtggtgt
     1441 ctcctggcaa ccaacagtta catacagtaa cagtgccact cacaacggtt atagccagca
     1501 tagacccatc atcaggtgct gggtctcaga aattcatttt acaaaccatt ccatcgtcac
     1561 agcccatgac agtactgaaa gaaaatgtca tgctacagtc acagaagcca ggctctcctt
     1621 caattgtcct cagccccacc caagtacagc aggtcctcac aagcaatgtt cagtccattt
     1681 gcaatggagc gggcagtgtg gcatccgcac catcgttcag cgcgacgaca cctgtggtga
     1741 ctttctctcg gagttcacag ctggtcgcac acccaccggg cactgtgatc acttctgtta
     1801 tcaaagctca ggaaacaaaa actcttaagc aagaggtgga gaaaaaggcc gaagatgatt
     1861 tgaatgaaga tgctgagaaa agtgcccagc agccccagcc ttatgtgatg gtgttatcca
     1921 gttcaaacgg gttttcctct caggtagccg tcaaacagaa tgaactgcta gagcccaact
     1981 cttttaaggg cgaattcgac ccagctttct tgtacaaagt tggcattata aaaaataatt
     2041 gctcatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     2101 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2161 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2221 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     2281 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     2341 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     2401 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     2461 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2521 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2581 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2641 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2701 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2761 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2821 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2881 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2941 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     3001 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3061 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3121 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3181 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3241 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3301 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3361 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     3421 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3481 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3541 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3601 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3661 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3721 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3781 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3841 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3901 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3961 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4021 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4081 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4141 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4201 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4261 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     4321 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     4381 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     4441 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     4501 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4561 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     4621 taacgctagc atggatgttt tcccagtcac gacgtt
//
