LOCUS       sequence-182699-        4107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry TOPO TA cloning vector ELK1 Y158A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label: pCR8/GW/TOPO-ELK1Y158A
FEATURES             Location/Qualifiers
     source          1..4107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1450..1549)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1566..1585)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1567..1585)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1590..1606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1590..1606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2037..2828
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2921..3509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3410..3429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3663..3680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3839..3866)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3958..4044)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4093..4107,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4107,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccgaattc gcccttacca tggacccatc tgtgacgctg tggcagtttc
      181 tgctgcagct tctgagagaa caaggtaatg gccacatcat ctcctggacc tcacgggatg
      241 gtggtgagtt caagttggtg gatgcagagg aggtggcccg gctatgggga ctgcgcaaga
      301 acaagaccaa catgaattac gacaagctta gccgggcctt gcggtactac tatgataaga
      361 atatcatccg caaggtgagc ggccagaagt ttgtctacaa gtttgtgtcc tacccagagg
      421 ttgcagggtg ctccactgaa gactgcccac cccagcctga ggtgtctgta acctcggcca
      481 tagccatggc ccctgctact gtccatgcag gcccagggga cactgccact ggaaagccag
      541 gaacaccaaa gggtgcagga atgacaggcc aaggtggctt agcacgaagc agccggaatg
      601 aatacatgcg ctcgggcctc gcgtctacct tcacaataca atccctgcag ccacagccac
      661 agccacccat tcctcctcgg cctgcctcag tgcttcccaa cactacccct gcaggagtac
      721 cagcacccgc ctcagggagc aggagcacca gtccaaaccc cttagaagcc tgtttggaag
      781 cagaagaggc tggtctgccc ctgcaggtta tcctaacccc accagaggcc ccaaaccaga
      841 aatccgaaga gttgagtctg gacccaagtt ttggccatcc acagccccca gaagtcaaag
      901 tggaggggcc taaggaagaa ttggaagctg caagggctgg aggcttcagt tcagaagctg
      961 tcaaagctga accagaagtc tcagcctcag aaggcctgct ggctcggctc ccagccatcc
     1021 taacagagaa cacagcccag gtgtgtggcc tctccacttc caccactgag atcacccaac
     1081 cgcagaaggg ccgaaagcct cgggacctgg aacttccact tagcccaagc ctgctgggtg
     1141 gccagggacc tgaacggact ccaggatcag gaacaagctc tggtcttcag gcaccggggc
     1201 cagcgctaac gccatccctg ctccccacac ataccttgac cccggtgctg ctgacaccca
     1261 gctcgctgcc ccctagcatc catttctgga gcactctgag tccaattgca ccccgtagtc
     1321 cagccaagct ctccttccag tttccgtcca gtggcagcgc acaggtgcac atcccttcca
     1381 tcagtgtgga tggcctctcg acccccgtgg tgctctcccc agggccccag aagccaaagg
     1441 gcgaattcga cccagctttc ttgtacaaag ttggcattat aaaaaataat tgctcatcaa
     1501 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     1561 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     1621 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
     1681 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     1741 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     1801 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1861 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     1921 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     1981 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
     2041 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     2101 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     2161 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     2221 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     2281 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     2341 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     2401 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     2461 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     2521 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     2581 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     2641 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     2701 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     2761 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     2821 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2881 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2941 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3001 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3061 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3121 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3181 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3241 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3301 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3361 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3421 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3481 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3541 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3601 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3661 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3721 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3781 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     3841 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     3901 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     3961 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     4021 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
     4081 catggatgtt ttcccagtca cgacgtt
//
