LOCUS       sequence-182709-        8282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of membrane green-to-red photoconvertible 
            Dendra2 and the nuclear blue fluorescent protein Cerulean..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Dempsey WP, Fraser SE, Pantazis P
  TITLE     PhOTO zebrafish: a transgenic resource for in vivo lineage tracing 
            during development and regeneration.
  JOURNAL   PLoS One. 2012;7(3):e32888. doi: 10.1371/journal.pone.0032888. Epub 
            2012 Mar 14.
  PUBMED    22431986
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e32888. doi"; pages: " 
            10.1371/journal.pone.0032888. Epub 2012 Mar 14"
COMMENT     Sequence Label: PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)
FEATURES             Location/Qualifiers
     source          1..8282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     137..156
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     396..417
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      459..468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(481..858)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             complement(865..918)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(964..1653)
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     regulatory      1707..1716
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1806..1824)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1807..1824)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(4539..4726,4727..4738)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4772..4788)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4772..4788)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4825..4845)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4825..4843)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4864..4880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4864..4880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4877..4899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4888..4904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4912..4918,4919..4936,4937..4942))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4957..4978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5095..5112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5266..5854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5346..5365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6025..6816,6817..6885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6648..6667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6886..6990)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7153..7174)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7365..7384
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7528..7550
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7542..7559
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7543..7559
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7569..7588
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7569..7587
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(7618..7629,7630..7767)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    7859..7993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7896..7915)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7950..7969
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8000..8018
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(join(8025..8282,1..456,457..459,460..462))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8070..8091)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtgatatag acgttgtcgc tgatggcgtt gtactccagc ttgtgcccca ggatgttgcc
       61 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      121 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      181 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      241 gcgggcgaag cactgcacgc cccaggtcag ggtggtcacg agggtgggcc agggcacggg
      301 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      361 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      421 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga cctgtggatc
      481 cttagcgctg gtgtacttgg tgatggcctt agtaccctcg gacacggcgt gcttggccaa
      541 ctccccaggc agcagcaggc gcacggccgt ctggatctcc ctggaggtga tggtcgagcg
      601 cttgttgtaa tgcgccaggc gggaagcctc acctgcgatg cgctcgaaaa tgtcgttcac
      661 aaacgaattc atgatgccca tggccttgga cgaaatgccg gtgtcagggt ggacctgctt
      721 cagaaccttg tacacataga tggaatagct ctccttgcgg ctgcgcttgc gcttcttgcc
      781 gcctttcttc tgcgccttag tcaccgcctt cttggagccc tttttcgggg cgggagcaga
      841 cttcgctggc tctggcatta cgtaagggcc gggattctcc tccacgtcac cgcatgttag
      901 aagacttcct ctgccctctc ctgatccctt aattaaactc ttgtacacgc cgctgtcgcc
      961 ggaccacacc tggctgggca gggggctgta gcgggccacg gcgtgctcgt acagcttcac
     1021 cttgttgtag tcgctgtcgt tgcccaggat ctcgatgcgg tggtccacga agtgggcgtc
     1081 gggcagctgc accaccttct tggccttgta ggtggtcttg aagtcgcaca ggtagtggcc
     1141 gccgccctcc agcaacaggg ccatgttgat gttgcccacc agcaggccgt cgcgcacgtg
     1201 cagcttctcg gtgctgggct cccacttcag ggtcttcttc tgcatcacgg ggccgttggg
     1261 ggggaagttg gtgcccttga agcgcacgtt ctggaagaag cagtcgccct ccaggctgat
     1321 gtcgctgcgg atggtgcaga tgcccttgtc ctcgaaggtc atggtgcgct cccagctgta
     1381 gccctcgggg aagctctgct tgaagtagtc ggggatgtcc tcggggtact tggtgaacac
     1441 ccggttgccg tagtgcacgg cggtggtcag gatgtcgtag ctgaagggca ggggggcgcc
     1501 ctccttcacg gtcaggttgg cggtctgggt gccctcgtag ggcttgccct tgccctcgcc
     1561 ctcgatcacg aaggcgtggc cgttcacgtt gccctccatg tgcaccttca cgcgcatgtc
     1621 ctccttgatc aggttaattc ccggggtgtt catgaccggt gcctcgtcgt cgttcaggtt
     1681 gtccttgcgc ttgctcttga tgcagcccat ggcggcctcg tcgtcgttca ggttgtcctt
     1741 gcgcttgctc ttgatgcagc ccatatcgat gggatcctgc aaaaagaaca agtagcttgt
     1801 actcttctat agtgtcacct aaataggcct aggatggcga actgtaaaag aaagggaaac
     1861 tgttagaatt gcactgctgc cagggagagg cgtttcagca agcatgtgac catctggagt
     1921 caacttcctg ttttgctctg cattctacag tctgaccgct ggtgggcgct agtgacagcg
     1981 cgcgcctcag atatgccaga caaaggaagt ccctctgcat tctctcatta ttaccataaa
     2041 aggcaatggt ttgagccgct ttgcggctgc cggcggtgtg gcactcagcc agagtcgtag
     2101 ggctcagtaa aaaggaacag atttcgacat tcatcacacc aggaccactc cctaaatttt
     2161 acacaaatga agtttcacat cctcttccac cctcgcctgc gatcaattac acaaccgcat
     2221 ttcaacgggt gtctgccgcc gggtgttact gcgtcacaga tctgcagtca gtccacggac
     2281 tcctcgtgag cggagacttc acactaaagc atttctggac ggtcactctc agtcggttca
     2341 gttcatagtc tagcctcaaa aatcaccgag atcctgaaaa actgatgggc tctgcttcga
     2401 cagtcacagc ggctgaagtg cagccagaga actccgcagc cgttattaaa gtggaccaca
     2461 cctctaaaat tcaaatcttt catagcggat acaacacttc actaacgtta acgttaatct
     2521 tcagcttcat taaagacaga gggggctgca ggctacattt aaatagaaca cataacaaaa
     2581 agaaaattaa ccaagttaaa actaaatgcc aatacccgtt actaataaca agatcttcga
     2641 tcttataaag cacgaatgag ccgatgttag attgatgggg tttacgttac atcagatgag
     2701 agctggttag ccggctagct aaagtaggtc acaccctgct gcagcactgc ctgcacaaag
     2761 aacttcccat gccggctatc catcaagtgt gcaacatgct caccagcacc ctcaaaactg
     2821 tagttaaaaa tcacatttat ggctgttaaa gagagattag ccagaacaca actttatatt
     2881 aagacttaac acccatttca caccggccta ttcaatgttc aatgtcgtgc ctacgactgg
     2941 tcacgttaaa ttaccgacat tctcacctgc cacatccact aacgctacaa aaaagttaag
     3001 atacagccaa tatttattaa atataacata gattatcaaa agaaccacca cttataaatc
     3061 aaacctagta gataactaca aagagaccgt tatgaactgt ctaaagactt catcaaatca
     3121 tatttatcca tttacacatt ttacttaaca ttaatagata gaaactataa ttaaacgctt
     3181 aaaaactgta aaaccaactc accttgggtt tggggggttg tcgacttaag agaatgggtt
     3241 acaagcagat gatattccgc actagctgcg tgtggaggag ctcaaagtga ggctgaaaag
     3301 ctgggcgcgc tcttttatat gacggcacat acccggctct agccataaaa ggtaaacttt
     3361 cggagctctg tgctctgatt ggtccagcgt cacacacccg gctgtcgcgt gcacattgct
     3421 acacttgctt cttgcaactt gagagtgaaa gaaggggaag cttaactggt taaagaacaa
     3481 ggccatggcg atttcctata aaactggaat ttttatttgc aaattttaac acagaatttt
     3541 tcgtattttt ctgcgacaac gccaagactc aattaaactt ttcaaaaagt ctgtgcagag
     3601 ttacaagtgg cagaatgcgt cataatgatg gatgcctcca tgatcctgtg ttgtgttttc
     3661 aatctaacac aacagcagct ctgtaggtca tttacctctt gtgttcacga aataccgtaa
     3721 tacaaacaat gggggatcta atgagtactt ttccatgtat aaaatggaca ttttttttat
     3781 ccaaattagg atgacactac ttgtagtatg ttgtatgagc aactagctgg tcttagctca
     3841 gtagtaatgc actttaagaa agtgctttaa taactttgaa tgaatgggac ctgcattcca
     3901 tgccacattg cttcactatt tttgtgtgta caagttacac aatgatgtat aaagtgagtg
     3961 atagcagtta catttttgca agtgggccta cctcaggttt aatgttcatt agaaatgtta
     4021 tatgtcaagt cttgatctta aatagttaaa ttttctgttc ataatttcat gtcgtgtcta
     4081 tgcagacata acatttcttt ttaaagaggt ttataaggat aaatcctaag aacataattc
     4141 tcattaaaag tagaaaaaaa aaaacactga aagtgttggt gatcagctac aataaataat
     4201 attttgcaca catttcagat attgccacac tgaataacag ataaagctgt gcttgagcag
     4261 cagtgatcat gataaactac atgtgacttt ttactgtgta acttgggagt aatgcacatg
     4321 gccttcagtg ggagttataa acaaaaacag ctgattgtgt taaaagtgct tgctgatacc
     4381 aaggacaatg aggtgatgtg acttttattt tgtttttggc aaattccaag tattttgaca
     4441 agacttttta caattctgag cattttacct ctaaaatatg gaagtttctc agagtaaacg
     4501 ttgagtatag gtaatgacta ggccctcgag ccgggcccaa gtgatctcca aaaaataagt
     4561 actttttgac tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg
     4621 taattaagta aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc
     4681 aaaaataata cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt
     4741 tcttgacccc ctaccttcag caagcccagc agatccacta gttctagagc ggccgccacc
     4801 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     4861 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4921 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4981 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5041 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5101 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5161 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5221 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5281 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5341 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5401 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5461 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5521 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5581 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5641 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5701 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5761 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5821 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5881 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5941 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6001 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6061 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6121 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6181 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6241 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6301 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6361 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6421 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6481 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6541 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6601 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6661 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6721 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6781 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6841 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6901 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6961 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     7021 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     7081 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     7141 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     7201 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     7261 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     7321 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     7381 ggagcgggcg ctagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     7441 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     7501 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     7561 agcgcgcgta atacgactca ctatagggcg aattgggtac cggcatatgg ttcttgacag
     7621 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
     7681 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
     7741 actccattaa aattgtactt gagtattaga tctgcatgcg tttaaacccg ggatatcaaa
     7801 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     7861 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7921 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7981 tcatgtctgg atcaccggtg ctagttattg ctcagcggga cgtcttactt gtacagctcg
     8041 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     8101 ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt gtcgggcagc
     8161 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     8221 ccgtcctcga tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg
     8281 gc
//
