LOCUS       sequence-182716-       16512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV T-DNA vector expressing TALENs targeting tomato ANT1, 
            selectable marker gene removed from the backbone (template for 
            amplification of the BaeI containing fragment to create pMOD_C0000).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16512)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 16512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC130
FEATURES             Location/Qualifiers
     source          1..16512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        880..1225
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1168..1188
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1259..1324
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1331..1351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4685..4741
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4745..4810
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4817..4837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7568..8155
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      8169..8417
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    10138..10162
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11463..12092
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12521..13594
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13660..13854
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14090..14112)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14198..14338
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14253..14272
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14353..14370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14524..15112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14604..14623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15199..15993)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    16418..16442
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agctcaaagt ttaacgcgtt agcagaaggc atgttgttgt gactccgagg ggttgcctca
       61 aactctatct tataaccggc gtggaggcat ggaggcaggg gtattttggt cattttaata
      121 gatagtggaa aatgacgtgg aatttactta aagacgaagt ctttgcgaca agggggggcc
      181 cacgccgaat ttaatattac cggcgtggcc cccccttatc gcgagtgctt tagcacgagc
      241 ggtccagatt taaagtagaa aatttcccgc ccactagggt taaaggtgtt cacactataa
      301 aagcatatac gatgtgatgg tatttgatgg agcgtatatt gtatcaggta tttccgttgg
      361 atacgaatta ttcgtacgac cctcggcgcg ccagatttgc cttttcaatt tcagaaagaa
      421 tgctaaccca cagatggtta gagaggctta cgcagcaggt atcatcaaga cgatctaccc
      481 gagcaataat ctccaggaaa tcaaatacct tcccaagaag gttaaagatg cagtcaaaag
      541 attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac
      601 tattccagta tggacgattc aaggcttgct tcacaaacca aggcaagtaa tagagattgg
      661 agtctctaaa aaggtagttc ccactgaatc aaaggccatg gagtcaaaga ttcaaataga
      721 ggacctaaca gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact
      781 caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa
      841 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt
      901 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
      961 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
     1021 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     1081 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
     1141 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     1201 aagttcattt catttggaga gaacacgggg gactcctgca ggaaaaatgg cttcctccga
     1261 ttataaggat cacgatggcg actacaagga ccacgatatt gactacaaag acgatgacga
     1321 taaaatggct cctaagaaaa agcgcaaagt cggtatccat ggcgttccct ctagaatggt
     1381 ggatctacgc acgctcggct acagtcagca gcagcaagag aagatcaaac cgaaggtgcg
     1441 ttcgacagtg gcgcagcacc acgaggcact ggtgggccat gggtttacac acgcgcacat
     1501 cgttgcgctc agccaacacc cggcagcgtt agggaccgtc gctgtcacgt atcagcacat
     1561 aatcacggcg ttgccagagg cgacacacga agacatcgtt ggcgtcggca aacagtggtc
     1621 cggcgcacgc gccctggagg ccttgctcac ggatgcgggg gagttgagag gtccgccgtt
     1681 acagttggac acaggccaac ttgtgaagat tgcaaaacgt ggcggcgtga ccgcaatgga
     1741 ggcagtgcat gccagcagga atgcgttaac aggtgccccc ctgaacctga ccccggacca
     1801 agtggtggct atcgccagca acattggcgg caagcaagcg ctcgaaacgg tgcagcggct
     1861 gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag
     1921 caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     1981 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca
     2041 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc
     2101 ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca
     2161 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     2221 cgccagcaac attggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     2281 gtgccaggac catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg
     2341 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     2401 gaccccggac caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac
     2461 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
     2521 ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     2581 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg
     2641 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     2701 tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct
     2761 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     2821 agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct
     2881 gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag
     2941 caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     3001 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca
     3061 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc
     3121 ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca
     3181 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     3241 cgccagcaac attggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     3301 gtgccaggac catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg
     3361 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     3421 gaccccggac caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac
     3481 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt
     3541 ggctatcgcc agccacgatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     3601 ggtgctgtgc caggaccatg gcctgactcc ggaccaagtg gtggctatcg ccagccacga
     3661 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     3721 tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct
     3781 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     3841 agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaagca ttgtggccca
     3901 gctgagccgg cctgatccgg cgttggccgc gttgaccaac gaccacctcg tcgccttggc
     3961 ctgcctcggc ggacgtcctg ccatggatgc agtgaaaaag ggattgccgc acgcgccgga
     4021 attgatcaga agagtcaatc gccgtattgg cgaacgcacg tcccatcgcg ttgccggatc
     4081 ccagctggtg aagtccgagc tggaagaaaa aaagagcgag ctgcgccaca agctcaagta
     4141 cgtgccccac gagtacatcg agctgatcga gatcgcccgc aacagcaccc aagaccgcat
     4201 cctggagatg aaagtgatgg agttcttcat gaaggtgtac ggctaccgcg gcaagcacct
     4261 gggcggctcc cgcaagcccg atggcgccat ctacaccgtg ggctccccca tcgactatgg
     4321 cgtcattgtc gacaccaagg cctactccgg cggctacaac ttacccatcg gtcaggccga
     4381 cgagatgcaa cgctacgtga aggagaacca gacccgcaat aagcacatta atcccaacga
     4441 gtggtggaag gtgtacccct cctccgtgac cgagttcaaa ttcctgttcg tgtccggcca
     4501 cttcaagggc aattataagg cccaactgac ccgcctgaac cgcaagacca actgcaacgg
     4561 cgccgtgctg tccgtggagg aactgctgat cggcggcgag atgatcaagg ctggtaccct
     4621 gaccctggaa gaggtgcgcc gcaagttcaa caatggtgaa atcaatttca ggtccggaag
     4681 cggcgccacg aacttctctc tgttaaagca agcaggtgac gtggaagaaa acccagggcc
     4741 catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
     4801 cgatgacaag atggccccca agaagaagag gaaggtgggc attcacgggg tgccggctag
     4861 catggtggat ctacgcacgc tcggctacag tcagcagcag caagagaaga tcaaaccgaa
     4921 ggtgcgttcg acagtggcgc agcaccacga ggcactggtg ggccatgggt ttacacacgc
     4981 gcacatcgtt gcgctcagcc aacacccggc agcgttaggg accgtcgctg tcacgtatca
     5041 gcacataatc acggcgttgc cagaggcgac acacgaagac atcgttggcg tcggcaaaca
     5101 gtggtccggc gcacgcgccc tggaggcctt gctcacggat gcgggggagt tgagaggtcc
     5161 gccgttacag ttggacacag gccaacttgt gaagattgca aaacgtggcg gcgtgaccgc
     5221 aatggaggca gtgcatgcat cgcgcaatgc actgacgggt gcccccctga acctgacccc
     5281 ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca
     5341 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     5401 cgccagcaac ggtggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     5461 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca acggtggcgg
     5521 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     5581 gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaac
     5641 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
     5701 ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     5761 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg
     5821 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     5881 tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct
     5941 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     6001 agtggtggct atcgccagca acaatggcgg caagcaagcg ctcgaaacgg tgcagcggct
     6061 gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag
     6121 caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     6181 ggaccatggc ctgactccgg accaagtggt ggctatcgcc agccacgatg gcggcaagca
     6241 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc
     6301 ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca
     6361 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     6421 cgccagcaac attggcggca agcaagcgct cgaaacggtg cagcggctgt ttccggtrct
     6481 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca acggtggcgg
     6541 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     6601 gaccccggac caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac
     6661 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
     6721 ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     6781 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg
     6841 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     6901 tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct
     6961 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     7021 agtggtggct atcgccagca acattggcgg caagcaagcg ctcgaaacgg tgcagcggct
     7081 gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag
     7141 ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     7201 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca
     7261 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc
     7321 ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaagcattgt
     7381 ggcccagctg agccggcctg atccggcgtt ggccgcgttg accaacgatc atctggtggc
     7441 actcgcgtgc ctcggcggac gtcctgccat ggatgcagtg aaaaagggat tgccgcacgc
     7501 gccggaattg atcagaagag tcaatcgccg tattggcgaa cgcacgtccc atcgcgttgc
     7561 cagatctcaa ctagtcaaaa gtgaactgga ggagaagaaa tctgaacttc gtcataaatt
     7621 gaaatatgtg cctcatgaat atattgaatt aattgaaatt gccagaaatt ccactcagga
     7681 tagaattctt gaaatgaagg taatggaatt ttttatgaaa gtttatggat atagaggtaa
     7741 acatttgggt ggatcaagga aaccggacgg agcaatttat actgtcggat ctcctattga
     7801 ttacggtgtg atcgtggata ctaaagctta tagcggaggt tataatctgc caattggcca
     7861 agcagatgaa atggagcgat atgtcgaaga aaatcaaaca cgagacaaac atctcaaccc
     7921 taatgaatgg tggaaagtct atccatcttc tgtaacggaa tttaagtttt tatttgtgag
     7981 tggtcacttt aaaggaaact acaaagctca gcttacacga ttaaatcata tcactaattg
     8041 taatggagct gttcttagtg tagaagagct tttaattggt ggagaaatga ttaaagccgg
     8101 cacattaacc ttagaggaag tgaggcggaa atttaataac ggcgagataa acttttaata
     8161 gctcgagata tgaagatgaa gatgaaatat ttggtgtgtc aaataaaaag cttgtgtgct
     8221 taagtttgtg tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta
     8281 aatgaatgta agatcacatt ataatgaata aacaaatgtt tctataatcc attgtgaatg
     8341 ttttgttgga tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga
     8401 atatgattaa agataaggag ctttgggtac gtcacgtggc tcgagcgcgt agtcctcggt
     8461 aatatcgcag aacaaaagta cctgatatcg agtgtacttc aagtcagtgg gaaatcaata
     8521 aaatgattat tttatgaata tatttcattg tgcaagtaga tagaaattac atatgttaca
     8581 taacacacga aataaacaaa aaaagacaat ccaaaaacaa acaccccaaa aaaaataatc
     8641 actttagata aactcgtatg aggagaggca cgttcagtga ctcgacgatt cccgagcaaa
     8701 aaaagtctcc ccgtcacaca tgtagtgggt gacgcaatta tctttaaagt aatccttctg
     8761 ttgacttgtc attgataaca tccagtcttc gtcaggattg caaagaatta tagaagggat
     8821 cccacctttt attttcttct tttttccata tttagggttg acagtgaaat cagactggca
     8881 acctattaat tgcttccaca atgggacgaa cttgaagggg atgtcgtcga tgatattata
     8941 ggtggcgtgt tcatcgtagt tggtgaaatc gatggtaccg ttccaatagt tgtgtcgtcc
     9001 gagacttcta gcccaggtgg tctttccggt acgagttggt ccgcagatgt agaggctggg
     9061 gtgtcggatt ccattccttc cattgtcctt gttaaatcgg ccatccattc aaggtcagat
     9121 tgagcttgtt ggtatgagac aggatgtatg taagtataag cgtctatgct tacatggtat
     9181 agatgggttt ccctccagga gtgtagatct tcgtggcagc gaagatctga ttctgtgaag
     9241 ggcgacacat acggttcagg ttgtggaggg aataatttgt tggctgaata ttccagccat
     9301 tgaagctttg ttgcccattc atgagggaat tcttccttga tcatgtcaag atattcctcc
     9361 ttagacgttg cagtctggat aatagttctc catcgtgcgt cagatttgcg aggagaaacc
     9421 ttatgatctc ggaaatctcc tctggtttta atatctccgt cctttgatat gtaatcaagg
     9481 acttgtttag agtttctagc tggctggata ttagggtgat ttccttcaaa atcgaaaaaa
     9541 gaaggatccc taatacaagg ttttttatca agctggagaa gagcatgata gtgggtagtg
     9601 ccatcttgat gaagctcaga agcaacacca aggaagaaaa taagaaaagg tgtgagtttc
     9661 tcccagagaa actggaataa atcatctctt tgagatgagc acttgggata ggtaaggaaa
     9721 acatatttag attggagtct gaagttctta ctagcagaag gcatgttgtt gtgactccga
     9781 ggggttgcct caaactctat cttataaccg gcgtggaggc atggaggcag gggtattttg
     9841 gtcattttaa tagatagtgg aaaatgacgt ggaatttact taaagacgaa gtctttgcga
     9901 caaggggggg cccacgccga atttaatatt accggcgtgg ccccccctta tcgcgagtgc
     9961 tttagcacga gcggtccaga tttaaagtag aaaatttccc gcccactagg gttaaaggtg
    10021 ttcacactat aaaagcatat acgatgtgat ggtatttgat ggagcgtata ttgtatcagg
    10081 tatttccgtt ggatacgaat tattcgtacg accctcatag tttaaactat cagtgtttga
    10141 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
    10201 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
    10261 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
    10321 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
    10381 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
    10441 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
    10501 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
    10561 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
    10621 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
    10681 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
    10741 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
    10801 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
    10861 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg
    10921 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
    10981 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
    11041 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
    11101 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
    11161 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
    11221 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acggctcaac cgtgcggctg
    11281 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc
    11341 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
    11401 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
    11461 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
    11521 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
    11581 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
    11641 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
    11701 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
    11761 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
    11821 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
    11881 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
    11941 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
    12001 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
    12061 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
    12121 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
    12181 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
    12241 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
    12301 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
    12361 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
    12421 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc
    12481 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc
    12541 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc
    12601 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
    12661 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat
    12721 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt
    12781 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga
    12841 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc
    12901 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca
    12961 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt
    13021 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa
    13081 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac
    13141 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg
    13201 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga
    13261 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga
    13321 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc
    13381 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg
    13441 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga
    13501 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
    13561 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat
    13621 tgccctagca ggggaaaaag gtcgaaaagg cctctttcct gtggatagca cgtacattgg
    13681 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat
    13741 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc
    13801 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc
    13861 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct
    13921 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct
    13981 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg
    14041 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    14101 tgcagctccc ggaaacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    14161 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
    14221 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
    14281 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    14341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    14401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    14461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    14521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    14581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    14641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    14701 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    14761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    14821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    14881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    14941 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    15001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    15061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    15121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    15181 ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    15241 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    15301 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    15361 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    15421 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    15481 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    15541 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    15601 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    15661 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    15721 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    15781 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    15841 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    15901 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    15961 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    16021 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    16081 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    16141 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc
    16201 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
    16261 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
    16321 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
    16381 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
    16441 acaaattgac gcttagacaa cttaataaca cattgcggac gtwataacac attgcggacg
    16501 tttttaatgt ag
//
