LOCUS       sequence-182718-       10642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector expressing TALENs targeting Medtr4g020620 and 
            Medtr2g013650A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10642)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 10642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pSC1
FEATURES             Location/Qualifiers
     source          1..10642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(100..915)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(100..915)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     823..842
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     907..926
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1206..1641
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    1788..1812
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1835..2087)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2107..2658)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(2700..2879)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3458..3803
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3746..3766
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3925..3990
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3997..4017
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6739..6795
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6799..6864
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6871..6891
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9010..9597
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      9611..9859
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    9866..9890
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9983..10571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10063..10082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
       61 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      121 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      181 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      241 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      301 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      361 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      421 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gccgaaatac
      481 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      541 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      601 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      661 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      721 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      781 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      841 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
      901 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      961 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1021 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1081 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1141 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1201 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1261 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     1321 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     1381 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     1441 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     1501 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     1561 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     1621 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     1681 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     1741 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcctgg caggatatat
     1801 tgtggtgtaa acgtgtacgc gttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
     1861 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     1921 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     1981 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     2041 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
     2101 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
     2161 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
     2221 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
     2281 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
     2341 gagcgtggag cccagtcccg tccgctggtg gcgggggctg acgtacacgg tggactcggc
     2401 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
     2461 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
     2521 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
     2581 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
     2641 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
     2701 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     2761 gctagatgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     2821 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     2881 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     2941 ccttaattct ccgctcatga tcggcgcgcc agatttgcct tttcaatttc agaaagaatg
     3001 ctaacccaca gatggttaga gaggcttacg cagcaggtat catcaagacg atctacccga
     3061 gcaataatct ccaggaaatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     3121 tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta
     3181 ttccagtatg gacgattcaa ggcttgcttc acaaaccaag gcaagtaata gagattggag
     3241 tctctaaaaa ggtagttccc actgaatcaa aggccatgga gtcaaagatt caaatagagg
     3301 acctaacaga actcgccgta aagactggcg aacagttcat acagagtctc ttacgactca
     3361 atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cacacttgtc tactccaaaa
     3421 atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa
     3481 tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag
     3541 tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg
     3601 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     3661 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     3721 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     3781 gttcatttca tttggagaga acacggggga ctcctgcagg gaattccaag caacgaactg
     3841 cgagtgattc aagaaaaaag aaaacctgag ctttcgatct cttcggagtg gtttcttgtt
     3901 ctttgaaaaa gagggggaag catggattat aaggatcacg atggcgacta caaggaccac
     3961 gatattgact acaaagacga tgacgataaa atggctccta agaaaaagcg caaagtcggt
     4021 atccatggcg ttccctctag aatggtggat ctacgcacgc tcggctacag tcagcagcag
     4081 caagagaaga tcaaaccgaa ggtgcgttcg acagtggcgc agcaccacga ggcactggtg
     4141 ggccatgggt ttacacacgc gcacatcgtt gcgctcagcc aacacccggc agcgttaggg
     4201 accgtcgctg tcacgtatca gcacataatc acggcgttgc cagaggcgac acacgaagac
     4261 atcgttggcg tcggcaaaca gtggtccggc gcacgcgccc tggaggcctt gctcacggat
     4321 gcgggggagt tgagaggtcc gccgttacag ttggacacag gccaacttgt gaagattgca
     4381 aaacgtggcg gcgtgaccgc aatggaggca gtgcatgcca gcaggaatgc gttaacaggt
     4441 gcccccctga acctgacccc ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag
     4501 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
     4561 ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg
     4621 cagcggctgt tgccggtgct gtgccaggac catggcctga ctccggacca agtggtggct
     4681 atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
     4741 ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacattggc
     4801 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
     4861 ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa
     4921 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
     4981 gtggctatcg ccagcaacat tggcggcaag caagcgctcg aaacggtgca gcggctgttg
     5041 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
     5101 attggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
     5161 catggcctga ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg
     5221 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac
     5281 caagtggtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg
     5341 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
     5401 agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
     5461 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagccacga tggcggcaag
     5521 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
     5581 ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg
     5641 cagcggctgt tgccggtgct gtgccaggac catggcctga ctccggacca agtggtggct
     5701 atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
     5761 ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacattggc
     5821 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
     5881 ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa
     5941 agcattgtgg cccagctgag ccggcctgat ccggcgttgg ccgcgttgac caacgaccgc
     6001 ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa aaagggattg
     6061 ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg cacgtcccat
     6121 cgcgttgccg gatcccagct ggtgaagtcc gagctggaag aaaaaaagag cgagctgcgc
     6181 cacaagctca agtacgtgcc ccacgagtac atcgagctga tcgagatcgc ccgcaacagc
     6241 acccaagacc gcatcctgga gatgaaagtg atggagttct tcatgaaggt gtacggctac
     6301 cgcggcaagc acctgggcgg ctcccgcaag cccgatggcg ccatctacac cgtgggctcc
     6361 cccatcgact atggcgtcat tgtcgacacc aaggcctact ccggcggcta caacttaccc
     6421 atcggtcagg ccgacgagat gcaacgctac gtgaaggaga accagacccg caataagcac
     6481 attaatccca acgagtggtg gaaggtgtac ccctcctccg tgaccgagtt caaattcctg
     6541 ttcgtgtccg gccacttcaa gggcaattat aaggcccaac tgacccgcct gaaccgcaag
     6601 accaactgca acggcgccgt gctgtccgtg gaggaactgc tgatcggcgg cgagatgatc
     6661 aaggctggta ccctgaccct ggaagaggtg cgccgcaagt tcaacaatgg tgaaatcaat
     6721 ttcaggtccg gaagcggcgc cacgaacttc tctctgttaa agcaagcagg tgacgtggaa
     6781 gaaaacccag ggcccatgga ctacaaagac catgacggtg attataaaga tcatgacatc
     6841 gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt gggcattcac
     6901 ggggtgccgg ctagcatggt ggatctacgc acgctcggct acagtcagca gcagcaagag
     6961 aagatcaaac cgaaggtgcg ttcgacagtg gcgcagcacc acgaggcact ggtgggccat
     7021 gggtttacac acgcgcacat cgttgcgctc agccaacacc cggcagcgtt agggaccgtc
     7081 gctgtcacgt atcagcacat aatcacggcg ttgccagagg cgacacacga agacatcgtt
     7141 ggcgtcggca aacagtggtc cggcgcacgc gccctggagg ccttgctcac ggatgcgggg
     7201 gagttgagag gtccgccgtt acagttggac acaggccaac ttgtgaagat tgcaaaacgt
     7261 ggcggcgtga ccgcaatgga ggcagtgcat gcatcgcgca atgcactgac gggtgccccc
     7321 ctgaacctga ccccggacca agtggtggct atcgccagcc acgatggcgg caagcaagcg
     7381 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac
     7441 caagtggtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg
     7501 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
     7561 agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
     7621 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag
     7681 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgact
     7741 ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg
     7801 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
     7861 atcgccagca acattggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
     7921 ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc
     7981 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
     8041 ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa
     8101 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
     8161 gtggctatcg ccagcaacat tggcggcaag caagcgctcg aaacggtgca gcggctgttg
     8221 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
     8281 attggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
     8341 catggcctga ccccggacca agtggtggct atcgccagcc acgatggcgg caagcaagcg
     8401 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
     8461 caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg
     8521 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
     8581 agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
     8641 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag
     8701 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
     8761 ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaagcatt
     8821 gtggcccagc tgagccggcc tgatccggcg ttggccgcgt tgaccaacga tcatctggtg
     8881 gcactcgcgt gcctcggcgg acgtcctgcc atggatgcag tgaaaaaggg attgccgcac
     8941 gcgccggaat tgatcagaag agtcaatcgc cgtattggcg aacgcacgtc ccatcgcgtt
     9001 gccagatctc aactagtcaa aagtgaactg gaggagaaga aatctgaact tcgtcataaa
     9061 ttgaaatatg tgcctcatga atatattgaa ttaattgaaa ttgccagaaa ttccactcag
     9121 gatagaattc ttgaaatgaa ggtaatggaa ttttttatga aagtttatgg atatagaggt
     9181 aaacatttgg gtggatcaag gaaaccggac ggagcaattt atactgtcgg atctcctatt
     9241 gattacggtg tgatcgtgga tactaaagct tatagcggag gttataatct gccaattggc
     9301 caagcagatg aaatggagcg atatgtcgaa gaaaatcaaa cacgagacaa acatctcaac
     9361 cctaatgaat ggtggaaagt ctatccatct tctgtaacgg aatttaagtt tttatttgtg
     9421 agtggtcact ttaaaggaaa ctacaaagct cagcttacac gattaaatca tatcactaat
     9481 tgtaatggag ctgttcttag tgtagaagag cttttaattg gtggagaaat gattaaagcc
     9541 ggcacattaa ccttagagga agtgaggcgg aaatttaata acggcgagat aaacttttaa
     9601 tagctcgaga tatgaagatg aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg
     9661 cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt tttctaatat
     9721 taaatgaatg taagatcaca ttataatgaa taaacaaatg tttctataat ccattgtgaa
     9781 tgttttgttg gatctcttct gcagcatata actactgtat gtgctatggt atggactatg
     9841 gaatatgatt aaagataagg agctctgaca ggatatattg gcgggtaaac taagtcgctg
     9901 tatgtgtttg tttgatttaa atcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9961 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    10021 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    10081 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    10141 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    10201 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    10261 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    10321 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    10381 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    10441 gcgctctgct gaagccagtt accttcggaa gaagagttgg tagctcttga tccggcaaac
    10501 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    10561 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    10621 actcacgtta agggattttg gt
//
