LOCUS       sequence-182758-       10048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast vector for targeted deletion of 6 genes in tomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 10048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC362
FEATURES             Location/Qualifiers
     source          1..10048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     826..843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1389..1734
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1677..1697
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2326..2382
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2383..6486
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2383..6486
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6499..6519
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6530..6778
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     protein_bind    7275..7294
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        7315..7390
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7391..7410
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        7431..7506
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7507..7526
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        7547..7622
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7623..7642
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        7663..7738
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7739..7758
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        7779..7854
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7855..7874
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        7895..7970
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7971..7990
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        8011..8086
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8087..8106
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        8127..8202
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8203..8222
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        8243..8318
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8319..8338
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        8359..8434
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8435..8454
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        8475..8550
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8551..8570
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        8591..8666
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8667..8686
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     polyA_signal    8706..8880
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             9248..10039
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg gagagcgccc atatgcgcac tcctcgcatg cggcgcgccg atcggcgcgc
      901 cagatttgcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      961 gcagcaggta tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
     1021 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
     1081 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1141 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1201 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1261 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1321 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1381 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1441 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1501 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1561 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1621 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1681 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1741 actcctgcag gaaaaatgga tcattatctt gatattagac ttagacctga tccagaattt
     1801 ccaccagctc aacttatgtc tgttcttttt ggaaaacttc atcaagctct tgttgctcaa
     1861 ggaggagata gaattggagt ttcttttcct gatcttgatg aatcaagatc aagacttgga
     1921 gaaagactta gaattcatgc ttctgctgat gatcttagag ctttgcttgc tagaccttgg
     1981 cttgaaggac ttagagatca tcttcaattt ggagaaccag ctgttgttcc acatccaact
     2041 ccttatagac aagtttcaag agttcaagct aaatctaatc cagaaagact tagaagaaga
     2101 cttatgagaa gacatgatct ttctgaagaa gaagctagaa aaagaattcc tgatactgtt
     2161 gctagagctt tggatttgcc ttttgttaca cttagatcac aatctactgg acaacatttt
     2221 agacttttta ttagacatgg accacttcaa gttactgctg aagaaggagg atttacttgt
     2281 tatggacttt ctaagggagg ttttgttcct tggtttggat ctggagctac taatttttct
     2341 cttcttaagc aagctggaga tgttgaagaa aatcctggac ccatggataa gaagtactct
     2401 atcggactcg atatcggaac taactctgtg ggatgggctg tgatcaccga tgagtacaag
     2461 gtgccatcta agaagttcaa ggttctcgga aacaccgata ggcactctat caagaaaaac
     2521 cttatcggtg ctctcctctt cgattctggt gaaactgctg aggctaccag actcaagaga
     2581 accgctagaa gaaggtacac cagaagaaag aacaggatct gctacctcca agagatcttc
     2641 tctaacgaga tggctaaagt ggatgattca ttcttccaca ggctcgaaga gtcattcctc
     2701 gtggaagaag ataagaagca cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg
     2761 gcataccacg agaagtaccc tactatctac cacctcagaa agaagctcgt tgattctact
     2821 gataaggctg atctcaggct catctacctc gctctcgctc acatgatcaa gttcagagga
     2881 cacttcctca tcgagggtga tctcaaccct gataactctg atgtggataa gttgttcatc
     2941 cagctcgtgc agacctacaa ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg
     3001 gatgctaagg ctatcctctc tgctaggctc tctaagtcaa gaaggcttga gaacctcatt
     3061 gctcagctcc ctggtgagaa gaagaacgga cttttcggaa acttgatcgc tctctctctc
     3121 ggactcaccc ctaacttcaa gtctaacttc gatctcgctg aggatgcaaa gctccagctc
     3181 tcaaaggata cctacgatga tgatctcgat aacctcctcg ctcagatcgg agatcagtac
     3241 gctgatttgt tcctcgctgc taagaacctc tctgatgcta tcctcctcag tgatatcctc
     3301 agagtgaaca ccgagatcac caaggctcca ctctcagctt ctatgatcaa gagatacgat
     3361 gagcaccacc aggatctcac acttctcaag gctcttgtta gacagcagct cccagagaag
     3421 tacaaagaga ttttcttcga tcagtctaag aacggatacg ctggttacat cgatggtggt
     3481 gcatctcaag aagagttcta caagttcatc aagcctatcc tcgagaagat ggatggaacc
     3541 gaggaactcc tcgtgaagct caatagagag gatcttctca gaaagcagag gaccttcgat
     3601 aacggatcta tccctcatca gatccacctc ggagagttgc acgctatcct tagaaggcaa
     3661 gaggatttct acccattcct caaggataac agggaaaaga ttgagaagat tctcaccttc
     3721 agaatccctt actacgtggg acctctcgct agaggaaact caagattcgc ttggatgacc
     3781 agaaagtctg aggaaaccat caccccttgg aacttcgaag aggtggtgga taagggtgct
     3841 agtgctcagt ctttcatcga gaggatgacc aacttcgata agaaccttcc aaacgagaag
     3901 gtgctcccta agcactcttt gctctacgag tacttcaccg tgtacaacga gttgaccaag
     3961 gttaagtacg tgaccgaggg aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag
     4021 gctatcgttg atctcttgtt caagaccaac agaaaggtga ccgtgaagca gctcaaagag
     4081 gattacttca agaaaatcga gtgcttcgat tcagttgaga tttctggtgt tgaggatagg
     4141 ttcaacgcat ctctcggaac ctaccacgat ctcctcaaga tcattaagga taaggatttc
     4201 ttggataacg aggaaaacga ggatatcttg gaggatatcg ttcttaccct caccctcttt
     4261 gaagatagag agatgattga agaaaggctc aagacctacg ctcatctctt cgatgataag
     4321 gtgatgaagc agttgaagag aagaagatac actggttggg gaaggctctc aagaaagctc
     4381 attaacggaa tcagggataa gcagtctgga aagacaatcc ttgatttcct caagtctgat
     4441 ggattcgcta acagaaactt catgcagctc atccacgatg attctctcac ctttaaagag
     4501 gatatccaga aggctcaggt ttcaggacag ggtgatagtc tccatgagca tatcgctaac
     4561 ctcgctggat ctcctgcaat caagaaggga atcctccaga ctgtgaaggt tgtggatgag
     4621 ttggtgaagg tgatgggaag gcataagcct gagaacatcg tgatcgaaat ggctagagag
     4681 aaccagacca ctcagaaggg acagaagaac tctagggaaa ggatgaagag gatcgaggaa
     4741 ggtatcaaag agcttggatc tcagatcctc aaagagcacc ctgttgagaa cactcagctc
     4801 cagaatgaga agctctacct ctactacctc cagaacggaa gggatatgta tgtggatcaa
     4861 gagttggata tcaacaggct ctctgattac gatgttgatc atatcgtgcc acagtcattc
     4921 ttgaaggatg attctatcga taacaaggtg ctcaccaggt ctgataagaa caggggtaag
     4981 agtgataacg tgccaagtga agaggttgtg aagaaaatga agaactattg gaggcagctc
     5041 ctcaacgcta agctcatcac tcagagaaag ttcgataact tgactaaggc tgagagggga
     5101 ggactctctg aattggataa ggcaggattc atcaagaggc agcttgtgga aaccaggcag
     5161 atcactaagc acgttgcaca gatcctcgat tctaggatga acaccaagta cgatgagaac
     5221 gataagttga tcagggaagt gaaggttatc accctcaagt caaagctcgt gtctgatttc
     5281 agaaaggatt tccaattcta caaggtgagg gaaatcaaca actaccacca cgctcacgat
     5341 gcttacctta acgctgttgt tggaaccgct ctcatcaaga agtatcctaa gctcgagtca
     5401 gagttcgtgt acggtgatta caaggtgtac gatgtgagga agatgatcgc taagtctgag
     5461 caagagatcg gaaaggctac cgctaagtat ttcttctact ctaacatcat gaatttcttc
     5521 aagaccgaga ttaccctcgc taacggtgag atcagaaaga ggccactcat cgagacaaac
     5581 ggtgaaacag gtgagatcgt gtgggataag ggaagggatt tcgctaccgt tagaaaggtg
     5641 ctctctatgc cacaggtgaa catcgttaag aaaaccgagg tgcagaccgg tggattctct
     5701 aaagagtcta tcctccctaa gaggaactct gataagctca ttgctaggaa gaaggattgg
     5761 gaccctaaga aatacggtgg tttcgattct cctaccgtgg cttactctgt tctcgttgtg
     5821 gctaaggttg agaagggaaa gagtaagaag ctcaagtctg ttaaggaact tctcggaatc
     5881 actatcatgg aaaggtcatc tttcgagaag aacccaatcg atttcctcga ggctaaggga
     5941 tacaaagagg ttaagaagga tctcatcatc aagctcccaa agtactcact cttcgaactc
     6001 gagaacggta gaaagaggat gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt
     6061 gctctcccat ctaagtacgt taactttctt tacctcgctt ctcactacga gaagttgaag
     6121 ggatctccag aagataacga gcagaagcaa cttttcgttg agcagcacaa gcactacttg
     6181 gatgagatca tcgagcagat ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac
     6241 ctcgataagg tgttgtctgc ttacaacaag cacagagata agcctatcag ggaacaggca
     6301 gagaacatca tccatctctt cacccttacc aacctcggtg ctcctgctgc tttcaagtac
     6361 ttcgatacaa ccatcgatag gaagagatac acctctacca aagaagtgct cgatgctacc
     6421 ctcatccatc agtctatcac tggactctac gagactagga tcgatctctc acagctcggt
     6481 ggtgattcaa gggctgatcc taagaagaag aggaaggttt gactcgagat atgaagatga
     6541 agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct
     6601 tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aagatcacat
     6661 tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctg
     6721 cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta aagataagga
     6781 gctccggtga cggacggcgc gctggcagac atactgtccc acaaatgaag atggaatctg
     6841 taaaagaaaa cgcgtgaaat aatgcgtctg acaaaggtta ggtcggctgc ctttaatcaa
     6901 taccaaagtg gtccctacca cgatggaaaa actgtgcagt cggtttggct ttttctgacg
     6961 aacaaataag attcgtggcc gacaggtggg ggtccaccat gtgaaggcat cttcagactc
     7021 caataatgga gcaatgacgt aagggcttac gaaataagta agggtagttt gggaaatgtc
     7081 cactcacccg tcagtctata aatacttagc ccctccctca ttgttaaggg agcaaaatct
     7141 cagagagata gtcctagaga gagaaagaga gcaagtagcc tagaagtagt caaggcggcg
     7201 aagtattcag gcacgtggcc aggaagaaga aaagccaaga cgacgaaaac aggtaagagc
     7261 taagcttcct gcaggttcac tgccgtatag gcagatgata gatccagatg ttccgtttta
     7321 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
     7381 gagtcggtgc gttcactgcc gtataggcag aggattgtca cagacattcc gttttagagc
     7441 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     7501 cggtgcgttc actgccgtat aggcagttct tcttcttcag acacgagttt tagagctaga
     7561 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     7621 gcgttcactg ccgtataggc aggatctctt gaaatcttcg tagttttaga gctagaaata
     7681 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgcgt
     7741 tcactgccgt ataggcaggg ggcagataca tagtgagggt tttagagcta gaaatagcaa
     7801 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcgttcac
     7861 tgccgtatag gcagggtgca ttatattgtt tgctgtttta gagctagaaa tagcaagtta
     7921 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc gttcactgcc
     7981 gtataggcag ttatcggagc tggccctgct gttttagagc tagaaatagc aagttaaaat
     8041 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgttc actgccgtat
     8101 aggcagggat agttcatcca tcaacagttt tagagctaga aatagcaagt taaaataagg
     8161 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcgttcactg ccgtataggc
     8221 agcatattct tgaagtttag ctgttttaga gctagaaata gcaagttaaa ataaggctag
     8281 tccgttatca acttgaaaaa gtggcaccga gtcggtgcgt tcactgccgt ataggcagag
     8341 tccagaagaa gaagaatggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     8401 ttatcaactt gaaaaagtgg caccgagtcg gtgcgttcac tgccgtatag gcaggagtac
     8461 ttacgacgaa tcgagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     8521 caacttgaaa aagtggcacc gagtcggtgc gttcactgcc gtataggcag atgaatcggt
     8581 tgtacgcaga gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     8641 ttgaaaaagt ggcaccgagt cggtgcgttc actgccgtat aggcaggtcg atcgacaagc
     8701 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
     8761 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
     8821 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
     8881 ccccgaatta agtgccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     8941 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     9001 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     9061 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     9121 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     9181 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     9241 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     9301 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     9361 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     9421 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     9481 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     9541 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     9601 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     9661 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     9721 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     9781 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     9841 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     9901 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     9961 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
    10021 caaggtagtc ggcaaataac cctcgagc
//
