LOCUS       sequence-182763-        8757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast vector expressing gRNA23 targeting tomato ANT1 (with 
            TREX2 expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC391
FEATURES             Location/Qualifiers
     source          1..8757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     826..843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1389..1734
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1677..1697
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2473..2529
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2530..6633
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2530..6633
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6646..6666
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6677..6925
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        7414..7489
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             7957..8748
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg gagagcgccc atatgcgcac tcctcgcatg cggcgcgccg atcggcgcgc
      901 cagatttgcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      961 gcagcaggta tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
     1021 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
     1081 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1141 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1201 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1261 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1321 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1381 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1441 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1501 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1561 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1621 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1681 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1741 actcctgcag gaaaaatgtc tgagccacct cgggctgaga cctttgtatt cctggaccta
     1801 gaagccactg ggctcccaaa catggaccct gagattgcag agatatccct ttttgctgtt
     1861 caccgctctt ccctggagaa cccagaacgg gatgattctg gttccttggt gctgccccgt
     1921 gttctggaca agctcacact gtgcatgtgc ccggagcgcc cctttactgc caaggccagt
     1981 gagattactg gtttgagcag cgaaagcctg atgcactgcg ggaaggctgg tttcaatggc
     2041 gctgtggtaa ggacactgca gggcttccta agccgccagg agggccccat ctgccttgtg
     2101 gcccacaatg gcttcgatta tgacttccca ctgctgtgca cggagctaca acgtctgggt
     2161 gcccatctgc cccaagacac tgtctgcctg gacacactgc ctgcattgcg gggcctggac
     2221 cgtgctcaca gccacggcac cagggctcaa ggccgcaaaa gctacagcct ggccagtctc
     2281 ttccaccgct acttccaggc tgaacccagt gctgcccatt cagcagaagg tgatgtgcac
     2341 accctgcttc tgatcttcct gcatcgtgct cctgagctgc tcgcctgggc agatgagcag
     2401 gcccgcagct gggctcatat tgagcccatg tacgtgccac ctgatggtcc aagcctcgaa
     2461 gccggatctg gagctactaa tttttctctt cttaagcaag ctggagatgt tgaagaaaat
     2521 cctggaccca tggataagaa gtactctatc ggactcgata tcggaactaa ctctgtggga
     2581 tgggctgtga tcaccgatga gtacaaggtg ccatctaaga agttcaaggt tctcggaaac
     2641 accgataggc actctatcaa gaaaaacctt atcggtgctc tcctcttcga ttctggtgaa
     2701 actgctgagg ctaccagact caagagaacc gctagaagaa ggtacaccag aagaaagaac
     2761 aggatctgct acctccaaga gatcttctct aacgagatgg ctaaagtgga tgattcattc
     2821 ttccacaggc tcgaagagtc attcctcgtg gaagaagata agaagcacga gaggcaccct
     2881 atcttcggaa acatcgttga tgaggtggca taccacgaga agtaccctac tatctaccac
     2941 ctcagaaaga agctcgttga ttctactgat aaggctgatc tcaggctcat ctacctcgct
     3001 ctcgctcaca tgatcaagtt cagaggacac ttcctcatcg agggtgatct caaccctgat
     3061 aactctgatg tggataagtt gttcatccag ctcgtgcaga cctacaacca gcttttcgaa
     3121 gagaacccta tcaacgcttc aggtgtggat gctaaggcta tcctctctgc taggctctct
     3181 aagtcaagaa ggcttgagaa cctcattgct cagctccctg gtgagaagaa gaacggactt
     3241 ttcggaaact tgatcgctct ctctctcgga ctcaccccta acttcaagtc taacttcgat
     3301 ctcgctgagg atgcaaagct ccagctctca aaggatacct acgatgatga tctcgataac
     3361 ctcctcgctc agatcggaga tcagtacgct gatttgttcc tcgctgctaa gaacctctct
     3421 gatgctatcc tcctcagtga tatcctcaga gtgaacaccg agatcaccaa ggctccactc
     3481 tcagcttcta tgatcaagag atacgatgag caccaccagg atctcacact tctcaaggct
     3541 cttgttagac agcagctccc agagaagtac aaagagattt tcttcgatca gtctaagaac
     3601 ggatacgctg gttacatcga tggtggtgca tctcaagaag agttctacaa gttcatcaag
     3661 cctatcctcg agaagatgga tggaaccgag gaactcctcg tgaagctcaa tagagaggat
     3721 cttctcagaa agcagaggac cttcgataac ggatctatcc ctcatcagat ccacctcgga
     3781 gagttgcacg ctatccttag aaggcaagag gatttctacc cattcctcaa ggataacagg
     3841 gaaaagattg agaagattct caccttcaga atcccttact acgtgggacc tctcgctaga
     3901 ggaaactcaa gattcgcttg gatgaccaga aagtctgagg aaaccatcac cccttggaac
     3961 ttcgaagagg tggtggataa gggtgctagt gctcagtctt tcatcgagag gatgaccaac
     4021 ttcgataaga accttccaaa cgagaaggtg ctccctaagc actctttgct ctacgagtac
     4081 ttcaccgtgt acaacgagtt gaccaaggtt aagtacgtga ccgagggaat gaggaagcct
     4141 gcttttttgt caggtgagca aaagaaggct atcgttgatc tcttgttcaa gaccaacaga
     4201 aaggtgaccg tgaagcagct caaagaggat tacttcaaga aaatcgagtg cttcgattca
     4261 gttgagattt ctggtgttga ggataggttc aacgcatctc tcggaaccta ccacgatctc
     4321 ctcaagatca ttaaggataa ggatttcttg gataacgagg aaaacgagga tatcttggag
     4381 gatatcgttc ttaccctcac cctctttgaa gatagagaga tgattgaaga aaggctcaag
     4441 acctacgctc atctcttcga tgataaggtg atgaagcagt tgaagagaag aagatacact
     4501 ggttggggaa ggctctcaag aaagctcatt aacggaatca gggataagca gtctggaaag
     4561 acaatccttg atttcctcaa gtctgatgga ttcgctaaca gaaacttcat gcagctcatc
     4621 cacgatgatt ctctcacctt taaagaggat atccagaagg ctcaggtttc aggacagggt
     4681 gatagtctcc atgagcatat cgctaacctc gctggatctc ctgcaatcaa gaagggaatc
     4741 ctccagactg tgaaggttgt ggatgagttg gtgaaggtga tgggaaggca taagcctgag
     4801 aacatcgtga tcgaaatggc tagagagaac cagaccactc agaagggaca gaagaactct
     4861 agggaaagga tgaagaggat cgaggaaggt atcaaagagc ttggatctca gatcctcaaa
     4921 gagcaccctg ttgagaacac tcagctccag aatgagaagc tctacctcta ctacctccag
     4981 aacggaaggg atatgtatgt ggatcaagag ttggatatca acaggctctc tgattacgat
     5041 gttgatcata tcgtgccaca gtcattcttg aaggatgatt ctatcgataa caaggtgctc
     5101 accaggtctg ataagaacag gggtaagagt gataacgtgc caagtgaaga ggttgtgaag
     5161 aaaatgaaga actattggag gcagctcctc aacgctaagc tcatcactca gagaaagttc
     5221 gataacttga ctaaggctga gaggggagga ctctctgaat tggataaggc aggattcatc
     5281 aagaggcagc ttgtggaaac caggcagatc actaagcacg ttgcacagat cctcgattct
     5341 aggatgaaca ccaagtacga tgagaacgat aagttgatca gggaagtgaa ggttatcacc
     5401 ctcaagtcaa agctcgtgtc tgatttcaga aaggatttcc aattctacaa ggtgagggaa
     5461 atcaacaact accaccacgc tcacgatgct taccttaacg ctgttgttgg aaccgctctc
     5521 atcaagaagt atcctaagct cgagtcagag ttcgtgtacg gtgattacaa ggtgtacgat
     5581 gtgaggaaga tgatcgctaa gtctgagcaa gagatcggaa aggctaccgc taagtatttc
     5641 ttctactcta acatcatgaa tttcttcaag accgagatta ccctcgctaa cggtgagatc
     5701 agaaagaggc cactcatcga gacaaacggt gaaacaggtg agatcgtgtg ggataaggga
     5761 agggatttcg ctaccgttag aaaggtgctc tctatgccac aggtgaacat cgttaagaaa
     5821 accgaggtgc agaccggtgg attctctaaa gagtctatcc tccctaagag gaactctgat
     5881 aagctcattg ctaggaagaa ggattgggac cctaagaaat acggtggttt cgattctcct
     5941 accgtggctt actctgttct cgttgtggct aaggttgaga agggaaagag taagaagctc
     6001 aagtctgtta aggaacttct cggaatcact atcatggaaa ggtcatcttt cgagaagaac
     6061 ccaatcgatt tcctcgaggc taagggatac aaagaggtta agaaggatct catcatcaag
     6121 ctcccaaagt actcactctt cgaactcgag aacggtagaa agaggatgct cgcttctgct
     6181 ggtgagcttc aaaagggaaa cgagcttgct ctcccatcta agtacgttaa ctttctttac
     6241 ctcgcttctc actacgagaa gttgaaggga tctccagaag ataacgagca gaagcaactt
     6301 ttcgttgagc agcacaagca ctacttggat gagatcatcg agcagatctc tgagttctct
     6361 aaaagggtga tcctcgctga tgcaaacctc gataaggtgt tgtctgctta caacaagcac
     6421 agagataagc ctatcaggga acaggcagag aacatcatcc atctcttcac ccttaccaac
     6481 ctcggtgctc ctgctgcttt caagtacttc gatacaacca tcgataggaa gagatacacc
     6541 tctaccaaag aagtgctcga tgctaccctc atccatcagt ctatcactgg actctacgag
     6601 actaggatcg atctctcaca gctcggtggt gattcaaggg ctgatcctaa gaagaagagg
     6661 aaggtttgac tcgagatatg aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct
     6721 tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt tatgaatttg tggctttttc
     6781 taatattaaa tgaatgtaag atcacattat aatgaataaa caaatgtttc tataatccat
     6841 tgtgaatgtt ttgttggatc tcttctgcag catataacta ctgtatgtgc tatggtatgg
     6901 actatggaat atgattaaag ataaggagct ccggtgacgg acccatggct tcgttgaaca
     6961 acggaaactc gacttgcctt ccgcacaata catcatttct tcttagcttt ttttcttctt
     7021 cttcgttcat acagtttttt tttgtttatc agcttacatt ttcttgaacc gtagctttcg
     7081 ttttcttctt tttaactttc cattcggagt ttttgtatct tgtttcatag tttgtcccag
     7141 gattagaatg attaggcatc gaaccttcaa gaatttgatt gaataaaaca tcttcattct
     7201 taagatatga agataatctt caaaaggccc ctgggaatct gaaagaagag aagcaggccc
     7261 atttatatgg gaaagaacaa tagtatttct tatataggcc catttaagtt gaaaacaatc
     7321 ttcaaaagtc ccacatcgct tagataagaa aacgaagctg agtttatata cagctagagt
     7381 cgaagtagtg attgttacgt tattttacgt agagttttag agctagaaat agcaagttaa
     7441 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttcccg
     7501 gcgagctttg ggtacgtcac gtggctcgag cgcgtagtcc tcggtaatat cgcagaacaa
     7561 aagtacctga tatcgagtgt acttcaagtc acaccggcga gtgccagcca ggacagaaat
     7621 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     7681 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     7741 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     7801 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     7861 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     7921 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
     7981 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     8041 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     8101 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     8161 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     8221 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     8281 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     8341 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     8401 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     8461 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     8521 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     8581 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     8641 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     8701 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagc
//
