LOCUS       sequence-182764-        9068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast vector expressing gRNA38 targeting barley MLO (control 
            without TREX2 expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC440
FEATURES             Location/Qualifiers
     source          1..9068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     826..843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             2916..7019
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2916..7019
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7020..7067
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      7078..7326
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        7726..7801
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             8268..9059
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg gagagcgccc atatgcgcac tcctcgcatg cggcgcgccg atctgcaggc
      901 gcgcctgcag tgcagcgtga cccggtcgtg cccctctcta gagataatga gcattgcatg
      961 tctaagttat aaaaaattac cacatatttt ttttgtcaca cttgtttgaa gtgcagttta
     1021 tctatcttta tacatatatt taaactttac tctacgaata atataatcta tagtactaca
     1081 ataatatcag tgttttagag aatcatataa atgaacagtt agacatggtc taaaggacaa
     1141 ttgagtattt tgacaacagg actctacagt tttatctttt tagtgtgcat gtgttctcct
     1201 ttttttttgc aaatagcttc acctatataa tacttcatcc attttattag tacatccatt
     1261 tagggtttag ggttaatggt ttttatagac taattttttt agtacatcta ttttattcta
     1321 ttttagcctc taaattaaga aaactaaaac tctattttag tttttttatt taataattta
     1381 gatataaaat agaataaaat aaagtgacta aaaattaaac aaataccctt taagaaatta
     1441 aaaaaactaa ggaaacattt ttcttgtttc gagtagataa tgccagcctg ttaaacgccg
     1501 tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag
     1561 cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg
     1621 ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg
     1681 gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc
     1741 caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc
     1801 ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa
     1861 tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc ccccctctct
     1921 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1981 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     2041 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     2101 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     2161 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     2221 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     2281 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     2341 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     2401 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     2461 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     2521 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     2581 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     2641 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     2701 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     2761 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     2821 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     2881 gtttggtgtt acttcctgca gggcgatcta ttcgaatgga caagaagtac tcgatcggcc
     2941 tcgacatcgg gacgaactca gttggctggg ccgtgatcac cgacgagtac aaggtgccct
     3001 ctaagaagtt caaggtcctg gggaacaccg accgccattc catcaagaag aacctcatcg
     3061 gcgctctcct gttcgacagc ggggagaccg ctgaggctac gaggctcaag agaaccgcta
     3121 ggcgccggta cacgagaagg aagaacagga tctgctacct ccaagagatt ttctccaacg
     3181 agatggccaa ggttgacgat tcattcttcc accgcctgga ggagtctttc ctcgtggagg
     3241 aggataagaa gcacgagcgg catcccatct tcggcaacat cgtggacgag gttgcctacc
     3301 acgagaagta ccctacgatc taccatctgc ggaagaagct cgtggactcc accgataagg
     3361 cggacctcag actgatctac ctcgctctgg cccacatgat caagttccgc ggccatttcc
     3421 tgatcgaggg ggatctcaac ccagacaaca gcgatgttga caagctgttc atccaactcg
     3481 tgcagaccta caaccaactc ttcgaggaga acccgatcaa cgcctctggc gtggacgcga
     3541 aggctatcct gtccgcgagg ctctcgaagt ccaggaggct ggagaacctg atcgctcagc
     3601 tcccaggcga gaagaagaac ggcctgttcg ggaacctcat cgctctcagc ctggggctca
     3661 ccccgaactt caagtcgaac ttcgatctcg ctgaggacgc caagctgcaa ctctccaagg
     3721 acacctacga cgatgacctc gataacctcc tggcccagat cggcgatcaa tacgcggacc
     3781 tgttcctcgc tgccaagaac ctgtcggacg ccatcctcct gtcagatatc ctccgcgtga
     3841 acaccgagat cacgaaggct ccactctctg cctccatgat caagcgctac gacgagcacc
     3901 atcaggatct gaccctcctg aaggcgctgg tccgccaaca gctcccggag aagtacaagg
     3961 agattttctt cgatcagtcg aagaacggct acgctgggta catcgacggc ggggcctcac
     4021 aagaggagtt ctacaagttc atcaagccaa tcctggagaa gatggacggc acggaggagc
     4081 tcctggtgaa gctcaacagg gaggacctcc tgcggaagca gagaaccttc gataacggca
     4141 gcatccccca ccaaatccat ctcggggagc tgcacgccat cctgagaagg caagaggact
     4201 tctacccttt cctcaaggat aaccgggaga agatcgagaa gatcctgacc ttcagaatcc
     4261 catactacgt cggccctctc gcgcggggga actcaagatt cgcttggatg acccgcaagt
     4321 ctgaggagac catcacgccg tggaacttcg aggaggtggt ggacaagggc gctagcgctc
     4381 agtcgttcat cgagaggatg accaacttcg acaagaacct gcccaacgag aaggtgctcc
     4441 ctaagcactc gctcctgtac gagtacttca ccgtctacaa cgagctcacg aaggtgaagt
     4501 acgtcaccga gggcatgcgc aagccagcgt tcctgtccgg ggagcagaag aaggctatcg
     4561 tggacctcct gttcaagacc aaccggaagg tcacggttaa gcaactcaag gaggactact
     4621 tcaagaagat cgagtgcttc gattcggtcg agatcagcgg cgttgaggac cgcttcaacg
     4681 ccagcctcgg gacctaccac gatctcctga agatcatcaa ggataaggac ttcctggaca
     4741 acgaggagaa cgaggatatc ctggaggaca tcgtgctgac cctcacgctg ttcgaggaca
     4801 gggagatgat cgaggagcgc ctgaagacgt acgcccatct cttcgatgac aaggtcatga
     4861 agcaactcaa gcgccggaga tacaccggct gggggaggct gtcccgcaag ctcatcaacg
     4921 gcatccggga caagcagtcc gggaagacca tcctcgactt cctgaagagc gatggcttcg
     4981 ccaacaggaa cttcatgcaa ctgatccacg atgacagcct caccttcaag gaggatatcc
     5041 aaaaggctca agtgagcggc cagggggact cgctgcacga gcatatcgcg aacctcgctg
     5101 gctcccccgc gatcaagaag ggcatcctcc agaccgtgaa ggttgtggac gagctcgtga
     5161 aggtcatggg ccggcacaag cctgagaaca tcgtcatcga gatggccaga gagaaccaaa
     5221 ccacgcagaa ggggcaaaag aactctaggg agcgcatgaa gcgcatcgag gagggcatca
     5281 aggagctggg gtcccaaatc ctcaaggagc acccagtgga gaacacccaa ctgcagaacg
     5341 agaagctcta cctgtactac ctccagaacg gcagggatat gtacgtggac caagagctgg
     5401 atatcaaccg cctcagcgat tacgacgtcg atcatatcgt tccccagtct ttcctgaagg
     5461 atgactccat cgacaacaag gtcctcacca ggtcggacaa gaaccgcggc aagtcagata
     5521 acgttccatc tgaggaggtc gttaagaaga tgaagaacta ctggaggcag ctcctgaacg
     5581 ccaagctgat cacgcaaagg aagttcgaca acctcaccaa ggctgagaga ggcgggctct
     5641 cagagctgga caaggccggc ttcatcaagc ggcagctggt cgagaccaga caaatcacga
     5701 agcacgttgc gcaaatcctc gactctcgga tgaacacgaa gtacgatgag aacgacaagc
     5761 tgatcaggga ggttaaggtg atcaccctga agtctaagct cgtctccgac ttcaggaagg
     5821 atttccagtt ctacaaggtt cgcgagatca acaactacca ccatgcccat gacgcttacc
     5881 tcaacgctgt ggtcggcacc gctctgatca agaagtaccc aaagctggag tccgagttcg
     5941 tgtacgggga ctacaaggtt tacgatgtgc gcaagatgat cgccaagtcg gagcaagaga
     6001 tcggcaaggc taccgccaag tacttcttct actcaaacat catgaacttc ttcaagaccg
     6061 agatcacgct ggccaacggc gagatccgga agagaccgct catcgagacc aacggcgaga
     6121 cgggggagat cgtgtgggac aagggcaggg atttcgcgac cgtccgcaag gttctctcca
     6181 tgccccaggt gaacatcgtc aagaagaccg aggtccaaac gggcgggttc tcaaaggagt
     6241 ctatcctgcc taagcggaac agcgacaagc tcatcgccag aaagaaggac tgggacccaa
     6301 agaagtacgg cgggttcgac agccctaccg tggcctactc ggtcctggtt gtggcgaagg
     6361 ttgagaaggg caagtccaag aagctcaaga gcgtgaagga gctcctgggg atcaccatca
     6421 tggagaggtc cagcttcgag aagaacccaa tcgacttcct ggaggccaag ggctacaagg
     6481 aggtgaagaa ggacctgatc atcaagctcc cgaagtactc tctcttcgag ctggagaacg
     6541 gcaggaagag aatgctggct tccgctggcg agctccagaa ggggaacgag ctcgcgctgc
     6601 caagcaagta cgtgaacttc ctctacctgg cttcccacta cgagaagctc aagggcagcc
     6661 cggaggacaa cgagcaaaag cagctgttcg tcgagcagca caagcattac ctcgacgaga
     6721 tcatcgagca aatctccgag ttcagcaagc gcgtgatcct cgccgacgcg aacctggata
     6781 aggtcctctc cgcctacaac aagcaccggg acaagcccat cagagagcaa gcggagaaca
     6841 tcatccatct cttcaccctg acgaacctcg gcgctcctgc tgctttcaag tacttcgaca
     6901 ccacgatcga tcggaagaga tacacctcca cgaaggaggt cctggacgcg accctcatcc
     6961 accagtcgat caccggcctg tacgagacga ggatcgacct ctcacaactc ggcggggata
     7021 agagacccgc agcaaccaag aaggcagggc aagcaaagaa gaagaagtga ctcgagatat
     7081 gaagatgaag atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt
     7141 ttttttcttg gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa
     7201 gatcacatta taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat
     7261 ctcttctgca gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa
     7321 gataaggagc tccggtgacg gacgaccaag cccgttattc tgacagttct ggtgctcaac
     7381 acatttatat ttatcaagga gcacattgtt actcactgct aggagggaat cgaactagga
     7441 atattgatca gaggaactac gagagagctg aagataactg ccctctagct ctcactgatc
     7501 tgggtcgcat agtgagatgc agcccacgtg agttcagcaa cggtctagcg ctgggctttt
     7561 aggcccgcat gatcgggctt ttgtcgggtg gtcgacgtgt tcacgattgg ggagagcaac
     7621 gcagcagttc ctcttagttt agtcccacct cgcctgtcca gcagagttct gaccggttta
     7681 taaactcgct tgctgcatca gacttgacgg acacgagcac catgggtttt agagctagaa
     7741 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     7801 ctttttttcc ggcgagcttt gggtacgtca cgtggctcga gcgcgtagtc ctcggtaata
     7861 tcgcagaaca aaagtacctg atatcgagtg tacttcaagt cacaccggcg agtgccagcc
     7921 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     7981 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     8041 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     8101 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     8161 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     8221 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     8281 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     8341 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     8401 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     8461 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     8521 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     8581 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     8641 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     8701 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     8761 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     8821 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     8881 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     8941 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     9001 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     9061 cctcgagc
//
