LOCUS       sequence-182765-       12419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral 
            Replication: WDV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 12419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_223
FEATURES             Location/Qualifiers
     source          1..12419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3379)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2746..2766)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3570..3591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3606..3611,3612..3629,3630..3636)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3644..3660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3649..3671
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3668..3684
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3668..3684
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4142..4266
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4291..4296,4297..4314,4315..4321)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4375..5055
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5375..5680
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5559..5578
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5721..5845)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    7649..7673
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8974..9603
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10032..11105
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11171..11365
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11601..11623)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11709..11849
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11764..11783
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11864..11881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12035..12419,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12115..12134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagttcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgttcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3601 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gtaaggtagt gaacagaagt
     3721 ccggcaggtc cttagcgaaa aaacggggtg tgccagaaaa ctctatcctc taccctgcgt
     3781 ggaggtgtga attctgcaca ctgcaaatgc aatgtgtcca atgctttata tagggcaggt
     3841 tttggcggga gaacagggcc ctagtgttcc cacggtagcg tagcgaatcg tgtgggccct
     3901 gttcggtgtg cggtcggggg gcctccacgc gggttataat attaccccgc gtggtggccc
     3961 ccgacgcgca ctcggctttt cgtgagtgcg cggaggcttt tggaccacat cttttctgat
     4021 cactttcgtg gaagatgttg atttatcaca cttttgacgg ggaaatctgt gccatgcctt
     4081 agcttataag gaagtgcgtg gtagcccatc tcgacaagtt tgtaccgatc aaaagcaggt
     4141 gacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     4201 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     4261 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
     4321 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
     4381 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     4441 gaggcatttc agtcagtcgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     4501 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     4561 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
     4621 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     4681 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     4741 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     4801 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     4861 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     4921 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     4981 agaatgctta atgaattaca acagtactgc gatgagtggc aggcggggcg taatctagag
     5041 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     5101 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     5161 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     5221 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     5281 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     5341 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     5401 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     5461 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga
     5521 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     5581 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     5641 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     5701 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     5761 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     5821 gttcagcttt cttgtacaaa gtggtcacct gcaaaaagtg tttgatcgcc ggcggtacaa
     5881 agtggttaaa ataatatttt atttatctca tgtcattcga ttacagaggc tcggctacga
     5941 gcaaagacaa accaaatata acaaacaaca acccttacac aatgacatcg gaaaacgaaa
     6001 tacaacaccc tgagatatta catttataga aactgtacgc cgtccgcgct aggacagtca
     6061 ctgcgaagca gtgacgtctt cgccggaggc gaacgagtag ttgatgaacg tctcgccttc
     6121 atacatgtag tgaacaacag tgttagagta catgtaatcc gactgttcgg gagtcatatc
     6181 cttgagccaa tcttcgtctg gattaactaa aatgatgcaa ggtattccac cccgtatgac
     6241 ctttcgctta ccatattttg gattgaccgt gaagtcacgc tgagccccga cgaagcactt
     6301 ccagttgggt gtgaacttga atggaatgtc gtcgatgata ttatacttgg cgttgacgtc
     6361 atatgttgtg aaatcaacta gactgttata ataattgtgt gtccctagag accttgccca
     6421 ggaagtcttt cctgttctgg ttggcccgca gatgtagatg gacttatgcc tccccggtga
     6481 ctcctggaat aatcgtccat ccactctaag tcagattgcg cttgatccgc aggagtggaa
     6541 gtacaaagga tataggattc gaggcttacg gagtagagat gttcattttt ccagctttca
     6601 atggtctcat ggcaaatgag tgattcggtt ggaaactcag gtgtgtaagt ggcaactggg
     6661 tcaggaaata gatggcgtgc cgtgtactcg aagtctttga gacggataga ccattcaaac
     6721 ggaaaacgat tgcaaaccat gctgaggaat tcctcgcgag aggaactaga ttcaatgatc
     6781 tgtttcatat ccgcatcacg gtctttacga cctggagttg aaacagccac gaatgttccc
     6841 cactcagctg tgtttacatc ggagtcaacc tccttcgtga tgtaatcacg aacttggttg
     6901 cagtctttgg cagcttgtat atttggatgg aatatggaga atggagatgt atccatacgg
     6961 aggtttaagg cattgggatt ggtgatggaa gcacgaagct tgttctgcac gagaacgtgc
     7021 agatgtggtg atccatcttc gtggagctct ctaacagcag cgatgtagag gggctcatat
     7081 ttgttcaaga gagtgcgaag tgaatccaag gcgtactgtg gctcaagggt acattgagga
     7141 tatgttagaa agaggtactt ggaatagaca cggaacctgg gtgcagatga agaggccatg
     7201 gtagtgaaca gaagtccggc aggtccttag cgaaaaaacg gggtgtgcca gaaaactcta
     7261 tcctctaccc tgcgtggagg tgtgaattct gcacactgca aatgcaatgt gtccaatgct
     7321 ttatataggg caggttttgg cgggagaaca gggccctagt gttcccacgg tagcgtagcg
     7381 aatcgtgtgg gccctgttcg gtgtgcggtc ggggggcctc cacgcgggtt ataatattac
     7441 cccgcgtggt ggcccccgac gcgcactcgg cttttcgtga gtgcgcggag gcttttggac
     7501 cacatctttt ctgatcactt tcgtggaaga tgttgattta tcacactttt gacggggaaa
     7561 tctgtgccat gccttagctt ataaggaagt gcgtggtagc ccatctcggg gccctcgatt
     7621 cgacgttcct gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     7681 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     7741 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
     7801 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     7861 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     7921 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     7981 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     8041 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     8101 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     8161 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     8221 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     8281 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     8341 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     8401 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     8461 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     8521 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     8581 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     8641 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     8701 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     8761 gccgcccgcg cacggctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     8821 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     8881 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     8941 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     9001 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     9061 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     9121 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     9181 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     9241 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     9301 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     9361 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     9421 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     9481 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     9541 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     9601 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     9661 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     9721 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     9781 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     9841 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     9901 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     9961 ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc
    10021 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
    10081 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
    10141 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
    10201 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
    10261 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
    10321 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
    10381 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
    10441 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
    10501 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
    10561 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
    10621 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
    10681 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
    10741 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
    10801 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
    10861 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
    10921 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
    10981 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
    11041 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
    11101 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
    11161 gcctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
    11221 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
    11281 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
    11341 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagcccaa gagctgcaaa
    11401 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
    11461 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
    11521 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
    11581 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggaaacggt cacagcttgt
    11641 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    11701 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
    11761 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    11821 gatgcgtaag gagaaaatac cgcatcaggc cctcttccgc ttcctcgctc actgactcgc
    11881 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    11941 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    12001 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    12061 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    12121 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    12181 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    12241 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    12301 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    12361 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtat
//
