LOCUS       sequence-182781-        9298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Gal4/UAS expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Zhou W, Pal AS, Hsu AY, Gurol T, Zhu X, Wirbisky-Hershberger SE, 
            Freeman JL, Kasinski AL, Deng Q
  TITLE     MicroRNA-223 Suppresses the Canonical NF-kappaB Pathway in Basal 
            Keratinocytes to Dampen Neutrophilic Inflammation.
  JOURNAL   Cell Rep. 2018 Feb 13;22(7):1810-1823. doi: 
            10.1016/j.celrep.2018.01.058.
  PUBMED    29444433
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.01"; journalName: "Cell Rep"; date: 
            "2018-02-13- 13"; volume: "22"; issue: "7"; pages: "1810-1823"
COMMENT     Sequence Label: Tol2-lyzC-Gal4-Vp16-Crys-CFP
FEATURES             Location/Qualifiers
     source          1..9298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1710..1734
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1736..1752
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1762..1780
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1801..2241
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    2492..2626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2723..2741)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(2770..2794)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3445..3478)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      3631..3640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3637..3639,3640..3642,3643..4356)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3943..3962)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4290..4311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4385..4405)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4413..4429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4445..4579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(5018..5205,5206..5217)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5251..5267)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5251..5267)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(5304..5322)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5343..5359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5356..5378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5367..5383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5391..5397,5398..5415,5416..5421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5436..5457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5574..5591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5745..6333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5825..5844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6504..7295,7296..7364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7127..7146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7365..7469)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7632..7653)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(8002..8013,8014..8176)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     8549..8565
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8585..8605
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caggtccttg tgtccttcta aggtctgttt gtggtttaag ctgtttgttg acacacgctt
       61 gtcttctcca tttttctgtg aagttattgt cagtttattg cacactgggt tgcattaagc
      121 tgcagttttt tcacaacagt tttccacatt gtaacagtca gtctccaaat tattccatca
      181 gtcctcaaat ttagtcattt gaggccatga aaacacttaa aactacatga aggtagtttg
      241 gcattaggtt ttagacttgt tcttgtttgg catattgcaa cctgtgtaaa aatgatttga
      301 tttttatttt tttttttttt ttttcagaaa gaaacggtcc aaaaagagtt atgtgaacat
      361 ttatccatgt gtttaatgtt ttttaaatta aaaaaaaaca tttttattaa accacttgtg
      421 actaaacctt ttttagttat tctattttgt gacgagtctg aatggccaaa gtactcttga
      481 atccaaaatc tttatagtaa aatgtttaat cttacttgca gtgttgggta taatttgtaa
      541 caaagttgaa ttccatatct gctgagtgaa aaagtaaagt aagagattgc tgtaaatatt
      601 taggttattt atttattaat acaatataga aagtaacaaa acacaatgta aaaaatcaat
      661 aaagaaagta aatattaaaa gtaatgataa actaatgatg taaacaatat tagcaacaaa
      721 gtttgtacaa ttcagggagg gtaaagaatt tcacctaata gtcaaactct gtattaaata
      781 atataaaatc tttgttctta gtctgaagag gagactgtga gagaaagctt actggttgtt
      841 aaattagaaa atttgtaact tgcagattaa aatgatttaa ataattttat aaaggccatg
      901 tttgaaacaa atgtaaaaac ataataaatg ttaagaattt cagatattga gattttacaa
      961 tttaaaaaaa gcaataaagt aatagcagag acctacactc ttaaagtaaa ttacccaact
     1021 ctgcttacta gtaacttttg gaatagttac tagtatccat tgaacaaata ctagaatcta
     1081 attaaattgt gtgagtgtcc aatgaaaaga ttcttgtatt taaaacagta ccagtatcta
     1141 tatatgtaca aaatttagtg ctagcaaaat gcaaataaat actactgcat ataatggatt
     1201 gcattgtaaa tcgactcgcc ataatacatt tatttcctat ttttactttt aacaaaaaat
     1261 aagttcactg agtatttcat gtcattaaat cgcattaaac ctcttgcatg ctttatgtac
     1321 actagtgcag ttcgctaaat gaccaccggt gtcactaata acacgatcgt ttctgaattc
     1381 tgcattaaac gccatttata cgttgctctt attagtaaac aatttctcca tcatgagctc
     1441 gaccagtatt gcatatttca tcctcttaaa tccagagttg tttcttttaa tcactttctc
     1501 ttcatgataa aagcagtttt tggttttcag aagtttacaa caaacccagt cagttattgc
     1561 ctcagcaaac actggagcaa ctgcacactt gtgaaaagct tcttgcgaaa cacaaacctt
     1621 tctctggact tataaataaa ggcattcagc agctccacca tacacctcca ctggtcttga
     1681 taaagggatc ctttaagggc gaattcgacc caagtttgta caaaaaagca ggcttgtaaa
     1741 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgccacc
     1801 atgaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
     1861 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac
     1921 tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg
     1981 ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt
     2041 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
     2101 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
     2161 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
     2221 caaagacagt tgactgtatc ttcgaggtcg accccgggaa ttcagatctc tcgagccgcc
     2281 cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg
     2341 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg
     2401 ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat gtagctcgag
     2461 cctctagaac tatagtgagt cgtattacgt agatccagac atgataagat acattgatga
     2521 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2581 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2641 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc
     2701 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     2761 ggtcatagca cccagctttc ttgtacaaag tggaccgtcg agcaattggt gctatcatca
     2821 tgacaaagag agaagaaatc agttattaga aatgagttat taaaactatt atgattagaa
     2881 atatgtttct ttctcttaaa caggatttaa ggggaaaata tacagggagc gaaaaatttt
     2941 gacttcatct gtatatacat ataataaata atacatcaat aaattccatc taaatacgct
     3001 accatatata tatatatatg tatatacaca cacacacaca caccatctaa tatatgtcat
     3061 gctacattat taacttcagt atgaaatcta ctctgctctg gagtgtatca gcgagcgact
     3121 cctctgctcg gattatcatc acgtgacctc tgacctgaac acagacctct cttagccata
     3181 ggtatacagc ctatagcata gagctcgggc tgagagggaa agtgccgccg tcagggcttt
     3241 ccattgacag taaaatgctt acggccttct gggcgatgcg atgaccctgc gcttcggcac
     3301 ggctgaactc tgatgacggc gtgtttctat tgtccgccgc gcgccgctgt gctgcccttg
     3361 ccagcgtata aaagctgcgg cagtggtgag gaaggccaga gcgagccagc cttcccagca
     3421 cagctgtagt ttaaacgcgt tgccataact tcgtatagga tactttatac gaagttatct
     3481 gcatgctagg ttgccgggaa cggtgcattg gaacgcgcat tccccgtgtt aattaacagg
     3541 taagtgtctt cctcctgttt ccttcccctg ctattctgct caaccttcct atcagaaact
     3601 gcagtatctg tatttttgct aggggatccc gccaccatgg tgagcaaggg cgaggagctg
     3661 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3721 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3781 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
     3841 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3901 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3961 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4021 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc
     4081 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     4141 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4201 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     4261 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4321 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccaaacccg ctgatcagcc
     4381 tcgacaactt tattatacat agttgataat tcactggccg tcgttttacg gtaccatcga
     4441 tgatgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     4501 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     4561 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     4621 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     4681 tgatcctcta gatcagatct gcgaagatac ggccacgggt gctcttgatc ctgtggctga
     4741 ttttggactg tgctgctcgc agctgctgat gaatcacata cttcctccat tttcttccac
     4801 tgattgactg ttataatttc cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag
     4861 atgtgacatg acgtcacttc caaaggacca atgaacatgt ctgaccaatt tcatataatg
     4921 tgaaaacgat tttcataggc agaataaata acatttaaat taaactgggc atcagcgcaa
     4981 ttcaattggt ttggtaatag caagggaaaa tagaatgaag tgatctccaa aaaataagta
     5041 ctttttgact gtaaataaaa ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt
     5101 aattaagtaa aagtaaaagt attgattttt aattgtactc aagtaaagta aaaatcccca
     5161 aaaataatac ttaagtacag taatcaagta aaattactca agtactttac acctctggtt
     5221 cttgaccccc taccttcagc aagcccagca gatccactag ttctagagcg gccgccaccg
     5281 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     5341 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5401 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5461 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5521 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5581 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6361 tctgacgctc agtggaacga aaactcacgt taaggcattt tggtcatgag attatcaaaa
     6421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6481 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6541 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6601 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6661 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6721 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6781 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6841 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6901 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6961 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7021 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7081 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7141 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7201 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7261 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7321 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7381 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7441 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     7501 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     7561 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     7621 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     7681 aagggcgaaa aaccgtctat cagggcgatg gcccacgctg gctaagaact catcagcctc
     7741 cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg
     7801 tgccattcct tcctctttat aggaatggag actacctcct gtccaacaat gctcttggat
     7861 acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc
     7921 tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga
     7981 ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga
     8041 aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc
     8101 ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta
     8161 aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca
     8221 ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa
     8281 actataagac agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt
     8341 taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct
     8401 gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt
     8461 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
     8521 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
     8581 ctatcaactt tgtatagaaa agttggctcc gaattcgccc ttaaaactcg agagtttgac
     8641 ttgggttgat tatagcaaat agtggaacac aaacagattt tgaaatattc ttgcaaattt
     8701 atttattaaa tccattcaaa tattgtcatt ttttgtactg tgtaatttat attgatttgc
     8761 tcatacaata tttacatttc gcttaaacaa tacactatta tttatattta aaaatagcta
     8821 tatataaatc aaatgtatag ctctttttta cacaattaat caaagctaaa atatttgtca
     8881 cacaaataca gtaagccact tcacaatgat atcttcaaaa tctgtgcttg actgaatcat
     8941 ttcatttatt gttttgtaat tcacattata ccttacgttc tccaggcaca gtttgaattg
     9001 ttgataaatg aagataatac atctgattta attagtctcc tttgtttata ataactttaa
     9061 tctaatttga tagtaataat tccagcaata catccattac aataaaaatc gcttttttat
     9121 tttgcacatt agtttgccgg tatttgttta aagtttcacc atacttttaa atcatgtgct
     9181 atttgaaaag ggctgttatg actggaatta aatgtttttt tttatatcag tgttttagat
     9241 tgaagtacaa tatttatagg taaaattcac cagcatatgc ttaaagatag ccatgctc
//
