LOCUS       sequence-182797-        9142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Photo-activatable/light sensitive Vav2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, 
            Dokholyan NV, Hahn KM
  TITLE     Engineering extrinsic disorder to control protein activity in living
            cells.
  JOURNAL   Science. 2016 Dec 16;354(6318):1441-1444.
  PUBMED    27980211
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-12-16"; volume: "354(6318)"; pages: "1441-1444"
COMMENT     Sequence Label: pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    join(1399..1417,1418..1440,1441..1459,1460..1482,1483..1501,
                     1502..1524,1525..1543,1544..1566,1567..1585,1586..1608,
                     1609..1627,1628..1650,1651..1669)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        1702..1740
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1737..1761
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1856..1865
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1862..2569
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2007..2025)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2275..2294
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2666..3094
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     protein_bind    5556..5572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        5605..6104
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5640..5663)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6037..6056
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6127..6726
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6127..6146)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6623..6643
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7235..7252)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7406..7994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7486..7505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8154..8945,8946..9014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8777..8796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9015..9119)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccac gaggcccttt
     1381 cgtcgtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     1441 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     1501 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     1561 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     1621 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     1681 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
     1741 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     1801 agacaccggg accgatccag cctccgcggc cccagatccg taaagtgaat tcgccgccac
     1861 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     1921 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1981 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     2041 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     2101 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     2161 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     2221 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     2281 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     2341 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     2401 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     2461 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     2521 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt ccggaatgga
     2581 gcagtggcgg caatgcggcc gctggctcat cgactgcaag gtcctgccgc ccaaccaccg
     2641 cgtcgtgtgg ccctcgggat caggtttggc tactacactt gaacgtattg agaagaactt
     2701 tgtcattact gacccaagat tgccagataa tcccattata ttcgcgtccg atagtttctt
     2761 gcagttgaca gaatatagcc gtgaagaaat tttgggaaga aactgcaggt ttctacaagg
     2821 tcctgaaact gatcgcgcga cagtgagaaa aattagagat gccatagata accaaacaga
     2881 ggtcactgtt cagctgatta attatacaaa gagtggtaaa aagttctgga acctctttca
     2941 cttgcagcct atgcgagatc agaagggaga tgtccagtac tttattgggg ttcagttgga
     3001 tggaactgag catgtccgag atgctgccga gagagaggga gtcatgctga ttaagaaaac
     3061 tgcagaaaat attgatgagg cggcaaaaga acttggcagt ggtgcggtgg tcttcgacct
     3121 ggcgcaggcg ctgcgcgacg gcgtccttct gtgccagctg ctgcacaacc tctcccccgg
     3181 ttccatcgac cttaaggaca tcaacttccg gccgcagatg tcccagttct tgtgtctgaa
     3241 gaatattcgc accttcctca aagtctgcca tgacaaattt ggactaagaa acagtgagct
     3301 gtttgaccct ttcgacctct ttgatgtccg agacttcggg aaggtcatct ctgcagtgtc
     3361 ccgtctgtcc ctgcacagca tcgcacagag caaagggatc aggccatttc catcagagga
     3421 gacagctgaa aatgatgacg acgtctaccg gagcctggaa gagctggctg acgagcatga
     3481 cctgggtgag gacatctacg actgtgtccc gtgtgaagat gaaggagatg acatttatga
     3541 ggacatcatc aaggtggagg tgcagcagcc catgaaaatg ggcatgactg aggacgacaa
     3601 gagaagctgc tgcttgttag agattcagga gaccgaggcc aagtactacc gcaccctgga
     3661 ggacattgag aagaactaca tgggtccctt gcggctggtg ctgagcccgg cggatatggc
     3721 tgctgtcttc atcaacctgg aggacctcat caaggtgcat cacagctttc tgcgagccat
     3781 cgatgtgtcc atgatggctg gtggcagtac cctggctaag gtctttctgg agtttaagga
     3841 aaggctcctg atctatggag agtactgtag ccacatggaa cacgctcaga gtacactgaa
     3901 ccagctcctc gccagccgag aggacttcag gcagaaagtg gaggagtgca cactcagggt
     3961 tcaggatggc aagttcaagc tgcaagacct gctggtggtg cccatgcaac gggtgctgaa
     4021 gtaccacctg ctgctcaagg agctcctgag ccattctgca gaccgaccag aaagacaaca
     4081 gctcaaagaa gccctggaag ccatgcagga cttggccatg tacatcaatg aagtgaagcg
     4141 ggacaaggag accttgaaga agattagcga gttccagtgc tccatagaaa acctgcaagt
     4201 gaagctggag gaatttggga ggccaaagat tgacggggag cttaaagtcc ggtccatagt
     4261 caaccacacc aagcaagaca ggtacctgtt cctatttgac aaggtggtca tcgtgtgcaa
     4321 gaggaagggc tacagctatg agctgaagga ggtcattgag ctgctcttcc acaagatgac
     4381 cgatgacccg atgcacaaca aggacatcaa gaagtggtcc tatggcttct acctgattca
     4441 cctccaagga aagcaaggct ttcagttctt ctgcaagacg gaagacatga agcggaagtg
     4501 gatggagcag ttcgagatgg ccatgtcaaa catcaagcca gataaggcca atgccaacca
     4561 tcatagcttc cagatgtaca cattcgacaa gactaccaac tgcaaagcct gcaagatgtt
     4621 tctcaggggt accttctacc agggatacct gtgtaccaga tgtggcgtcg gggcacacaa
     4681 ggaatgcctg gaggtgatcc ccccctgcaa gatgagttca cctgcagatg tggatgctcc
     4741 tggagcagga ccaggtccca agatggttgc tgtgcagaat taccatggta acccagcccc
     4801 tcctgggaag cctgtgttga ccttccagac aggagacgtg atagaactgc tccggggtga
     4861 ccccgattct ccttggtggg aagggcggct ggttcaaacc aggaagtcag ggtattttcc
     4921 cagctcatct gtgaagccct gcccagtgga tggaaggccg cctactggcc gcccaccatc
     4981 ccgggagatc gattacactg cctacccgtg gtttgctggc aacatggaac ggcagcagac
     5041 ggacaatctg ctcaaatctc atgccagtgg gacctacctc atcagagagc gtccagcaga
     5101 ggcagaacgc ttcgccatca gcatcaagtt caatgatgag gtgaaacata tcaaggtggt
     5161 ggagaaagat agctggatcc acatcacgga agctaagaag tttgagagcc tcctggagct
     5221 ggtggagtac taccagtgtc actcactcaa ggaaagcttc aagcagttgg acactacact
     5281 caagttcccc tacaagtctc gggagcgaac caccagcagg gcctcaagcc ggtctccagt
     5341 gttcacaccg cgggtcatcg gcacagccgt ggccaggtac aactttgctg ctcgagacat
     5401 gcgggagctg tcactgcgag aaggtgatgt ggtgaagatc tacagccgaa ttggtgggga
     5461 ccagggctgg tggaaaggag agacaaatgg gcggatcggt tggtttcctt cgacgtacgt
     5521 ggaagaggaa ggcgtccagt gagtcgagtg tggaattgtg agcggataac aatttgtcga
     5581 gggcccctgc aggtcaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     5641 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     5701 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     5761 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     5821 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     5881 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     5941 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     6001 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     6061 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agccaataag
     6121 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6181 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6241 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6301 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6361 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6421 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6481 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6541 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6601 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6661 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6721 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     6781 tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     6841 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     6901 ctgagaatag agaagttcag atcaaggtag gagatccctg agcccacaac ccctcactcg
     6961 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     7021 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     7081 actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt
     7141 tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga
     7201 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7261 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7321 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7381 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7441 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7501 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7561 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7621 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7681 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7741 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7801 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7861 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7921 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7981 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8041 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8101 ttttgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8161 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8221 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8281 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8341 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8401 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8461 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8521 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8581 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8641 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8701 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8761 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8821 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8881 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8941 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9001 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9061 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9121 acatttcccc gaaaagtgcc ac
//
