LOCUS       sequence-182805-       10166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Poliner E, Takeuchi T, Du ZY, Benning C, Farre EM
  TITLE     Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR 
            System in the Oleaginous Microalga, Nannochloropsis oceanica 
            CCMP1779.
  JOURNAL   ACS Synth Biol. 2018 Apr 20;7(4):962-968. doi: 
            10.1021/acssynbio.7b00362. Epub 2018 Mar 14.
  PUBMED    29518315
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00362"; journalName: "ACS Synth Biol"; date: 
            "2018-04-20- 20"; volume: "7"; issue: "4"; pages: "962-968"
COMMENT     Sequence Label: pNOC-CRISPR-compact
FEATURES             Location/Qualifiers
     source          1..10166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..520
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        557..661
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(662..730,731..1522)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(880..899)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1693..2281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2182..2201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      2358..2367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3048..3068)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3073..3082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3084..3110)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3663..7763)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3663..7763)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3663..7763)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(7764..7784)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8005..8016)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_RNA        9007..9082
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           9087..9154
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
ORIGIN
        1 accggtttct tagacggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga
       61 tggaataatt tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt
      121 agaaagtaaa taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt
      181 ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga
      241 ttaaatagat atacattcga ttaacgataa gtaaaatgta aaatcacagg attttcgtgt
      301 gtggtcttct acacagacaa gatgaaacaa ttcggcatta atacctgaga gcaggaagta
      361 caagataaaa ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac
      421 aaaaactatt ttttctttaa tttctttttt tactttctat ttttaattta tatatttata
      481 ttaaaaaatt taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac
      541 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      601 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      661 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      721 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      781 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      841 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      901 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      961 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1021 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1081 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1141 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1201 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1261 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1321 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1381 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1441 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1501 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1561 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1621 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1681 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1741 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1801 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1861 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1921 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1981 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2041 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2101 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2161 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2221 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2281 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2341 gtttaaacac tagtgcggcc gccatggcct ctagtggatc agcttgcatg cctgcaggag
     2401 acgatatcac ctcttctgtt tccacgataa aaatagactg ctcatttctt cgtcgtcttc
     2461 atcgtctgct ttttctgctt cgcctctgtc tggggtctga aaccactaca cacacacaca
     2521 acactcgtac tcccactttc acaaaagcgt aagctcaccg gcttttctta cacgtacatt
     2581 ttagtggatc ccatcacgcc actaccacgc ccgcggggga tggaacggag gggagagaga
     2641 gaagggggaa gcatggatga atgagacatt gagggaaagg aggggaggga gcagtccatc
     2701 agggcgctac ctctcttgtc ccccaaaccc tgttgagccg ttcaacatgt ttcatgtttc
     2761 ctccctcccc ccttccctcc ctggcctttc cgcggagcca ttcaagtgac gtctggaccg
     2821 caccgtaaca aaatcgtttc tatggggggt ttgtttgaca accacgtctt cagcgttttt
     2881 aaaaaaaaaa gcgggcaagc cctctcaccc tcactcatgc ccatcctcct cctctcctgc
     2941 ggaacattct tacaaaaggc gtaactcgac gacaactcaa agaacgacaa acatcaatcc
     3001 caaaaaaaaa atctctactc gtctctcttg gatctttcca attgtcagac cttcctcttc
     3061 tttttcgggc tagccaccat ggacgcgtag tcgggcacgt cgtaggggta tccggccagg
     3121 atgcgctcgc acaggcgcca gccggttacc ccgttgatgg tcacgcggaa caacagcgag
     3181 ccatccgggt tgatcagccg ctcgtcaatg atcttattgc cgttccacag ggtgccggtc
     3241 accgtgatct tcttgccgtc gaacacggca atgccctcgt aaggacggcc gaagtagtca
     3301 atcatgttcg gcgtcacacc gtcgatgacc agcgtcccgt agtgcagtat caccttgaag
     3361 tggtgatcgt ccacggggta cacaaccttg aagatcttct cgatctggcc catttggtcc
     3421 ccgctcaggc cctcgtaggg aatgatgacg tggatgtcga tcttcaggcc gttctcgccg
     3481 ctcaggacga tgcgctggat tggggtcacg gagacgccca ggttctggaa aagcgaggac
     3541 acaccgccct gctccagcac ctggtcgagg ttatagccag ctgtctgccg ccagtcgccc
     3601 acgaagtcct cgagagtaaa caccatgtta acggacccgc tgccggaccc gtcagccctg
     3661 ctgtctccac cgagctgaga gaggtcgatt cttgtttcat agagccccgt aattgactga
     3721 tgaatcagtg tggcgtccag gacctccttt gtagaggtgt accgctttct gtctatggtg
     3781 gtgtcgaagt acttgaaggc tgcaggcgcg cccaagttgg tcagagtaaa caagtggata
     3841 atgttttctg cctgctccct gatgggctta tccctgtgct tattgtaagc agaaagcacc
     3901 ttatcgaggt tagcgtcggc gaggatcact cttttggaga attcgcttat ttgctcgatg
     3961 atctcatcaa ggtagtgttt gtgttgttcc acgaacagct gcttctgctc attatcttcg
     4021 ggagaccctt tgagcttttc atagtggctg gccagataca agaaattaac gtatttagag
     4081 ggcagtgcca gctcgttacc tttctgcagc tcgcccgcac tagcgagcat tcgtttccgg
     4141 ccgttttcaa gctcaaagag agagtacttg ggaagcttaa tgatgaggtc ttttttgacc
     4201 tctttatatc ctttcgcctc gagaaagtcg atggggtttt tttcgaagct tgatcgctcc
     4261 atgattgtga tgcccagcag ttccttgacg cttttgagtt ttttagactt ccctttctcc
     4321 actttggcca caaccagtac actgtaagcg actgtaggag aatcgaatcc gccgtatttc
     4381 ttggggtccc aatctttttt gcgtgcgatc agcttgtcgc tgttcctttt cgggaggata
     4441 ctttccttgg agaagcctcc ggtctgtact tcggtctttt taacgatgtt cacctgcggc
     4501 atggacagga ccttccggac tgtcgcgaaa tccctaccct tgtcccacac gatttctcct
     4561 gtttctccgt ttgtttcgat aagtggtcgc ttccgaatct ctccattggc cagtgtaatc
     4621 tcggtcttga aaaaattcat aatattgctg taaaagaagt acttagcggt ggccttgcct
     4681 atttcctgct cagactttgc gatcattttc ctaacatcgt acactttata gtctccgtaa
     4741 acaaattcag attcaagctt gggatatttt ttgataagtg cagtgcctac cactgcattc
     4801 aggtaggcat catgcgcatg gtggtaattg ttgatctctc tcaccttata aaactgaaag
     4861 tcctttctga aatctgagac cagcttagac ttcagagtaa taactttcac ctctcgaatc
     4921 agtttgtcat tttcatcgta cttggtgttc atgcgtgaat cgagaatttg ggccacgtgc
     4981 ttggtgatct ggcgtgtctc aacaagctgc cttttgatga agccggcttt atccaactca
     5041 gacaggccac ctcgttcagc cttagtcaga ttatcgaact tccgttgtgt gatcagtttg
     5101 gcgttcagca gctgccgcca ataatttttc attttcttga caacttcttc tgaggggacg
     5161 ttatcactct tccctctatt tttatcggat cttgtcaaca ctttattatc aatagaatca
     5221 tctttgagaa aagactgggg cacgatatga tccacgtcgt agtcggagag ccgattgatg
     5281 tccagttcct gatccacgta catgtccctg ccgttctgca ggtagtacag gtagagcttc
     5341 tcattctgaa gctgggtgtt ttcaactggg tgttccttaa ggatttggga ccccagttct
     5401 tttataccct cttcaatcct cttcatcctt tccctactgt tcttctgtcc cttctgggta
     5461 gtttggttct ctcgggccat ctcgataacg atattctcgg gcttatgcct tcccattact
     5521 ttgacgagtt catccacgac cttaacggtc tgcagtattc cctttttgat agctgggcta
     5581 cctgcaagat tagcgatgtg ctcgtgaaga ctgtccccct ggccagaaac ttgtgctttc
     5641 tggatgtcct ccttaaaggt gagagagtca tcatggatca actgcatgaa gttccggttg
     5701 gcaaatccat cggacttaag aaaatccagg attgtctttc cactctgctt gtctcggatc
     5761 ccattgatca gttttcttga cagccgcccc catcctgtat atcggcgcct cttgagctgt
     5821 ttcatgactt tgtcgtcgaa gagatgagcg taagttttca agcgttcttc aatcatctcc
     5881 ctatcttcaa acaacgtaag ggtgaggaca atgtcctcaa gaatgtcctc gttctcctca
     5941 ttgtccagga agtccttgtc tttaatgatt ttcaggagat cgtgatacgt tcccagggat
     6001 gcgttgaagc gatcctccac tccgctgatt tcaacagagt cgaaacattc aatctttttg
     6061 aaatagtctt ctttgagctg tttcacggta actttccggt tcgtcttgaa gaggaggtcc
     6121 acgatagctt tcttctgctc tccagacagg aatgctggct ttctcatccc ttctgtgacg
     6181 tatttgacct tggtgagctc gttataaact gtgaagtact cgtacagcag agagtgttta
     6241 ggaagcacct tttcgttagg cagattttta tcaaagttag tcatcctttc gatgaaggac
     6301 tgggcagagg cccccttatc cacgacttcc tcgaagttcc agggagtgat ggtctcttct
     6361 gatttgcgag tcatccacgc gaatctggaa tttccccggg cgagggggcc tacatagtag
     6421 ggtatccgaa atgtgaggat tttctcaatc ttttccctgt tatctttcaa aaaggggtag
     6481 aaatcctctt gccgcctgag gatagcgtgc agttcgccca ggtgaatctg gtgggggatg
     6541 cttccattgt cgaaagtgcg ctgtttgcgc aacagatctt ctctgttaag ctttaccagc
     6601 agctcctcgg tgccgtccat tttttccaag atgggcttaa taaatttgta aaattcctcc
     6661 tggcttgctc cgccgtcaat gtatccggcg tagccatttt tagactgatc gaagaaaatt
     6721 tccttgtact tctcaggcag ttgctgtctg acaagggcct tcagcaaagt caagtcttgg
     6781 tggtgctcat catagcgctt gatcatacta gcgctcagcg gagctttggt gatctccgtg
     6841 ttcactcgca gaatatcact cagcagaatg gcgtctgaca ggttctttgc cgccaaaaaa
     6901 aggtctgcgt actggtcgcc gatctgggcc agcagattgt cgagatcatc atcgtaggtg
     6961 tctttgctca gttgaagctt ggcatcttcg gccaggtcga agttagattt aaagttgggg
     7021 gtcagcccga gtgacagggc gataagatta ccaaacaggc cgttcttctt ctccccaggg
     7081 agctgtgcga tgaggttttc gagccgccgg gatttggaca gcctagcgct caggattgct
     7141 ttggcgtcaa ctccggatgc gttgatcggg ttctcttcga aaagctgatt gtaagtctga
     7201 accagttgga taaagagttt gtcgacatcg ctgttgtctg ggttcaggtc cccctcgatg
     7261 aggaagtgtc cccgaaattt gatcatatgc gccagcgcga gatagatcaa ccgcaagtca
     7321 gccttatcag tactgtctac aagcttcttc ctcagatgat atatggttgg gtacttttca
     7381 tggtacgcca cctcgtccac gatattgcca aagattgggt ggcgctcgtg ctttttatcc
     7441 tcctccacca aaaaggactc ctccagccta tggaagaaag agtcatccac cttagccatc
     7501 tcattactaa agatctcctg caggtagcag atccgattct ttctgcgggt atatctgcgc
     7561 cgtgctgttc ttttgagccg cgtggcttcg gccgtctccc cggagtcgaa caggagggcg
     7621 ccaatgaggt tcttctttat gctgtggcga tcggtattgc ccagaacttt gaattttttg
     7681 ctcggcacct tgtactcgtc cgtaatgacg gcccagccga cgctgtttgt gccgatatcg
     7741 agcccaatgg agtacttctt gtccaccttc cgctttttct tgggcatgct cgagggttgc
     7801 gtgtgtatct gtgtgcagtg gatattgtta ccgagtttgg tgagcgtgag tccgttagtg
     7861 ccctggtggt ggtggattag gagagtgggt gactcggtgt ccatggcttt cttcgctcat
     7921 tataggaggg gaaaggaatg agggagggtg gggagaccgc gtctgttgtt gaccaccgat
     7981 ttacttcttg cctcccttcc cctccctccc ctcaatccgt acgacacaaa tagtagccga
     8041 gtgtctgctg cagagcgcat gattagtgtg gtagacaacg agggagggaa ggatgtacag
     8101 ggcatggcac ggagaagcga tggtggccag gaagaggaga ggtcgcgaga acaggatgtg
     8161 ttgcgaatgg ataaaaacag aaagcgatgg ctctgggctt cgaaagcagg ggacattagg
     8221 acgtgtagac catctcgacg gatccctctg tatctctgtt gtgcgtgaat gttttctgtg
     8281 cacgtgtagt gtgtgagagt agaacccggg aactcgaaca gagaaaagca tgggtggctg
     8341 tggtgtggag gcttcgttcc caccacatgc ccttctcctt cgcctcgcct ctccctgcct
     8401 tcttccacgc acccttgcgc ccctcgttct caatacctgg ctcacttcca ccattcaaac
     8461 aaccatcacg atacaggcat ttatctatcg ttgaagactt cttcctccgg tagatcttag
     8521 ccaaggtaag aagaggggca tgcagcaagg agaaagaaat gatgcatgat gaggaataga
     8581 aggggaggag ggagggatat gatgggaagc gaaagcgcat attctggtgg tctgctgcct
     8641 gatggggacg cgtctagctg tgacactgag gacggtggct gctggtggct gcgggcgctg
     8701 ccttggtgat caatgggagt aaagggaggg aaggaggtag cgtgaacgga tgacgcggag
     8761 aagtttaggg gtctctttac gtatcgcccc tgccgcccgc ctctctgcga taaatgtgcc
     8821 tgttaccctg cagcctctat tcttcactgt gttcctgttt tccaacagcc tctattcttc
     8881 cctgtctttt gttgcagtgg cgtcatcctc tctttgcccc agtcgtcgtt ctctcgactc
     8941 actcactccc cccctccttc cctccctcca tccacagaat cgatgaagag caggccttgg
     9001 ctcttcgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     9061 aaaagtggca ccgagtcggt gcttttggcc ggcatggtcc cagcctcctc gctggcgccg
     9121 gctgggcaac atgcttcggc atggcgaatg ggactcctgg gtaccatggg aaagaaagga
     9181 tgagaaagga gaaaggacat ctagataacc ggcatatcac ggtggtgtat tagtgtagaa
     9241 tagtgaagag aagacttggg aaaatgtgta ggaaaggttg tttctgtgta tgtgggttgg
     9301 gatgggtggc tgtttgagaa ggaacagcgg gcagggcgat gtagtgctga acgggcacgg
     9361 aaccactgag actgaaggaa gtagggagag agaggggcag gggacgtgca ctttaatctt
     9421 tgcctcggta gagtataccc atgcaagagt atgtggccac ctgtggtggc ttttggccag
     9481 gtctggtgca gtgccaatca tctcccatca ataatacaac ttcagaacaa cggcgcattg
     9541 atggggagag agaaagtaaa tttaagtaag gggtacgtag tagaggattc aactgaaata
     9601 ttttcgagga gcggttggga agttgaccga ttgaaaggag aagggagggg agcaggtgtg
     9661 atagtcatgt gtaaagtaat tcttttttgc cgtcgtcaca caatccacat caatgataaa
     9721 atatgtttaa ggatcaatca cacggagtcg gtcataaggc aaccgcaaac gcaatgcaaa
     9781 ctagcaagca agcaggacca caacaacaac cattaccatc acaggcgaca gcagcagcag
     9841 ctgcagcagc agcaaggcaa gcaaaggcca tctgcgcgtg gacttttcca ggcaccggct
     9901 ttaagcgtaa ttttcaatga ttgttgtccg tgtatcctct caccctttca ccgcttgcgc
     9961 gcacgtgagc agcacaactg gtaatgcccg aggacaatag acgaggaaaa agaagaagaa
    10021 aaatgaagag ggagtgatga agaagaagag aagaataaaa gaagacagaa aagaagataa
    10081 aaaagtcaaa agacgacaaa gacggaaaaa aaagaaacgc gcaattttaa caccagcaac
    10141 gacgacgaaa agggcgtcag ctttgc
//
