LOCUS       sequence-182825-       14378 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in eukaryotic cells and generating stable cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14378)
  AUTHORS   Gao W, Yang J, Liu W, Wang Y, Shao F
  TITLE     Site-specific phosphorylation and microtubule dynamics control Pyrin
            inflammasome activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4857-66. doi: 
            10.1073/pnas.1601700113. Epub 2016 Aug 1.
  PUBMED    27482109
REFERENCE   2  (bases 1 to 14378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1601700113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-08-16- 16"; volume: "113"; issue: "33"; pages: 
            "E4857-66"
COMMENT     Sequence Label: pWPI-IRES-eGFP-MEFV
FEATURES             Location/Qualifiers
     source          1..14378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(222..242)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        227..556
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      407..542
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1690..1755
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1885..1905
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2330..2464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2367..2386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2421..2440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2632..3265
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3312..3437
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3930..4163
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4348..4392
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4775..5953
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5006..5944
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5901..5921
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5983..6006
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    8397..8514
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8563..9130
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8581..9132
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8725..8742)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8952..8971
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9135..9137,9138..9140,9141..9851)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9135..9137,9138..9140,9141..9851)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9180..9201)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9441..9460)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9788..9809
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9930..9946)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9931..9947)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    9960..10548
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10013..10033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10431..10442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(10692..10725)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10745..10925
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10950..10968)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10951..10968)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11258..11277)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11491..11510)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11610..11632
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11670..11688)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11756..11860
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11861..11929,11930..12721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12079..12098)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12892..13480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13381..13400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    13768..13789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13804..13809,13810..13827,13828..13834)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13842..13858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13847..13869
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13866..13882
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13866..13882
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13901..13921
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13903..13921
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        13949..14175
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             14176..14356
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
       61 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      121 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
      181 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
      241 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      301 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      361 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
      421 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
      481 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
      541 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
      601 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
      661 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
      721 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
      781 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
      841 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
      901 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
      961 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     1021 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     1081 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     1141 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     1201 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     1261 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     1321 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     1381 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     1441 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     1501 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     1561 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     1621 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1681 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1741 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     1801 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     1861 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     1921 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     1981 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     2041 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     2101 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     2161 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     2221 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2281 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2341 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2401 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     2461 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     2521 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     2581 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     2641 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     2701 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     2761 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     2821 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     2881 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     2941 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     3001 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     3061 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     3121 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3181 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3241 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     3301 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     3361 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     3421 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     3481 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     3541 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     3601 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     3661 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     3721 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     3781 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     3841 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     3901 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     3961 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     4021 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     4081 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     4141 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     4201 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     4261 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     4321 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     4381 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     4441 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     4501 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     4561 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     4621 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg accatcgatg
     4681 tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     4741 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
     4801 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     4861 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     4921 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     4981 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     5041 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     5101 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     5161 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     5221 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     5281 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     5341 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     5401 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     5461 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     5521 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     5581 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     5641 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     5701 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     5761 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     5821 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     5881 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     5941 tcaggtgtcg tgaggaattt cgacatttaa atttaatcga tggactacaa ggacgacgat
     6001 gacaagttaa ttaagatggc taagacccct agtgaccatc tgctgtccac cctggaggag
     6061 ctggtgccct atgacttcga gaagttcaag ttcaagctgc agaacaccag tgtgcagaag
     6121 gagcactcca ggatcccccg gagccagatc cagagagcca ggccggtgaa gatggccact
     6181 ctgctggtca cctactatgg ggaagagtac gccgtgcagc tcaccctgca ggtcctgcgg
     6241 gccatcaacc agcgcctgct ggccgaggag ctccacaggg cagccattca ggaatattcc
     6301 acacaagaaa acggcacaga tgattccgca gcgtccagct ccctggggga gaacaagccc
     6361 aggagcctga agactccaga ccaccccgag gggaacgagg ggaacggccc tcggccgtac
     6421 gggggcggag ctgccagcct gcggtgcagc cagcccgagg ccgggagggg gctgtcgagg
     6481 aagcccctga gcaaacgcag agagaaggcc tcggagggcc tggacgcgca gggcaagcct
     6541 cggacccgga gcccggccct gccgggcggg agaagccccg gcccctgcag ggcgctagag
     6601 gggggccagg ccgaggtccg gctgcgcaga aacgccagct ccgcggggag gctgcagggg
     6661 ctggcggggg gcgccccggg gcagaaggag tgcaggccct tcgaagtgta cctgccctcg
     6721 ggaaagatgc gacctagaag ccttgaggtc accatttcta caggggagaa ggcgcccgca
     6781 aatccagaaa ttctcctgac tctagaggaa aagacagctg cgaatctgga ctcggcaaca
     6841 gaaccccggg caaggcccac tccggatgga ggggcatctg cggacctgaa ggaaggccct
     6901 ggaaatccag aacattcggt caccggaagg ccaccagaca cggctgcgag tccccgttgc
     6961 cacgcccagg aaggagaccc agttgacggt acctgtgtgc gtgattcctg cagcttcccc
     7021 gaggcagttt ctgggcaccc ccaggcctca ggcagccgct cacctggctg cccccggtgc
     7081 caggactccc atgaaaggaa gagcccggga agcctaagcc cccagcccct gccacagtgt
     7141 aagcgccacc tgaagcaggt ccagctgctc ttctgtgagg atcacgatga gcccatctgc
     7201 ctcatctgca gtctgagtca ggagcaccaa ggccaccggg tgcgccccat tgaggaggtc
     7261 gccctggaac acaagaagaa aattcagaag cagctggagc atctgaagaa gctgagaaaa
     7321 tcaggggagg agcagcgatc ctatggggag gagaaggcag tgagctttct gaaacaaact
     7381 gaagcgctga agcagcgggt gcagaggaag ctggagcagg tgtactactt cctggaacag
     7441 caggagcatt tctttgtggc ctcactggag gacgtgggcc agatggttgg gcagatcagg
     7501 aaggcatatg acacccgcgt atcccaggac atcgccctgc tcgacgcgct gattggggaa
     7561 ctggaggcca aggagtgcca gtcagaatgg gaacttctgc aggacattgg agacatcttg
     7621 cacagggcta agacagtgcc tgtccctgaa aagtggacca ctcctcaaga gataaaacaa
     7681 aagatccaac tcctccacca gaagtcagag tttgtggaga agagcacaaa gtacttctca
     7741 gaaaccctgc gttcagaaat ggaaatgttc aatgttccgg agctgattgg cgctcaggca
     7801 catgctgtta atgtgattct ggatgcagaa accgcttacc ccaacctcat cttctctgat
     7861 gatctgaaga gtgttagact tggaaacaag tgggagaggc tgcctgatgg cccgcaaaga
     7921 tttgacagct gtatcattgt tctgggctct ccgagtttcc tctctggccg ccgttactgg
     7981 gaggtggagg ttggagacaa gacagcatgg atcctgggag cctgcaagac atccataagc
     8041 aggaaaggga acatgactct gtcgccagag aatggctact gggtggtgat aatgatgaag
     8101 gaaaatgagt accaggcgtc cagcgttccc ccgacccgcc tgctaataaa ggagcctccc
     8161 aagcgtgtgg gcatcttcgt ggactacaga gttggaagca tctcctttta caatgtgaca
     8221 gccagatccc acatctatac attcgccagc tgctctttct ctgggcccct tcaacctatc
     8281 ttcagccctg ggacacgtga tggagggaag aacacagctc ctctgactat ctgtccagtg
     8341 ggtggtcagg ggcctgactg aggcgcgccc gattaatctc gacggtatcg gttaactttt
     8401 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     8461 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat
     8521 cacgagacta gcctcgaggt ttaaactacg ggctgcagga attccgcccc ccccccccta
     8581 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     8641 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     8701 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     8761 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     8821 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     8881 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     8941 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     9001 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     9061 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     9121 acgatgataa taccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     9181 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     9241 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     9301 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     9361 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     9421 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     9481 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     9541 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     9601 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     9661 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     9721 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     9781 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     9841 agctgtacaa gtccggactc agatctcgac tagctagtag ctagctagct agtcgagctc
     9901 aagcttcgaa ttcgatatca agcttatcgc gataccgtcg acctcgaggg aattccgata
     9961 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
    10021 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
    10081 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
    10141 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
    10201 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
    10261 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
    10321 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
    10381 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
    10441 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
    10501 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattcg
    10561 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
    10621 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tgggatcaat
    10681 tcaccatggg aataacttcg tatagcatac attatacgaa gttatgctgc tttttgcttg
    10741 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    10801 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    10861 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    10921 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
    10981 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
    11041 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
    11101 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
    11161 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
    11221 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
    11281 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
    11341 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
    11401 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
    11461 aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
    11521 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
    11581 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
    11641 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
    11701 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
    11761 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    11821 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    11881 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    11941 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    12001 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    12061 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    12121 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    12181 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    12241 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    12301 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    12361 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    12421 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    12481 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    12541 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    12601 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    12661 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    12721 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    12781 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    12841 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    12901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    12961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    13021 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    13081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    13141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    13201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    13261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    13321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    13381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    13441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    13501 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    13561 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    13621 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    13681 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    13741 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    13801 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    13861 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    13921 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
    13981 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
    14041 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
    14101 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
    14161 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    14221 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
    14281 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
    14341 tggaaaatct ctagcagtgg cgcccgaaca gggacttg
//
