LOCUS       sequence-182850-        9315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  As a control of miRNA sponge.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Zhou W, Pal AS, Hsu AY, Gurol T, Zhu X, Wirbisky-Hershberger SE, 
            Freeman JL, Kasinski AL, Deng Q
  TITLE     MicroRNA-223 Suppresses the Canonical NF-kappaB Pathway in Basal 
            Keratinocytes to Dampen Neutrophilic Inflammation.
  JOURNAL   Cell Rep. 2018 Feb 13;22(7):1810-1823. doi: 
            10.1016/j.celrep.2018.01.058.
  PUBMED    29444433
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.01"; journalName: "Cell Rep"; date: 
            "2018-02-13- 13"; volume: "22"; issue: "7"; pages: "1810-1823"
COMMENT     Sequence Label: Tol2-krt4-RFP
FEATURES             Location/Qualifiers
     source          1..9315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(16..471)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(153..174)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     365..384
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     598..620
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     613..629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        639..657
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(787..798,799..961)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1333..1353
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3616..3640
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3642..3658
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3668..3686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3724..3733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3730..4437
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKL"
     primer_bind     complement(4456..4472)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4503..4521)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(4550..4574)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4579..4713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4603..4622)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4657..4676
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6854..7041,7042..7053)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7087..7103)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7140..7158)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7179..7195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7179..7195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7192..7214)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7203..7219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7227..7233,7234..7251,7252..7257))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7272..7293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7410..7427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7581..8169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7661..7680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8340..9131,9132..9200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8963..8982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9201..9305)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
       61 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
      121 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
      181 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
      241 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
      301 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
      361 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
      421 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
      481 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      541 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      601 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
      661 aattgggtac aggtcattgc acaacaccag aaatgccctc tgatctgcaa aagacgtgaa
      721 tatctgttca gacacccata tccactctgt tccacacagg tcagaggttt gtccaggagt
      781 tcttgacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta
      841 gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag
      901 taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga
      961 aagaaaacta gagattcttg tttaagcttt taatctcaaa aaacattaaa tgaaatgcat
     1021 acaaggtttt atcctgcttt agaactgttt gtatttaatt atcaaactat aagacagaca
     1081 atctaatgcc agtacacgct actcaaagtt gtaaaacctc agatttaact tcagtagaag
     1141 ctgattctca aaattgttag tgtcaagcct agctcttttg gggctgaaaa gcaatcctgc
     1201 agtgctgaaa agcctctcac aggcagccga tgcgggaaga ggtgtattag tcttgataga
     1261 gaggctgcaa atagcaggaa acgtgagcag agactccctg gtgtctgaaa cacaggccag
     1321 atgggccgat atcaactttg tatagaaaag ttggctccga attcgccctt aaaactcgag
     1381 ctcaagcttc gaattcctac agtaaagctt ctccacaatg tcccggagat ccagcccgga
     1441 catgcatttt cttgcctttc cttcccttct aagtctgacg tccttttaag agcttgtgca
     1501 tgaaagcaaa tttagagctt attactcatc ttaaacaccc atacaaagtg atgattgccg
     1561 taccatgatc tcacaccttt cacacctggt ttatactatg atagttgtag acgattgcgt
     1621 aatgctacta aatgcccatc agtgctggct gtgacaccca actgctgcca tttcgtgttg
     1681 acttgcacga gaaatgagaa attgtctgac tatgcagggt gtactatgcg tgggaactta
     1741 tcagtggtca ttaaatacta tggtttaaag ttagagcaaa gtgtgctgta tttttgtgtc
     1801 agcttagctg ctgttttgtg tgtaaagtaa caaatgacaa atactcaaac tattgaaatt
     1861 aagtagtttt tctcagaaat tgtaatttac taagtagttt aaaaatgtgt acttttactt
     1921 tcccttgagc acatttttag tgcagtattg gtacttttat ttcactactt tccttcaacc
     1981 tgcagtcact actttatttt ttcttgtcta tgtggattag acaaatcagt cctgtgattc
     2041 ctgtccaatc aaattgcaca gaaggtaaat cacatcataa tgaacttcct caagacatgg
     2101 gccatttata attgcagcaa actgtttgcc agaattaaaa gaagatgtca aaaatcttta
     2161 cacgcattaa cccagagact gcttagatgc atgtcactga tgagaagatg acagatgttt
     2221 tctgtatgat gaccgaaata accttaaacg cacacaagac ggcacaagac gtcaacatgg
     2281 ctataggttt acgttgtacc ccattgcaat ggggacgttg tattttgttt agaaatgaaa
     2341 attaggttga cgtcagagct caacgtcagg tcgatgtcaa ggttcaacat ccaatctaaa
     2401 atcatatatc aatgtctaat gatgttacag cttgatgttg tgtggatgtt acccctatga
     2461 tgtctatcag aagttggatt gtggttgcca tacctaatga ataaatgtca ttattttaca
     2521 tttgtttaag atgttggttc gacattggac ttatgtcgct ttccaacaca acctaaatcc
     2581 accaaatatt aacttcctat gacatcgtta ttggacatca aaataacaat atccttagat
     2641 gctggctaga ctttgaattt aggtcaccac aacctatatt taacctaata ttaacatctt
     2701 atgatgttgt gtgcctgctg ggcaataact aaatgcacta cagaatgtta cgtttacaca
     2761 catgtaaatt acatgtaaat gcattagctt tttaagacat aatactcact actaactact
     2821 cttgagtact tttaaaaaag ctacttttta ctcatacttt gagtaacagt tacaacagat
     2881 acttttactc gcactggatt tttaggcatg taatggtatt tttactatga tttttcagta
     2941 ctctttccac tactgcagcc ctccccatac ataatcatat gtttacacat atggtggagt
     3001 ttagagccat aaactacatt aactttgtta gccgccagca ttactgtgca gaattgtgtg
     3061 tgtgcacatt ttccaatatc aatacagaag gaaactctgt tccctgttcc cttgtaaatc
     3121 tcaacaatgc aactgttcag ctcaggggga aaaatgccct gccagatcca aacgctggca
     3181 aaagtggatg gaaaaaagcc tttcattaat gtaagagttg ctgcgcgccc cacccagata
     3241 aaaagagcag aggttaacat gctctctacg gctgtccagc caaccagata ctgaggcaga
     3301 aacacacccg cttgcagatg gtgagagcta cactgtcttt ttcagagttt ctactggaat
     3361 gcctgtcctc aagtctcaag cctctccttg cattttctca ttccacctgg ggcaaagccc
     3421 caggctgggt gtgacaccat ttatcttacc actttctctc tgtacctgtc taacaggtag
     3481 ggtgtgtgtg agagtgcgta tgtgtgcaag tgtgtgtgtg agagcagtca gctccacccc
     3541 ctcaagagtg tgtataaaat tggtcagcca gctgctgaga gacacgcaga gggatccttt
     3601 aagggcgaat tcgacccaag tttgtacaaa aaagcaggct tgtaaaacga cggccagtga
     3661 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga
     3721 ctcgccacca tggtgtctaa gggcgaagag ctgattaagg agaacatgca catgaagctg
     3781 tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag
     3841 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
     3901 gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat caaccacacc
     3961 cagggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagagtc
     4021 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     4081 tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc catccaacgg ccctgtgatg
     4141 cagaagaaaa cactcggctg ggaggccaac accgagatgc tgtaccccgc tgacggcggc
     4201 ctggaaggca gaagcgacat ggccctgaag ctcgtgggcg ggggccacct gatctgcaac
     4261 ttcaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcgtctac
     4321 tatgtggacc acagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag
     4381 cacgaggtgg ctgtggccag atactgcgac ctccctagca aactggggca caaacttaag
     4441 agctgtacaa gtaaggatcc actagttcta gagcggccgc ggtggagctc cagcttttgt
     4501 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagca cccagctttc
     4561 ttgtacaaag ttgatgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     4621 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     4681 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     4741 tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat
     4801 tgggccctcg agcatatgag atctgtcccg atctttctct tctgtgctgt caatttagag
     4861 tagtttttga ggtaatttgg gtgcattctc tgtaaaaaca acaaataaat atttatattg
     4921 tatgcaataa atgtaacaat tacacataca ctctctgatt acagcattaa agggttagtt
     4981 cacccaaaaa tgaaaagatg tcattaatga ctcaccctca tgtcgttcca agcccgtaac
     5041 acctccgttc atcttcggaa cacagtttaa gatattttag atttagtccg agagctttct
     5101 gtccctccac tgaaaatgta tgtacggtat actgtccatg tccagaaagg taataaaaac
     5161 atcaaagtag tccatgtgac atcagtgggt taggtagaat tatttgaagc atcgaaaata
     5221 cattttggtc caaaaataac aaaacctacg actttattca gcattgtagc gtcactgcga
     5281 agtcgtgaac gcggattgac aacagacccg gaagagaata caatgccgaa taaagtcgta
     5341 ggttttgtta tttttggacc aaaatgtatt cgatgcttca aaaaattcta actaacccac
     5401 tgatgtcaca tggactactt tgatgttttt attacctttc tggacatgga cagtataccg
     5461 tacatacatt ctcaatggag gcacagaaag ctctcggact aaatctaaaa tatcttaaac
     5521 tgtgttctga agatgaacgg aggtcttacg ggcttggaac gacatgaggg cgagtcatta
     5581 atgacattat tttcattttt gggtgaacta accctttaat aaaactgaca tttgcagcca
     5641 attgcacact ttataatgtt aaatacttgt tgattttaga aatacagtag catttagtga
     5701 caatcaaaca ttaattttat ttctgattat attgacaaaa acacaggact gtaaggctga
     5761 catgacaggg tgaattgatt tcattacatt agttttgatg gtaacacttt tacaataagg
     5821 ttcgttagtt aactacatta gttaacatga actaataatg aactgcacgt atacatgtta
     5881 atttcaacat ttactaatac tttatatctt gttaacatta gttaatgcac tgtgaactaa
     5941 catgaacaaa caatgaacat ctgtatttct attaactaac gttaacaaag attaacagat
     6001 acagtaacaa atgtattgct catggttatt taatgttagt taatacatga actaatcaac
     6061 ctcattgtaa agtgttaccg ttttgacaat acaacatgtt tggaaaaaaa tttctcgctt
     6121 ggttttcttg tgttttgcat gaattcaaac acaatgagag cacggaatga taatgattat
     6181 tcaaagctgc aataggcgcc aaatttcccg tggacctaac gttaccaatt acaaatgcat
     6241 tttagcattt tattacgtga gccttatatt gtcaatcgca tgcattagtg aaaataggcc
     6301 tactacacat ataaaaggcg cgccatcaac gtcaaaacat gcacacccaa aaaaaaaaaa
     6361 aaaaaaaaaa aaaaaaacta tcagtaaaac aaaatactta atgtacttac ctcaatatgc
     6421 ttccttaggt ttgatggcga acttttgaag gccgatattt ctttattaag cgggagacag
     6481 aggacacatt tcatcttgaa tgaatcttta tttacaccac ttaaagaaaa gaattcgcga
     6541 agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct
     6601 gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta
     6661 atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa
     6721 ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa
     6781 taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag
     6841 ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt
     6901 aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg
     6961 atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat
     7021 caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc
     7081 ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc
     7141 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     7201 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7261 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7321 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7741 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7981 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9301 ccgcgcacat ttccc
//
