LOCUS       sequence-18290-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Lorick KL, Jensen JP, Fang S, Ong AM, Hatakeyama S, Weissman AM
  TITLE     RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent 
            ubiquitination.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Sep 28. 96(20):11364-9.
  PUBMED    10500182
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Sep 28. 96(20):11364-9."
COMMENT     Sequence Label: pcDNA3-HA-AO7/2537
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             141..167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctacta gagaaccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttggtaccga gctcggatcc actagtaacg gccgccagtg
      121 tgctggaatt caccatggcc tacccatacg acgtcccaga ctacgctgcc ggagaagaca
      181 ttgcggcgtc tgcgtcgaca gctgctgggg aagaggactg ggtccttccc tctgaagttg
      241 aagtattaga gtctatctat ctggatgagc tacaggtgat gaaagggaat ggcagatcac
      301 catgggagat ctttatcact ctgcaccctg ccaccgcaga ggtccaagat tcgcagtttg
      361 tctgctttac tctggttctt cggatcccag tccagtatcc ccacgaggtt ccacagatct
      421 ccatccgtaa ccctcgagga ctttcagatg agcagatcca caagatctca caggccctgg
      481 gccatgtggc caaggaagga ctaggcactg ccatgctcta tgaactcatt gagaaaggga
      541 aggagattct cacggataac aacattcccc atggccagtg tgtcatctgc ctctatggtt
      601 tccaggagaa ggaggccttt accaaaacac cctgttacca ctacttccac tgccattgcc
      661 ttgccaggta catccagcac atggaacaag agctgactac acaggaacag gaacaggagc
      721 gacagcacgt tgtcaccaag cagaaggcag tcggtgtgca gtgtcctgtg tgc
//
