LOCUS       sequence-182908-         555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Enhanced red-shifted bioluminescence when paired with 
            diphenylterazine (DTZ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Yeh HW, Karmach O, Ji A, Carter D, Martins-Green MM, Ai HW
  TITLE     Red-shifted luciferase-luciferin pairs for enhanced bioluminescence 
            imaging.
  JOURNAL   Nat Methods. 2017 Sep 4. doi: 10.1038/nmeth.4400.
  PUBMED    28869756
REFERENCE   2  (bases 1 to 555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Sep 4. doi: 10.1038/nmeth.4400."
COMMENT     Sequence Label: pcDNA3-teLuc c-myc
FEATURES             Location/Qualifiers
     source          1..555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..513
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLSQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDNHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLHERILA"
     CDS             523..552
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacttg
       61 agtcaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
      121 actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc
      181 atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag
      241 gtggtgtacc ctgtggataa tcatcacttt aaggtgatcc tgcactatgg cacactggta
      301 atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc
      361 gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc
      421 gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg
      481 accggctggc gtctgcatga acgcattctg gcgggctccg gcgaacaaaa actcatctca
      541 gaagaggatc tgtaa
//
