LOCUS       sequence-18291-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Jensen JP, Bates PW, Yang M, Vierstra RD, Weissman AM
  TITLE     Identification of a family of closely related human ubiquitin 
            conjugating enzymes.
  JOURNAL   J Biol Chem. 1995 Dec 22. 270(51):30408-14.
  PUBMED    8530467
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1995 Dec 22. 270(51):30408-14."
COMMENT     Sequence Label: pET 15b-UbcH5a/2417
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(536..554)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      550..597
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcgctg aagaggattc
       61 agaaagaatt gagtgatcta cagcgcgatc cacctgctca ctgttcagct ggacctgtgg
      121 gagatgactt gttccactgg caagccacta ttatggggcc tcctgatagc gcatatcaag
      181 gtggagtctt ctttctcact gtacattttc cgacagatta tccttttaaa ccaccaaaga
      241 ttgctttcac aacaaaaatt taccatccaa acataaacag taatggaagt atttgtctcg
      301 atattctgag gtcacaatgg tcaccagctc tgactgtatc aaaagtttta ttgtccatat
      361 gttctctact ttgtgatcct aatccagatg accccttagt accagatatc gcacaaatct
      421 ataaatcaga caaagaaaaa tacaacagac atgcaagaga atggactcag aaatatgcaa
      481 tgtaaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      541 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      601 aaaggaggaa ctatatccgg atatcccgca agaggcccgg cagtaccggc ataaccaaac
      661 ctatgcctac agcatccagg gtgac
//
