LOCUS       sequence-18292-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Jensen JP, Bates PW, Yang M, Vierstra RD, Weissman AM
  TITLE     Identification of a family of closely related human ubiquitin 
            conjugating enzymes.
  JOURNAL   J Biol Chem. 1995 Dec 22. 270(51):30408-14.
  PUBMED    8530467
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1995 Dec 22. 270(51):30408-14."
COMMENT     Sequence Label: pET 15b-UbcH5c/2418
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(577..595)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      591..638
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(813..818,819..835,836..841))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttcccctcta gaataatttt gtttaacttt aagaaggaga tataccatgg gcgctgaaac
       61 ggattaataa ggaacttagt gatttggccc gtgaccctcc agcacaatgt tctgcaggtc
      121 cagttgggga tgatatgttt cattggcaag ccacaattat gggacctaat gacagcccat
      181 atcaaggcgg tgtattcttt ttgacaattc attttcctac agactacccc ttcaaaccac
      241 ctaaggttgc atttacaaca agaatttatc atccaaatat taacagtaat ggcagcattt
      301 gtctcgatat tctaagatca cagtggtcgc ctgctttaac aatttctaaa gttcttttat
      361 ccatttgttc actgctatgt gatccaaacc cagatgaccc cctagtgcca gagattgcac
      421 ggatctataa aacagacaga gataagtaca acagaatatc tcgggaatgg actcagaagt
      481 atgccatgtg atgctacctt aaagtcagaa taacctgcat tatagcggat ccggctgcta
      541 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      601 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      661 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
      721 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
      781 gcgttagcaa tttaactgtg ataaactacc gcataaaagc ttatcgatga taagctgtca
      841 aacatg
//
