LOCUS       sequence-18293-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Jensen JP, Bates PW, Yang M, Vierstra RD, Weissman AM
  TITLE     Identification of a family of closely related human ubiquitin 
            conjugating enzymes.
  JOURNAL   J Biol Chem. 1995 Dec 22. 270(51):30408-14.
  PUBMED    8530467
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1995 Dec 22. 270(51):30408-14."
COMMENT     Sequence Label: pET-15b-UbcH5b/2419
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(581..599)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      595..642
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttcccctcta gaataatttt gtttaacttt aagaaggaga tataccatgg ctctgaagag
       61 aatccacaag gaattgaatg atctggcacg ggaccctcca gcacagtgtt cagcaggtcc
      121 tgttggagat gatatgttcc attggcaagc tacaataatg gggccaaatg acagtcccta
      181 tcagggtgga gtatttttct tgacaattca tttcccaaca gattacccct tcaaaccacc
      241 taaggttgca tttacaacaa gaatttatca tccaaatatt aacagtaatg gcagcatttg
      301 tcttgatatt ctacgatcac agtggtctcc agcactaact atttcaaaag tactcttgtc
      361 catctgttct ctgttgtgtg atcccaatcc agatgatcct ttagtacctg agattgctcg
      421 gatctacaaa acagatagag aaaagtacaa cagaatagct cgggaatgga ctcagaagta
      481 tgcgatgtaa ttaaagaaat tattggataa cctctacaaa taaagatagg ggatccggct
      541 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      601 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      661 tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat
      721 ccagggtgac g
//
