LOCUS       sequence-182940-        6676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  leu1 integration plasmid with adh1pr:Z3EV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Ohira MJ, Hendrickson DG, Scott McIsaac R, Rhind N
  TITLE     An estradiol-inducible promoter enables fast, graduated control of 
            gene expression in fission yeast.
  JOURNAL   Yeast. 2017 Aug;34(8):323-334. doi: 10.1002/yea.3235. Epub 2017 Jun 
            8.
  PUBMED    28423198
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3235"; 
            journalName: "Yeast"; date: "2017-08"; volume: "34"; issue: "8"; 
            pages: "323-334"
COMMENT     Sequence Label: pFS461
FEATURES             Location/Qualifiers
     source          1..6676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2003..2744
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     primer_bind     complement(4189..4209)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4189..4207)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4228..4244)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4228..4244)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4241..4263)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4252..4268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4276..4282,4283..4300,4301..4306))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4321..4342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4459..4476)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4630..5218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4710..4729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5389..6180,6181..6249))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6012..6031
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6250..6354)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6422..6440
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6478..6500)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6600..6619
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtttcccgaa accaggcatt tttgcggtag ggtctgaaac tttcacgaaa ccatttcaca
       61 acaaaacttc ttcaacaaca tgtgtgcaaa gaaaatcgtc gtcttaccag gagaccatat
      121 tggccctgaa attgttgctt ctgccttgga ggttttgaaa gtcgttgaga agaagcgacc
      181 tgagttaaaa ctcgagtttg aagaacacaa gattggaggt gcctctattg atgcctatgg
      241 aacccctttg actgacgaga ctgtgaaggc ttgtttggaa gctgacggtg ttcttttggg
      301 tgccgttggt ggtcctgaat ggaccaaccc caattgtcgt cctgagcaag gtttattgaa
      361 gcttcgtaag agtatgggtg tttgggccaa ccttcgacct tgcaactttg ccagcaagtc
      421 tttagtcaag tacagccctt tgaagcctga aatcgttgaa ggtgtcgatt tttgtgttgt
      481 acgagaactt actggaggtt gttactttgg tgagcgcact gaggacaacg gatcgggtta
      541 tgctatggac acttggcctt acagtttgga agaagtttct cgtattgctc gtttggctgc
      601 ttggttagct gaaacttcca accctcctgc tcccgtcaca ttactcgaca aagctaatgt
      661 tttggcaact tctcgtcttt ggcgcaagac tgtagctaag atttttaagg aggaatatcc
      721 tcaccttacc ttaaaaaacc agctcattga ctccgcagcc atgcttttgg tcaagagccc
      781 tcgtacactt aacggtgttg ttttgactga caacttgttt ggtgacatta tttcagatga
      841 ggcttctgtc attcctggta gcttgggcct tttgccttct gcctcccttt ccggtgtggt
      901 aggaaaatca gaagaaaagg ttcattgttt ggttgagccc attcacggta gcgctcccga
      961 tatcgctggc aagggcattg ttaatcctgt tggtacaatt ttatctgctt cccttctcct
     1021 tcgttatggt ttgaatgctc ctaaggaggc tgaagctatc gaagccgccg tacgcaaggt
     1081 cttggatgat acttcaattg gtggacgtgg tctttatact cgcgatttgg gaggtgaggc
     1141 ttctaccgct gatattacta aggctgttgt tgaagaactt gaaaaaattt tgtaataggg
     1201 tttaatgtag aataaattca tatgatttga tttgacctaa tttttattga ttaattgtgt
     1261 cgcatattta ttatcgttaa aaatgcaatt gacaaaagcg taaattttaa ggttctaatg
     1321 ttttttcttt tggtatgtta tagtcaagca aagatgtatt aagttgcaaa agcatggaat
     1381 tgatttacga catccgttca actttatgac cgagttgtcg ctgtgtactt actctttttt
     1441 tactatgaaa agtttcaaac ataatatcat taattatttc tttttaaacg aagtaacctg
     1501 taacataaat aataggggaa aagaagaaaa aaaccataaa aattcgagaa cgtaacaatt
     1561 attattgtaa cgatgacaac ccgattcttt gagagagcca gagacatgac ttttgcatta
     1621 ccgtaattgg tttctactta tgaattgtaa acttgtgtag ggaatttcat ccacagctat
     1681 cgcgggctgc tctctgcatc agtgttagca cattttatat taatgaagat tttgctatgc
     1741 aaaatacatt gacaaggagg agggcaccac acaaaaagtt aggtgtaaca gaaaatcatg
     1801 aaactatgat tcctaattta tatattggag gattttctct aaaaaaaaaa aaatacaaca
     1861 aataaaaaac actcaatgac ctgaccattt gatggagttt aagtcaatac cttcttgaac
     1921 catttcccat aatggtgaaa gttccctcaa gaattttact ctgtcagaaa cggccttaac
     1981 gacgtagtcg acaagcttgc atgccctaca acaactaaga aaatggctat catgcggaag
     2041 ctggtgagaa gaacagcatc gggacaaggg aaggaagaac aaagacaaag aaaacaaaag
     2101 aaagcaattg aaaacaaaac aaaacaattt tcattccttc tcttatcatt ccttttcttt
     2161 tcttttctct cattcaacgc actccatcgt atccgtattc ctcttatttt ttctctttct
     2221 ctatatccat ttctttctct ctaggtgtgt cctctctctc tcttcaattt ctctactccg
     2281 cattccaacg catccttccc ccaacctccc atttcctcct tacggcccga tagcgatcat
     2341 ctttccctcg ctatcactcg ctaccggccc ctcctctgca ccgtaacctc ctacgtattt
     2401 accatatcat aaagtttttt ccgacgctta tcgctgaccc cctgtcgccc tcctattggc
     2461 ttccggatta tcttcttgtc cataaggtga tccatgcttc ctgaagattc ccgaaatgtg
     2521 tccactttgg cggggaatca ttccatccac ttctttctct ctcgctttcc tcattcggcg
     2581 ctccccttcc gcgtctcatt ggtcttccgc tccgtttttg ctttgccgat gttacttggg
     2641 gagaggtgcg ataatccttt cgcaaaaact cggtttgacg cctcccatgg tataaatagt
     2701 gggtggtgga caggtgcctt cgcttttctt taagcaagag aattgctgca ggtcgactct
     2761 agaggatctc atatgggtac ccgcccatat gcttgccctg tcgagtcctg cgatcgccgc
     2821 ttttctcgct cggatgagct tacccgccat atccgcatcc ataccggtca gaagcccttc
     2881 cagtgtcgaa tctgcatgcg taacttcagt cgtagtgacc accttaccac ccacatccgc
     2941 acccacacag gcgagaagcc ttttgcctgt gacatttgtg ggaggaagtt tgccaggagt
     3001 gatgaacgca agaggcatac caaaatccat acaggtggcg gaggcacacc tgcagctgcg
     3061 tcgactctag aggatccatc tgctggagac atgagagctg ccaacctttg gccaagcccg
     3121 ctcatgatca aacgctctaa gaagaacagc ctggccttgt ccctgacggc cgaccagatg
     3181 gtcagtgcct tgttggatgc tgagcccccc atactctatt ccgagtatga tcctaccaga
     3241 cccttcagtg aagcttcgat gatgggctta ctgaccaacc tggcagacag ggagctggtt
     3301 cacatgatca actgggcgaa gagggtgcca ggctttgtgg atttgaccct ccatgatcag
     3361 gtccaccttc tagaatgtgc ctggctagag atcctgatga ttggtctcgt ctggcgctcc
     3421 atggagcacc cagtgaagct actgtttgct cctaacttgc tcttggacag gaaccaggga
     3481 aaatgtgtag agggcatggt ggagatcttc gacatgctgc tggctacatc atctcggttc
     3541 cgcatgatga atctgcaggg agaggagttt gtgtgcctca aatctattat tttgcttaat
     3601 tctggagtgt acacatttct gtccagcacc ctgaagtctc tggaagagaa ggaccatatc
     3661 caccgagtcc tggacaagat cacagacact ttgatccacc tgatggccaa ggcaggcctg
     3721 accctgcagc agcagcacca gcggctggcc cagctcctcc tcatcctctc ccacatcagg
     3781 cacatgagta acaaaggcat ggagcatctg tacagcatga agtgcaagaa cgtggtgccc
     3841 ctctatgacc tgctgctgga gatgctggac gcccaccgcc tacatgcgcc cactagccgt
     3901 ggaggggcat ccgtggagga gacggaccaa agccacttgg ccactgcggg ctctacttca
     3961 tcggagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     4021 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
     4081 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
     4141 gcccttggaa ttgacgagta cggtgggtag gagctccagc ttttgttccc tttagtgagg
     4201 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     4261 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4321 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4381 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4441 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4501 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4561 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4621 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4681 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4741 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4801 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4861 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4921 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4981 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5041 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5101 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5161 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5221 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5281 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5341 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5401 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5461 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5521 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5581 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5641 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5701 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5761 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5821 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5881 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5941 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6001 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6061 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6361 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6421 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     6481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6541 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     6601 ggcatcagag cagattgtac tgagagtgca ccatacgata tcccaatctg tagtataaaa
     6661 agtgcaacag tgttga
//
