LOCUS       sequence-182950-        9769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses eArchT 3.0-eYFP in Phox2 (including catecholamine) 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Burke PG, Kanbar R, Basting TM, Hodges WM, Viar KE, Stornetta RL, 
            Guyenet PG
  TITLE     State-dependent control of breathing by the retrotrapezoid nucleus.
  JOURNAL   J Physiol. 2015 Jul 1;593(13):2909-26. doi: 10.1113/JP270053. Epub 
            2015 May 22.
  PUBMED    25820491
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1113/JP270053"; 
            journalName: "J Physiol"; date: "2015-07-1- 1"; volume: "593"; 
            issue: "13"; pages: "2909-26"
COMMENT     Sequence Label: pLenti-PRSX8 eArchT3.0-EYFP
FEATURES             Location/Qualifiers
     source          1..9769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        123..502
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        504..702
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     657..677
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             720..900
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    947..1072
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1565..1798
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1983..2027
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2325..2442
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2762..2771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3581..3583,3584..4294)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3623..3644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3884..3903)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4231..4252
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4346..4934
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4399..4419)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4817..4828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4937..4953)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4938..4954)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5459..5639
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5665..5682)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5671..5895
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5941..6369
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6028..6047)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6238..6259
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6378..6398)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6383..6712
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6563..6698
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6625..6644
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6760..6807
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6826..7200
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7330..7451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7367..7386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7421..7440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7500..7516)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7500..7516)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7513..7535)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7524..7540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7548..7554,7555..7572,7573..7578))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7593..7614
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7731..7748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7902..8490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7982..8001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8661..9452,9453..9521))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9284..9303
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9522..9626)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9701..9720)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
       61 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      121 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      181 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      241 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      301 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      361 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      421 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      481 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      541 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      601 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      661 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
      721 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      781 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      841 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      901 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      961 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1021 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1081 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1141 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1201 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1261 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1321 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1381 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     1441 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1501 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1561 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1621 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1681 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1741 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1801 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1861 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1921 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1981 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2041 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2101 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2161 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2221 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2281 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2341 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2401 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2461 agcagagatc cagtttggtt aattaaacgc gtataagctt ccgctagaca aatgtgatta
     2521 cccccgctag acaaatgtga ttacccgcgc tagacaaatg tgattacccc gctagacaaa
     2581 tgtgattacc ccccgctaga caaatgtgat tacccccgct agacaaatgt gattacccgc
     2641 gctagacaaa tgtgattacc ccgctagaca aatgtgatta cccccgacca gggcataaat
     2701 ggccaggtgg gaccagagag ctcaccccag ccgactctag aggatccccg ggtaccggta
     2761 ggccaccatg gacccaattg cactgcaggc ggggtatgac ctcctcggtg acgggaggcc
     2821 cgagacactc tggctgggca tcgggacgct tctcatgctc atcggaacgt tttacttcat
     2881 cgtcaaagga tggggtgtaa cagacaagga agcgcgcgag tactactcga tcactattct
     2941 tgtgccgggc atcgcctcag cggcgtattt gagcatgttc tttggcatcg ggctgaccga
     3001 agtgacggta gcgggagagg tcttggatat ctactatgcg cgctacgcag actggctgtt
     3061 tacaactccg ctccttttgc ttgatcttgc tctgttggca aaagtagata gggtgtcgat
     3121 tgggacgctg gtgggagtag atgcccttat gattgtcacg ggattgattg gggcattgtc
     3181 gcacacgcct ctcgcccgat attcgtggtg gttgttttcc accatctgca tgatcgtggt
     3241 cctctacttc ttggcgacga gccttcgggc tgcggcgaag gagagaggac ccgaggtcgc
     3301 gtccacattc aacaccttga ctgccctggt gctggtgctc tggaccgcgt atcccatctt
     3361 gtggattatc ggaactgaag gtgccggggt cgtcggtctg ggaatcgaaa cactcctctt
     3421 catggtgttg gacgtaacag ccaaggtcgg atttggtttc atcttgctgc ggtcaagagc
     3481 aattcttggg gatacggaag ccccggagcc tgcggccgcc aagagcagga tcaccagcga
     3541 gggcgagtac atccccctgg accagatcga catcaacgtg gtgagcaagg gcgaggagct
     3601 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3661 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3721 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     3781 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3841 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3901 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3961 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     4021 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     4081 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4141 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     4201 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4261 cgggatcact ctcggcatgg acgagctgta caagttctgc tacgagaacg aggtgtaatg
     4321 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     4381 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4441 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4501 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4561 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4621 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4681 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4741 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4801 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4861 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4921 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     4981 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     5041 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     5101 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5161 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     5221 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     5281 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     5341 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     5401 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     5461 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5521 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5581 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5641 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5701 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5761 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5821 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     5881 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     5941 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6001 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6061 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6121 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6181 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6241 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6301 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6361 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6421 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6541 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6601 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     6661 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6721 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     6781 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     6841 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     6901 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     6961 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     7021 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     7081 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     7141 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     7201 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7261 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     7321 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7381 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7441 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     7501 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7561 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7621 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7681 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7741 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7801 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7861 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7921 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7981 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8041 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8101 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8161 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8221 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8281 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8341 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8401 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8461 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8521 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8581 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8641 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8701 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8761 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8821 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8881 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8941 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9001 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9061 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9121 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9181 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9241 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9301 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9361 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9421 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9481 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9541 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9601 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     9661 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     9721 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctga
//
