LOCUS       sequence-182951-        9859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ChR2 (H134R) -eYFP in Phox2 (including catecholamine) 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Holloway BB, Viar KE, Stornetta RL, Guyenet PG
  TITLE     The retrotrapezoid nucleus stimulates breathing by releasing 
            glutamate in adult conscious mice.
  JOURNAL   Eur J Neurosci. 2015 Sep;42(6):2271-82. doi: 10.1111/ejn.12996. Epub
            2015 Jul 23.
  PUBMED    26096172
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/ejn.12996";
            journalName: "Eur J Neurosci"; date: "2015-09"; volume: "42"; issue:
            "6"; pages: "2271-82"
COMMENT     Sequence Label: pLenti-PRSX8ChR2 (H134R)eYFP
FEATURES             Location/Qualifiers
     source          1..9859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(7..26)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        198..577
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        579..777
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     732..752
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             795..975
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1022..1147
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1640..1873
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2058..2102
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2400..2517
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2825..2834
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2831..3757
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(3770..3772,3773..4486)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3812..3833)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4073..4092)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4420..4441
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4511..5099
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4564..4584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4982..4993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5102..5118)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5103..5119)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5624..5804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5830..5847)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5836..6060
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6106..6534
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6193..6212)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6403..6424
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6543..6563)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6548..6877
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6728..6863
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6790..6809
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6925..6972
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6991..7365
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7495..7616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7532..7551)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7586..7605
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7665..7681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7665..7681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7678..7700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7689..7705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7713..7719,7720..7737,7738..7743))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7758..7779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7896..7913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8067..8655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8147..8166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8826..9617,9618..9686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9449..9468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9687..9791)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
       61 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
      121 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
      181 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
      241 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      301 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      361 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      421 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      481 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      541 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      601 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      661 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      721 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
      781 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      841 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      901 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      961 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     1021 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     1081 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     1141 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     1201 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     1261 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1321 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1381 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1441 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     1501 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     1561 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     1621 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     1681 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1741 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1801 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1861 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1921 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     1981 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     2041 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     2101 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     2161 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     2221 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     2281 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     2341 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     2401 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2461 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     2521 gtttattaca gggacagcag agatccagtt tggttaatta aaaacgcgta taagcttccg
     2581 ctagacaaat gtgattaccc ccgctagaca aatgtgatta cccgcgctag acaaatgtga
     2641 ttaccccgct agacaaatgt gattaccccc cgctagacaa atgtgattac ccccgctaga
     2701 caaatgtgat tacccgcgct agacaaatgt gattaccccg ctagacaaat gtgattaccc
     2761 ccgaccaggg cataaatggc caggtgggac cagagagctc accccagccg actctagaac
     2821 cggtgccacc atggactatg gcggcgcttt gtctgccgtc ggacgcgaac ttttgttcgt
     2881 tactaatcct gtggtggtga acgggtccgt cctggtccct gaggatcaat gttactgtgc
     2941 cggatggatt gaatctcgcg gcacgaacgg cgctcagacc gcgtcaaatg tcctgcagtg
     3001 gcttgcagca ggattcagca ttttgctgct gatgttctat gcctaccaaa cctggaaatc
     3061 tacatgcggc tgggaggaga tctatgtgtg cgccattgaa atggttaagg tgattctcga
     3121 gttctttttt gagtttaaga atccctctat gctctacctt gccacaggac accgggtgca
     3181 gtggctgcgc tatgcagagt ggctgctcac ttgtcctgtc atccttatcc gcctgagcaa
     3241 cctcaccggc ctgagcaacg actacagcag gagaaccatg ggactccttg tctcagacat
     3301 cgggactatc gtgtgggggg ctaccagcgc catggcaacc ggctatgtta aagtcatctt
     3361 cttttgtctt ggattgtgct atggcgcgaa cacatttttt cacgccgcca aagcatatat
     3421 cgagggttat catactgtgc caaagggtcg gtgccgccag gtcgtgaccg gcatggcatg
     3481 gctgtttttc gtgagctggg gtatgttccc aattctcttc attttggggc ccgaaggttt
     3541 tggcgtcctg agcgtctatg gctccaccgt aggtcacacg attattgatc tgatgagtaa
     3601 aaattgttgg gggttgttgg gacactacct gcgcgtcctg atccacgagc acatattgat
     3661 tcacggagat atccgcaaaa ccaccaaact gaacatcggc ggaacggaga tcgaggtcga
     3721 gactctcgtc gaagacgaag ccgaggccgg agccgtgcca gcggccgccg tgagcaaggg
     3781 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     3841 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3901 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     3961 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     4021 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     4081 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     4141 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     4201 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     4261 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     4321 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     4381 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     4441 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagaat tcgatatcaa
     4501 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4561 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4621 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4681 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4741 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4801 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4861 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4921 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4981 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5041 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     5101 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     5161 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     5221 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5281 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     5341 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     5401 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     5461 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     5521 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     5581 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     5641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     5821 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5881 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5941 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6001 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6061 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6121 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6181 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6241 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6301 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6361 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6421 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6481 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6541 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6601 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6661 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6721 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6781 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6841 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     6901 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     6961 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     7021 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     7081 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     7141 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     7201 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     7261 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     7321 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     7381 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7441 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7501 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7561 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7621 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7681 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7741 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7801 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7861 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7921 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7981 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8041 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8101 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8161 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8221 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8281 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8341 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8401 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8461 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8521 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8581 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8641 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8701 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8761 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8821 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8881 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8941 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9001 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9061 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9121 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9181 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9241 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9301 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9361 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9421 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9481 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9541 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9601 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9661 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9721 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9781 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     9841 ccgatcccct atggtgcac
//
