LOCUS       sequence-182987-       16353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16353)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 16353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG412
FEATURES             Location/Qualifiers
     source          1..16353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4782..4798)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5695..5715
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5728..5772
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        complement(8996..9014)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9035..9051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9035..9051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9048..9070)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9059..9075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9083..9089,9090..9107,9108..9113))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9128..9149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9266..9283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9437..10025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9517..9536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10196..10987,10988..11056))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10819..10838
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11057..11161)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11187..11642)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11324..11345)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11536..11555
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11769..11791
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11783..11800
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11784..11800
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11807..11826
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11807..11825
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     11851..11867
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(13824..13835)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cgccgatgga ataaaactaa taatttttct cgcaatttat agaaatcatg tgattttcct
       61 accgctaaat ttaaaatttc ctaatttttc actatatttc tcacaaaatg cattccgaaa
      121 actctcattt ttcagctttt caatacaaat cccaataaat gatttttcaa atataatgtt
      181 ctcaattgat atgttttatt tgaatgttgg aaaaaaccat aaaattaaaa aaaacttgaa
      241 ttattcaaaa ataattcggc ccttttacaa aaaattatca taaaaattaa atttggaaaa
      301 tctgtgattt gtcgctttgc cggaagtttc caattccggc aactaaccga tttgccgatt
      361 agctagaaat ttcaattccg acaatgtgcc gctttgccga tttgccggaa atttccaatt
      421 ccggcaatta acggacttgc cgattagcta gaaatttaac tttagataaa acttctcaac
      481 aaaatcaaat tggaaagtca gcgatttgcc gctttgccgg aagttttcag ttccggcatc
      541 taaccgattt gccgatttgc caaaaatgtc aattccgaaa aactgccgat ttgccgcttt
      601 gtcggaaatt tcaattccga caaattgccg atttgccgat ttgccgaaca ttccaattcc
      661 gaccatttgt cgatttgccg gaagttacca attccggcaa ctaacggatt tgccgatttg
      721 ccggaaaaaa attgtttgtc gccaaccctg atttaaatga ctaatcatgt tgcaaaagat
      781 aactgcttgc atcaaaaatc cgatcattgg cattcccaac atcttcttca tccaaaacac
      841 cacccttttc ttccactaat catgaattcc cgaccaattg ttatcagtag gaaaagcctc
      901 cttttatctc attatcagca attgccttct tcttcttctt ctttgaatac catttgaatg
      961 gagatatcat ttacataaat ctgccgccga gattgtgaag caggaggaaa agctgaagat
     1021 actgatatta tatgtatatt tcattattct ttttccacaa aattttccga catttaattc
     1081 cacttcactt tcatcgtctc tttttccccc attgtcgccc ccactcattt gtcttcttct
     1141 tcaaatacgg gtcaatcact ttccgttttt tattcatttt ttgttatgtt cggcttaaca
     1201 aatttgaagt attgttaaaa ttctctagag cggccgccac cgcggtggcg gccgcgcgga
     1261 agatctggaa gtaaaatatt gtaatatttt ccctaaattt cacttaaaaa taaggatttt
     1321 tgcaacgtaa aacctcaaaa attcgctgaa attgacgatt tttgttcaaa attttgaaaa
     1381 acagggttaa ttttagctga aaatcttcaa ttttgccaga aattggcttt ttttccgtcg
     1441 ccacacggag aacttgccaa aaatgaagac ttttagccaa tttagccctg aaaattcgag
     1501 ttttgggcga tttttggtct gagaagccgt tttcgcagta tattatattt tctcgttaaa
     1561 tttcatttta aaataaacat tttgaacgta taaccccaaa aattcgctgc gcaatggatg
     1621 atttttgtcc aaagttttga taattttagc taaaaacctc gaattttggc aatttttcgt
     1681 gccgcatcgg ccgaaagttg actttttttg gaattatcat aatttaatgc acattttggt
     1741 agtttatctc acataatctg tctgcaggcg ggaaaaattt gagataaact actaaaagat
     1801 gcaaaacaag gatgataatt ccagaaaatc aacttcggac cgtctcgaca cggaaaattg
     1861 agcgaaattg gagttttaaa atgatttagc cctgaaaatt cgagaaattt aggcggtttt
     1921 gggtctgaaa aggcgttttc gcagtatttt ctcgctaaat ttcatttatt atcacataat
     1981 atcagtactt aggcgaatat cttgtagaaa tgttggtttt gttcgataat tctctacttt
     2041 tagagtcgat tcacgagaaa atgttgcacc atcttacttt tttcccttgt aaaacataga
     2101 ttcttctgta caatgaaaga gaacacaatt tcttcgtgaa gcaactcaaa aagtagagaa
     2161 aattcgaaca aaacccaaca tttctacaag atattcgtct aaatagtgat ataatgtgtt
     2221 aataagcata ttttaaatga aatttagcga aaaaactgcg aaaatttgtc aaaaaagcga
     2281 atttccatcc gatcagcaga tctctaaaag ttacataaaa ttgaaagttt tgtggggaaa
     2341 ttgttttaaa acacgcctat ggtaatacga gaacataaaa ttctgagaat gcgtattaca
     2401 caacatattc gacgcgcaaa atataactat atctcgtagc gaaaactaca gtaatcctta
     2461 aatgactact gtagcgattg tgtcgattta cgggctcgat tctcggaaaa aaaaatcatt
     2521 tttcaaattt tgacagcgat attcaatttt tcttcgtttt ttcgtatttc tcagtcattt
     2581 ttgtgcttat ttttaatatt ttattcatta ataaattaat ttaattgaaa aacgtccaga
     2641 cgtattttta catttcccgt ctatttccgt aataggcggt taataacgta gaaatacgtc
     2701 tgtatgagtt tactcatcaa aatgacacga atattggacg tttttaaatc aaattaattt
     2761 attaataggt aaaatattaa aaaataaaca caaaaatgac aaaggaatag gaaaacacga
     2821 agagaaattg agtatcgctg tcaaaattcg aaaaacgtgg atttttccaa gaatcgagcc
     2881 cgtaaatcga cacattcgct acagtagtca tttaaggatt attgtagttt tcgctacgag
     2941 atactttgcg cgccaaatat gttgtgaaat acgcattctc agaattttat gttcccgtaa
     3001 taccacaatg accgaatatc ataataaaaa aattcaaaaa caatttctag attttatatg
     3061 attttttgaa aattgaaaaa atctcagttt tcacctaatt atatttgaat taccgccaat
     3121 tgaactcgtt cgttggagcg cgcttgcatt attttcatta attaatttta ttaattttca
     3181 ttatttcact gattttcttc atttttgagg ttttttttat cgggaaatga aagaaatata
     3241 caagaaaaat gcaaaatgtt tattaaaaac tgaaattagt atttctcgga gttttaggca
     3301 ttttcagtta ctttttaata aagattttgc atttttcttg tatatttctt tcattttccg
     3361 attaaaaaac cccaaaaatg aagaaaatca gtgaaataat gaaaattaat tcaaataact
     3421 aatgaaaata atgcaagcgc gctccaacca acgagttcaa ttggcggtaa ttcaaatata
     3481 attaggtgaa aactgagatt ttttcaattt tcaaaaaatc atataacatc tagacaattt
     3541 ttttgaattt ttttatcatg atattcggtc attgtgtccc cataggcatg ttttaaagca
     3601 atttccacct ttaaataaaa tcgagaaaaa atggcgtcaa aagacatatg taaaagctgt
     3661 ttctctttgt atattcacac agaatatagc ccgattttgg aggtgaaaaa ccggtttttt
     3721 tgtattcttt acgccacata aagtgatatg gaagagaaaa tcgacatatt tatgtacttg
     3781 gagtacgagt acattggaga aaaagggtga actactggaa aatctgggaa atttcagaaa
     3841 aaaattccga aaaatctttt ttgctgaaaa aaaaatttgt tcgaaatttg ggaaatactg
     3901 gaaaattttc atcaaaatta aagcgatttt taacgttttt gcttgggaga tactatttgt
     3961 aattttaatg tttggtgagg acaacagaaa tgaccttcag cgtacggaaa acgattaaaa
     4021 gacacatttg aatcgaaaat agcgtgaaaa acatgaaaat atcggaaaaa tagctttaaa
     4081 attggatttg aaagcaaaaa atgtatcaaa aatcgagaaa aatgcttgaa aaagttgaaa
     4141 aactcaaaca aaaaatcaaa aaaggttttt ttaaaaatct gtgagtatcg caacgaaagc
     4201 acaacttgag gcatgcgcct ttaaaccaac cgtaaccaaa ttcggaggca aaaaaagatt
     4261 tctcgctgtt ttttcagttt taaaatcact tttcacgttt cttttcgcag taattcgttt
     4321 gcaaaacatc tgaaaaaatt ttattttata agaaattata taaaaatcgc tataaattga
     4381 gtttttgccc ccaaattcag cacggagcac ttctcaatta aatataattt tattttttaa
     4441 cattgaaagc tttcataaaa ttgagctttg tttgtaaata atcagtgaaa aaacacagaa
     4501 atcttcaaaa aataaagaaa aagtttttaa aaatctgtga gtcccgcaac gaaaataaaa
     4561 ctttaggcat gcgcctttaa accaaccgta accaaattcg gcggcaaaaa tggatttctc
     4621 gccgtttttt cagttttaca atcacttttt aggtttcttt ttgcagtatt tcgtttccca
     4681 aacaattaaa aatcaaattt tcttttccag atggctcaaa caaagccgat tgccgagcaa
     4741 atgggatccc ccgggcagca ggaaatcgat atcaagctta tcgataccgt cgacctcgag
     4801 ggggcggtac cggtagaaaa aatgaacctt attaaggaag atatgagagt caaagttcat
     4861 atggaaggaa acgtcaacgg tcatgcattt gttattgaag gagaaggaaa aggaaagcca
     4921 tacgaaggaa ctcaaactgc aaacttgact gtcaaagaag gagcaccact accatttagt
     4981 tacgtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcaggatatt
     5041 ctaactactg ccgtccatta cggaaacaga gtttttacta aatacccaga agatattcct
     5101 gattacttca agcaatcgtt tccagaagga tactcgtggg aaagaactat gactttcgaa
     5161 gataaaggta tttgcactat tgtaagttta aacagttcgg tactaactaa ccatacatat
     5221 ttaaattttc agagaagtga tattagtcta gaaggtgatt gcttcttcca aaatgtcaga
     5281 tttaaaggaa ctaactttcc tcctaacgga ccagttatgc aaaagaagac tcttaagtgg
     5341 gaaccatcga ctgaaaaact acatgttaga gatggactac ttgttggagt aagtttaaac
     5401 ttggacttac taactaacgg attatattta aattttcaga acattaacat ggcactacta
     5461 ctagaaggtg gaggtcacta cctttgcgat tttaaaacta cttacaaagc aaagaaggtc
     5521 gtccaacttc cagatgcaca ctttgttgat cacagaattg aaatactagg aaacgattcg
     5581 gattacaaca aagttaagct atacgaacac gcagttgcaa gatacagtcc tctaccaagt
     5641 caagcatggt gcccggggga tcggtggagc tccaccggtg gcggccgctc gagagagaat
     5701 ctttattttc agggcgccgc caaattcaaa gaaaccgctg ctgctaaatt cgaacgccag
     5761 cacatggaca gcggaggtgg aggtactagt ggatcctaat tttgccgtat tttccatatt
     5821 ttgttttgta tatttatcca ctcaccccct ctctttgtcc tgtgaatgaa cttgtgccaa
     5881 aaaagccaaa aataatttac tttttttaaa gacatattaa aacttgaaaa cttggaacaa
     5941 tacggtgcga aagcgaaaca gagacagaaa aatgaacaaa gagatccgtg aaaagtgaac
     6001 gatgatttag gcttcttgtt gggcggcgtg gatgagagtg atgttgtctc ctttgagaag
     6061 aattctgccg atcttgttgc gtcccttcgt cttcatgttc acctcctcgg cttcgtcgaa
     6121 cacgacattc atgaactcgt cgaatccgat gatgtagccc tcgagacggt gggtgacatc
     6181 ctcgtacaac cagatttgga cccgagtgcg gttttggaga tatctgaaat ttgggaaatt
     6241 gttttctact gattagtgaa aaaatattac ctgaagatga ggttcacagg ctgaaccatc
     6301 actttgttga gctttctcgt cgacattttt gcgggatgat ctgtaaaaat caatatattt
     6361 tttgaaagac acaaattttt aagataaata tcaagaaaac tcgattttag cagctattta
     6421 agctcaaaaa ctacaattta tggctttaaa acataatttt tcacatttta cataaatttc
     6481 aacgaaaaaa aatgccaaaa aacttcaaat ttgagcagaa atcggctatt ttctcgacaa
     6541 aaataatttc gaaaattcgt tcaaaaaaga tgagtacgca agcaccgtga cgcacagttg
     6601 cggactttgt aaaccgtacc cctgcataaa aaggcggaat tttgaattta ataaaatgca
     6661 gtttagaaca aagaaaagca cggagagaat tgtgataact aattatttgt atttaatttt
     6721 aaatactaca taaaactgaa gattggaacg agttaaagct ggtatttgcg gtttatagcc
     6781 ccaaaagtta aggcttgtta ggcaattatc gataaaaaca acgagaaatt cgtgaaatcg
     6841 gcaaaaattt atagaaaacg tttgaattaa attaaattta cgcttttgcc gccaacacct
     6901 atcgagaccc tagttatagg ggcggagcgt catgggagcg cgcttgcaac tcgatttcaa
     6961 aaagtttcgc tgtttgtaca acattctcag ttgttcttaa tcagttaaat aagtttcaaa
     7021 attttcaaaa tcataaaaca cagttaatgc taaattaaag tctaaaaaat caggtgataa
     7081 ggggtttgga cgaccaaaaa gcgaccttcg ctcgaaaatt gataaactaa ctacactttt
     7141 tccggaaaaa tgatggaaaa atcctgattt tcttcgttga atcaattttt gtttcatttt
     7201 taacactttc acatgaaata tttcagttaa aatgctcaag ctaattgggt ttcttataat
     7261 tttgggtctc gcaacggccg acaagtgctc agattgtttg gctcagggga aacagtgagt
     7321 tggaagctta caattctgag aattcgtatt gcgcagctta tttgacgcgc aaaatatgaa
     7381 atatcacgta gcgaaaacta cagtaattct ttaaataaga ctactgtagt gcctatgtcg
     7441 atttactgcc tcaactttct taatcaagct cgtaaatcga caccagctct acagtagtca
     7501 tagttttcgc tacgagatat tttgcgcgtc aaatatggtg tgcaatacgc attttcagaa
     7561 ttttatgttc tcgtaatata ccttttttca gctggtgtgt tactgtaaat gcatgtggat
     7621 ttacgccatg tgtcactaca attacagtga gtttttttga agaaaaaaaa attacccatt
     7681 tgatcgccaa ttccattttt caaaaatcaa taaaaaaaaa aaatttcggc gatcagaaac
     7741 gttacagaaa gccctaaact gcccggaact accagataaa gcccacgctt acgatgattc
     7801 atttatgagg aataaggtat tgatcacaca ttctgctgcc tacaatatgg acccacaaaa
     7861 gtgttttacg taggttcacc ttctagataa accttaaaaa tcgatacttt cagtagtaaa
     7921 atgccttcga tgaaagtttc caaaacagtt aatgtcaatt gtgatagata tgggccaagt
     7981 gagttgtttt attagcccac ggatttctgg cttccctcat aaattgaaaa ggaagagatt
     8041 gccgaactag accattttgg ctcggcgcgt tgcgtgtcac gtcgcggctc gattgtaaaa
     8101 ctaaatgtat tggtccgtgt ggagtacacg actttccctt tccggcaggc gattgtcaat
     8161 ggagcgcgaa aaattcaatg aggaaggcca gaaccccgtg tctgtagttt tctcaaaaag
     8221 tggcaaaaac tcaataattg ccaatttttg actgccaata aaaatttcac taaatttatt
     8281 aaatttattt taagttttat tgaaattttt ttaaaatttc cccactggcg ctaaaaaatc
     8341 caattttcag aagcaatgtg cttcagcttc acggccatag atactgtcca aaaagtgatt
     8401 gcaatgacgt ttagagggac agaaggagca gctcaattgt ctgaagaaat tttggatttc
     8461 ttccagtaag ttcgggaaat ggcaatttaa agtttaattg gatttttgta ttttttcttc
     8521 tcacgactac agttttgcac atttttatgt tataaaaaac caaaaaaaaa tttttttgcc
     8581 ttttttctca gcttaccaca aaacaatttc gaccaaaaat aaattgaaaa aattaaaaaa
     8641 ttttttgcac ggttttcgga ctcaaaaaaa gctgaatgtt cctggaacta tttgtaaaat
     8701 tcaaaattca aaattttcac gaattctcta aaaggttcca gcaaattcca caactttctt
     8761 gaatttttca aaaaagttct tgagcattca acaattattt ttggaatttt ccaaaggtta
     8821 caggacgttc caatttttca aaaaaatttc tcgcaaaaaa tgttttctca gaaaatttga
     8881 atttccctcc acataaaaga ttgttctcaa aaaatttgca aacacacatt ttcagcggaa
     8941 aaaaggcgtt cttcgactct ggaagtgtat tcgaatatgg taccagcttt tgttcccttt
     9001 agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9061 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9121 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9241 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9301 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9361 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9421 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9481 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9541 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9601 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9661 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9721 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9781 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9841 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9901 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9961 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10021 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10081 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10141 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10201 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10261 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10321 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10381 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10441 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10501 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10561 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10621 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10681 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10741 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10801 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10861 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10921 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10981 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11041 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11101 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11161 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
    11221 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
    11281 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
    11341 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
    11401 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
    11461 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
    11521 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
    11581 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
    11641 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11701 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11761 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
    11821 atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttcagta
    11881 aaagaagtag aattttatag ttttttttct gtttgaaaaa ttctccccat caatgttctt
    11941 tcaaataaat acatcactaa tgcaaagtat tctataacct catatctaaa ttcttcaaaa
    12001 tcttaacata tcttatcatt gctttaagtc aaacgtaaca ttaaaaaaaa tgttttggaa
    12061 aatgtgtcaa gtctctcaaa attcagtttt ttaaaccact cctatagtcc tatagtccta
    12121 tagttaccca tgaaatcctt atatattact gtaaaatgtt tcaaaaacca ttggcaaatt
    12181 gccagaactg aaaatttccg gcaaattggg gaaccggcaa attgccaatt tgctgaattt
    12241 gccggaaaaa cggtaattgc cgaaagtttt tgacacgaaa atggcaaatt gtggttttaa
    12301 aatttttttt tttggaaatt tcagaatttc aattttaatc ggcaaaactg taggcatcct
    12361 aagaatgttc ctacatctat tttgaaaagt aagcgaattc tatgaaaatg tctaaagaaa
    12421 atggggaaac aatttcaaaa aggcacagtt tcaagtgttt ccgaattata ctaaatccct
    12481 ctaaaaactt ccggcaaatt gatatccgta aaagagcaaa tccgcatttt tgccgaaaat
    12541 taaaatttcc gacaaatcgg caaaccggca atttggcgaa atttgccgga aggcaattgc
    12601 cgcccacccc tgttccagag gttcaaactg gtagcaaagc tcaaaatttc tcaaattctc
    12661 caattttttt ttgaattttg gcagtgtacc aaaatgacat tcagtcatat tggtttatta
    12721 tagatttatt tagataaaat cctaaatgat tctaccttta aagatgccca ctttaaaagt
    12781 aatgactcaa acttcaaatt gctctaagat tctattgaat taccatcttt tcctctcatt
    12841 ttctctcact gtctatttca tcacaaattc atccctctct ccctctcttt ctctctccct
    12901 ctctctcttt ctctttgctc atcatctgtc attttgtccg ttcctctctc tgcgccctca
    12961 gcgttcccca cactctctcg cttctctttt cctagacgtc ttcttttttc atcttcttca
    13021 gccctttttc gccattttcc atctctgtca atcattacgg acgaccccca ttaatttttg
    13081 gatgtgctct gcgagcaaga gcacacggaa ctcatttatt ttttgttgga atttcttttt
    13141 cattttagtt agttaaaagg ttattcagtg caaaaattga ttttttgctc ttttggtaca
    13201 ttagaggggt ttgaaagttt tccataaaaa gggtttccgg agtaaaaaat ctaaaaattt
    13261 ttacaaacat ttccaaaaat tttgggaaca tatcttttca aaaactcaaa aaaaaacgca
    13321 aacatcataa atttaatcaa ttaattggat gcgttcaaat attttaaaaa tatttttgaa
    13381 ttgcatattt ctttactcat aaaaaaatta ttaatgttta agtaaaaaaa atatttcagg
    13441 attttaaaca aaaactgtaa atcccataaa acagtgttat tttttagtta tttttagtaa
    13501 ttatttgtga attaaaaaca catttttttt caaaatattc cttgaataaa taaaattgaa
    13561 agtgaattca aaatatttca ccaattttac aaaatttgaa aatttgaaaa aatattttct
    13621 accaaattgt gctcaaaaaa tattattaag ttcccaggaa atttatgatg aaaacttgga
    13681 atatttttgg taaaattttc tcaaaaaatt gaaagttgtc aaaaacacac taacactata
    13741 cctcgcggca tagaaaaaac tggtggccga attttttaaa ccaatttaaa ttaaatatat
    13801 ttttgcactt tgtacaaaaa aatttgaagt tcccaggaaa ttcatacgga aatattttcc
    13861 agaactttta taatttttat tttaattctc aaaatttaca ataaaaactt ggaaacctga
    13921 aatttgctta aagaataacc ctatccccca cggcctagaa aatactggtg gccgaatttt
    13981 ttccgcagcc acaccacctc taatctccag agcactctcc aaaattccca taatccccaa
    14041 aattttcagc tacaacagca tatatgaagg cagatctaca acaacaatcg atcgcacccg
    14101 gctcggcaac cttcccgtct caggtgagga agacgcagag aaatgagaga gagagcgtgt
    14161 ttatgcacgc agtatactaa ctcctgatat ccaatttcat atatcgatat gcttgctttc
    14221 ttttcttcta cgttgaaatt gtttgaactt ttctcaaaaa acgcggattt tagagagact
    14281 tagagttgct ccaaataaat ttaatttgca attttgcact tactattaga atctactttt
    14341 atagcgctaa aatgaccgaa taacacagta gattttaaaa gaaaatttta aaaattattt
    14401 ttttgtgcag tcaaatttcg ctagtttttg ccactcattt ttgaacattt gtatatgtgc
    14461 ctttcggtgg ttcaatcagg ggtggcacgt ggtgtcagag tgttccattt cggtttgatc
    14521 tacgttgatc tacaaaaaat gcgggagaag agacgcagag ttctcaactg atttcacatg
    14581 gttatgaacc aggggtgttc ggcaaatttg ccgtttgccg agctgggcaa attttgagat
    14641 ttgccgcaca ccccttttat gaacgtgctg acgtcacatc tttttggaca aagaattccc
    14701 gcattttttg tatatcaaac cgtgatggga cagcctggca ccacgtgggg ttggcggcaa
    14761 atgatttttt ccgacaaatc ggcaaattgg caaattgccg gaatttaaag tttctggcaa
    14821 attgttgatt tgccgaaaaa aaatttcaaa aaggcacagt tttaagtgtt tccgtcttat
    14881 aaaatatttc tctaaaaatt tccggaaaac ggaaaattgc cggaaataca aagtttcgtc
    14941 aaatcggcaa acctgcaatt tgccgaacgg caactgccgc caacacctgg ttcccaaaaa
    15001 agcgtgcact gtttgaatat taaaaaaaaa attcacacaa aaataataaa ttgaaatagg
    15061 ctaaattcac atctatgtac aaaaattgca tgccagcacc ggtcaatttt ctcaattact
    15121 ttttgtctat ttttgccgaa cataatttgc tgtttccttg gcagaataca gaaaactctt
    15181 ttttttttca gatgccccga cccccaccgg taaccgaaca ggctataacc accgaggcgg
    15241 agcttctcgc gaaaaatcaa attacaccaa atgatgtgct agctcttcca ggaatcactc
    15301 aaggtaagtg aatatttgct ctattgataa acgcgccgtg tactccacgt ggacaaacat
    15361 atcaaatttt caggattcct atgctcccca tcggcaaacg tctataacat tgagttcacc
    15421 aaattccaaa tccgtgacct cgacacggaa cacgtgctct tcgaaattgc aaaaccggaa
    15481 aatgagacgg aagagaatct gcaggcgcaa gccgaatcgg caagatatgt ccgatatcga
    15541 tttgcgccga attttctgaa attaaagacg gtcggcgcga cggtcgagtt caaggtcggc
    15601 gatgtgccga tcacacattt tcgaatgatt gaacggcact ttttcaagga tcgtttattg
    15661 aaatgctttg attttgaatt tggattctgt atgccaaatt cgagaaacaa ctgtgaacat
    15721 atctatgaat ttccacaact ttcacaacaa ctcagtgagt ttgaagcggg aaaaattatt
    15781 tgagctcatt ttgtcaatag agcgaatttt ctaggttttt aaaaataaaa taaatattga
    15841 caaaaagctg ccctacagta gttcaaaatg tgaaaacttg gaggaaattc gagttttctc
    15901 tgagaagttt ttggcgggta attcaaattt tctgagaaaa aaattagccg cgagaattca
    15961 aattttctga gaaaattatt tgacgggaaa ttcaaaattt ctggaaatgt attgaacatt
    16021 tgagaaattt tgttaaaaac tacattaaac cgataaaaaa gttcgcaaat tttttttaag
    16081 tgctcatggt gcatcgaaga gaagaattct tgccaaaact agcccatgaa attaaaaatt
    16141 atcgcggaaa aaaactgact aattggaaag taaaatgaaa tttgcaaaaa aaaaatatgt
    16201 ttttttgttg taaaattaaa aaaaaaactg tatattttca gtggacgaca tgatcaataa
    16261 tccaaacgag acccgctccg atagcttcta ttttgtggag aataagctcg taatgcacaa
    16321 taaggccgac tactcgtatg atgcataatt tcc
//
