LOCUS       sequence-183025-        8589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Y2H Gal4 DNA binding domain for Cus1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
  TITLE     SF3b1 mutations associated with myelodysplastic syndromes alter the 
            fidelity of branchsite selection in yeast.
  JOURNAL   Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 
            10.1093/nar/gkw1349.
  PUBMED    28062854
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349."
COMMENT     Sequence Label: pGBKT7-CUS1
FEATURES             Location/Qualifiers
     source          1..8589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..705
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             733..1173
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1184..1203
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1184..1202
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1222..1251
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      2595..2642
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2669..2856
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2724..2748)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2880..2896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2880..2896)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2893..2915)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2904..2920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2928..2934,2935..2952,2953..2958))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2973..2994
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3111..3128)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3282..3870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3362..3381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4199..4246
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4271..4290
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4478..5272)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4478..5272)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4589..4608)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5199..5218
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5273..5377)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5404..6746
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6367..6414)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6787..6805
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6843..6865)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6965..6984
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7005..7286
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7287..7961
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8064..8519)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8201..8222)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8413..8432
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca
       61 tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga
      121 gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt
      181 acactgcctc attgatggtg gtacataacg aactaatact gtagccctag acttgatagc
      241 catcatcata tcgaagtttc actacccttt ttccatttgc catctattga agtaataata
      301 ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca
      361 tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca
      421 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt
      481 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc
      541 cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac
      601 ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc
      661 tttttctgca caatatttca agctatacca agcatacaat caactccaag cttgaagcaa
      721 gcctcctgaa agatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt
      781 aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg
      841 gagtgtcgct actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa
      901 gtggaatcaa ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac
      961 cttgacatga ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta
     1021 tttgtacaag ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact
     1081 gatatgcctc taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt
     1141 agtaacaaag gtcaaagaca gttgactgta tcgccggaat ttgtaatacg actcactata
     1201 gggcgagccg ccatcatgga ggagcagaag ctgatctcag aggaggacct gcatatggct
     1261 agaaccaaaa gtaggaagcg ttccggaaat aaccaaaata agaatgcgtc tgtggtaaat
     1321 aataaggctg agatcgccgc catgattgat gcaagaaggc ttgagcaaaa gaaaaagggt
     1381 ggggtgacta acagcaaagg aaagacgaat aaggtagtgg atgcgaagtt agaaaaagag
     1441 ttcaaagacg ttttacaacg atttcaagtg caagagaatg atacgccgaa ggaaatcacg
     1501 aaagatgaaa agaacaatca tgtcgttatt gttgagaaaa acccagttat gaacagaaag
     1561 catacagcag aagatgaatt ggaggataca ccctcggatg gtattgagga gcatctatcg
     1621 gcaagaaaac gtaggaagac tgaaaaacct tcactttcgc agttaaaaag ccaggtaccg
     1681 tatcctcaaa ttatagaatg gtacgattgt gacgcaagat atccaggctt actagcgtca
     1741 attaagtgca ccaaaaatgt cattcctgtt ccaagccact ggcagtccaa gaaggaatat
     1801 ctatctggcc gttctctgtt aggtaaaaga ccttttgaac ttcctgacat tatcaagaag
     1861 acaaatatag aacaaatgag atcgacgctt ccgcaaagcg gactggatgg tcaagatgaa
     1921 aagtcactaa aggaggcctc aagagcaaga gtgcagccga aaatgggcgc cttggatttg
     1981 gattacaaga agttacacga tgtgtttttc aagataggag ccaactggaa acctgaccat
     2041 ttgttatgct tcggtgacgt ctattatgaa aataggaatc ttttcgagga aacgaactgg
     2101 aaaagaatgg ttgatcataa gagaccgggg agaattagcc aagagcttcg tgccataatg
     2161 aatttacccg aaggtcaact accaccatgg tgtatgaaga tgaaagacat tggattacct
     2221 acgggatatc ctgatctgaa aattgccggc ttgaattggg atataacgaa tttaaaaggc
     2281 gacgtttacg gaaaaataat ccctaatcac cattcaaggt ccaagaaaca gggtagaaac
     2341 tattttggcg cattgatctc atttgaaacc cctgaatttg aaaattcaaa agaggataca
     2401 caggcgaatg cggaaaatgg gcgccaagat gacaaaatcg atgatgaagt agagcataag
     2461 ttagatcact tccaggaaga catatccgaa gtgacaagcg cagaagaaaa acttgagaga
     2521 aacgaagagg aatcggagaa gcaactatat actgtattga aataaggatc cgtcgacctg
     2581 cagcggccgc ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     2641 tgcgcgcttg cagccaagct aattccgggc gaatttctta tgatttatga tttttattat
     2701 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
     2761 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
     2821 gcatgaggtc gctcttattg accacacctc taccggcatg caagcttggc gtaatcatgg
     2881 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2941 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3001 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3061 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3721 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4021 gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct
     4081 tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa
     4141 tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga
     4201 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
     4261 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac
     4321 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
     4381 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
     4441 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
     4501 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     4561 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     4621 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     4681 ccatgatatt cggcaagcag gcatcgccat gagtcacgac gagatcctcg ccgtcgggca
     4741 tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     4801 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     4861 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     4921 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     4981 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     5041 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat
     5101 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     5161 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     5221 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atactcttcc
     5281 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5341 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5401 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
     5461 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
     5521 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
     5581 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
     5641 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
     5701 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
     5761 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
     5821 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
     5881 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
     5941 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
     6001 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
     6061 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
     6121 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
     6181 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
     6241 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
     6301 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
     6361 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
     6421 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
     6481 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
     6541 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
     6601 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
     6661 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
     6721 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc
     6781 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     6841 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6901 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     6961 tatgcggcat cagagcagat tgtactgaga gtgcaccata gatcaacgac attactatat
     7021 atataatata ggaagcattt aatagaacag catcgtaata tatgtgtact ttgcagttat
     7081 gacgccagat ggcagtagtg gaagatattc tttattgaaa aatagcttgt caccttacgt
     7141 acaatcttga tccggagctt ttcttttttt gccgattaag aattaattcg gtcgaaaaaa
     7201 gaaaaggaga gggccaagag ggagggcatt ggtgactatt gagcacgtga gtatacgtga
     7261 ttaagcacac aaaggcagct tggagtatgt ctgttattaa tttcacaggt agttctggtc
     7321 cattggtgaa agtttgcggc ttgcagagca cagaggccgc agaatgtgct ctagattccg
     7381 atgctgactt gctgggtatt atatgtgtgc ccaatagaaa gagaacaatt gacccggtta
     7441 ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa tagttcaggc actccgaaat
     7501 acttggttgg cgtgtttcgt aatcaaccta aggaggatgt tttggctctg gtcaatgatt
     7561 acggcattga tatcgtccaa ctgcatggag atgagtcgtg gcaagaatac caagagttcc
     7621 tcggtttgcc agttattaaa agactcgtat ttccaaaaga ctgcaacata ctactcagtg
     7681 cagcttcaca gaaacctcat tcgtttattc ccttgtttga ttcagaagca ggtgggacag
     7741 gtgaactttt ggattggaac tcgatttctg actgggttgg aaggcaagag agccccgaaa
     7801 gcttacattt tatgttagct ggtggactga cgccagaaaa tgttggtgat gcgcttagat
     7861 taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga gacaaatggt gtaaaagact
     7921 ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata ggttattact gagtagtatt
     7981 tatttaagta ttgtttgtgc acttgccgat ctatgcggtg tgaaataccg cacagatgcg
     8041 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     8101 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     8161 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     8221 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     8281 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     8341 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     8401 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     8461 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     8521 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     8581 ttacgccag
//
