LOCUS       sequence-183033-        2432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMYA5 (NM_153610, nt 12205 to 12279) in pENTR3C, Gateway Entry 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Hsiung A, Naya FJ, Chen X, Shiang R
  TITLE     A schizophrenia associated CMYA5 allele displays differential 
            binding with desmin.
  JOURNAL   J Psychiatr Res. 2019 Apr;111:8-15. doi: 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10.
  PUBMED    30658136
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Psychiatr
            Res."; date: "2019-04"; volume: "111:8-15. doi"; pages: " 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10"
COMMENT     Sequence Label: CMYA5/pENTR3C
FEATURES             Location/Qualifiers
     source          1..2432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(59..145)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(310..898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(390..409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(988..1797)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1711..1730
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1789..1808
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1920..2019
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     2029..2045
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2030..2046
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2223..2322)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2341..2364)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(2372..2399)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
       61 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      121 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc ttagtagtta
      181 gacgtccccg agatccatgc tagcggtaat acggttatcc acagaatcag gggataacgc
      241 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      721 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      841 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacggggccc aatctgaata
      961 atgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg
     1021 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     1081 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     1141 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     1201 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     1261 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     1321 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     1381 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     1441 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg tttttccggg
     1501 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     1561 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     1621 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaagcg
     1681 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     1741 agcatccatg ttggaattta atcgcggcct cgacgtttcc cgttgaatat ggctcataac
     1801 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     1861 atcttgtgca atgtaacatc agagattttg agacacgggc cagagctgca gctggatggc
     1921 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat
     1981 gctttcttat aatgccaact ttgtacaaga aagctgggtc tagatatctc gaggtcgacg
     2041 gtatcgataa gcttgattga attccttgtg ccttacagaa tccgggggct ctatccccag
     2101 gtgcaaagta cattttccag gtttctccag ggcaaaggca gggtggacac cctcattaac
     2161 catggggatt ctgtaaggca caaggaattc aatcgaattc ctgcagcccg gttcctttaa
     2221 agagcctgct tttttgtaca aagttggcat tataaaaaag cattgcttat caatttgttg
     2281 caacgaacag gtcactatca gtcaaaataa aatcattatt tggggcccga gcttaagtaa
     2341 ctaactaaca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     2401 cttagtttga tgcctggcag tttatggcgg gc
//
