LOCUS       sequence-183044-        9886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label:  pLgw EcoDam-V5-ELK1
FEATURES             Location/Qualifiers
     source          1..9886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2367..2605
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2552..2573
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3500..3541
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3557..3581
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4899..4923)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             5454..5634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5660..5677)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5666..5890
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5936..6364
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6023..6042)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6233..6254
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6373..6393)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6378..6707
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6558..6693
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6620..6639
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6755..6802
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6821..7195
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7325..7446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7362..7381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7416..7435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7495..7511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7495..7511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7508..7530)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7519..7535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7543..7549,7550..7567,7568..7573))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7588..7609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7726..7743)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7897..8485)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7977..7996)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8656..9447,9448..9516))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9279..9298
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9517..9621)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9696..9715)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcccccggg
     2161 ctgcaggaat tcagatctcg gccgcatatt aagtgcattg ttctcgatac cgctaagtgc
     2221 attgttctcg ttagctcgat ggacaagtgc attgttctct tgctgaaagc tcgatggaca
     2281 agtgcattgt tctcttgctg aaagctcgat ggacaagtgc attgttctct tgctgaaagc
     2341 tcagtacccg ggagtaccct cgaccgccgg agtataaata gaggcgcttc gtctacggag
     2401 cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa
     2461 acaagcgcag ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat
     2521 taaaagtaac cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc aagaagtaat
     2581 tattgaatac aagaagagaa ctctgaatac tttcaacaag ttaccgagaa agaagaactc
     2641 acacacagct agcgccacca tgaagaaaaa tcgcgctttt ttgaagtggg cagggggcaa
     2701 gtatcccctg cttgatgata ttaaacggca tttgcccaag ggcgaatgtc tggttgagcc
     2761 ttttgtaggt gccgggtcgg tgtttctcaa caccgacttt tctcgttata tccttgccga
     2821 tatcaatagc gacctgatca gtctctataa cattgtgaag atgcgtactg atgagtacgt
     2881 acaggccgca cgcgagctgt ttgttcccga aacaaattgc gccgaggttt actatcagtt
     2941 ccgcgaagag ttcaacaaaa gccaggatcc gttccgtcgg gcggtactgt ttttatattt
     3001 gaaccgctac ggttacaacg gcctgtgtcg ttacaatctg cgcggtgagt ttaacgtgcc
     3061 gttcggccgc tacaaaaaac cctatttccc ggaagcagag ttgtatcact tcgctgaaaa
     3121 agcgcagaat gcctttttct attgtgagtc ttacgccgat agcatggcgc gcgcagatga
     3181 tgcatccgtc gtctattgcg atccgcctta tgcaccgctg tctgcgaccg ccaactttac
     3241 ggcgtatcac acaaacagtt ttacgcttga acaacaagcg catctggcgg agatcgccga
     3301 aggtctggtt gagcgccata ttccagtgct gatctccaat cacgatacga tgttaacgcg
     3361 tgagtggtat cagcgcgcaa aattgcatgt cgtcaaagtt cgacgcagta taagcagcaa
     3421 cggcggcaca cgtaaaaagg tggacgaact gctggctttg tacaaaccag gagtcgtttc
     3481 acccgcgaaa aaaaccggtg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     3541 gatcgatttc gatcaaacaa gtttgtacaa aaaagcaggc tccgaattcg cccttatgga
     3601 cccatctgtg acgctgtggc agtttctgct gcagcttctg agagaacaag gtaatggcca
     3661 catcatctcc tggacctcac gggatggtgg tgagttcaag ttggtggatg cagaggaggt
     3721 ggcccggcta tggggactgc gcaagaacaa gaccaacatg aattacgaca agcttagccg
     3781 ggccttgcgg tactactatg ataagaatat catccgcaag gtgagcggcc agaagtttgt
     3841 ctacaagttt gtgtcctacc cagaggttgc agggtgctcc actgaagact gcccacccca
     3901 gcctgaggtg tctgtaacct cggccatagc catggcccct gctactgtcc atgcaggccc
     3961 aggggacact gccactggaa agccaggaac accaaagggt gcaggaatga caggccaagg
     4021 tggcttagca cgaagcagcc ggaatgaata catgcgctcg ggcctctatt ctaccttcac
     4081 aatacaatcc ctgcagccac agccacagcc acccattcct cctcggcctg cctcagtgct
     4141 tcccaacact acccctgcag gagtaccagc acccgcctca gggagcagga gcaccagtcc
     4201 aaacccctta gaagcctgtt tggaagcaga agaggctggt ctgcccctgc aggttatcct
     4261 aaccccacca gaggccccaa accagaaatc cgaagagttg agtctggacc caagttttgg
     4321 ccatccacag cccccagaag tcaaagtgga ggggcctaag gaagaattgg aagctgcaag
     4381 ggctggaggc ttcagttcag aagctgtcaa agctgaacca gaagtctcag cctcagaagg
     4441 cctgctggct cggctcccag ccatcctaac agagaacaca gcccaggtgt gtggcctctc
     4501 cacttccacc actgagatca cccaaccgca gaagggccga aagcctcggg acctggaact
     4561 tccacttagc ccaagcctgc tgggtggcca gggacctgaa cggactccag gatcaggaac
     4621 aagctctggt cttcaggcac cggggccagc gctaacgcca tccctgctcc ccacacatac
     4681 cttgaccccg gtgctgctga cacccagctc gctgccccct agcatccatt tctggagcac
     4741 tctgagtcca attgcacccc gtagtccagc caagctctcc ttccagtttc cgtccagtgg
     4801 cagcgcacag gtgcacatcc cttccatcag tgtggatggc ctctcgaccc ccgtggtgct
     4861 ctccccaggg ccccagaagc catgaaaggg cgaattcgac ccagctttct tgtacaaagt
     4921 ggttcgatcg aaccgcggtc tagactcgag acctagaaaa acatggagca atcacaagta
     4981 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5041 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5101 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5161 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     5221 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     5281 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     5341 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     5401 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     5461 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5521 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5581 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     5641 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5701 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5761 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5821 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggta
     5881 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     5941 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6001 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6061 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6121 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6181 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6241 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6301 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6361 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6421 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6481 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6541 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6601 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6661 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     6721 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     6781 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     6841 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     6901 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     6961 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     7021 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     7081 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     7141 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     7201 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7261 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7321 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7381 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7441 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7501 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7561 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7621 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7681 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7741 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7801 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7861 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7921 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7981 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8041 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8101 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8161 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8221 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8281 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8341 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8401 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8461 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8521 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8581 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8641 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8701 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8761 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8821 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8881 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8941 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9001 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9061 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9121 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9181 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9241 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9301 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9361 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9421 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9481 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9541 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9601 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     9661 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9721 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     9781 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     9841 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
