LOCUS       sequence-183045-        7576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 Flag Jip1b
FEATURES             Location/Qualifiers
     source          1..7576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        121..500
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        501..704
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     655..675
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     749..768
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        749..767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             833..856
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(3015..3033)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3016..3033)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3053..3070)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3059..3283
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3329..3757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3416..3435)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3626..3647
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3766..3786)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3771..4100
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3951..4086
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4013..4032
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4167..4961
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4221..4240)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4831..4850
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5135..5256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5172..5191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5226..5245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5305..5321)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5305..5321)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5318..5340)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5329..5345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5353..5359,5360..5377,5378..5383))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5398..5419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5536..5553)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5707..6295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5787..5806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6466..7257,7258..7326))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7089..7108
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7327..7431)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7506..7525)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
       61 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      121 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      181 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      241 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      301 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      361 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      421 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      481 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      541 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      601 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      661 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      721 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      781 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattca tggattacaa
      841 ggatgacgac gataagatgg cggagcgaga gagcggcctg ggcgggggcg ccgcgtcccc
      901 accggccgct tccccattcc tgggactgca catcgcgtcg cctcccaatt tcaggctcac
      961 ccatgacatc agcctggagg agtttgagga tgaagacctt tcggagatca ctgacgagtg
     1021 tggcatcagc ctgcagtgca aagacaccct gtctctccgg cccccgcgcg ccgggctgct
     1081 gtctgcgggt agcagcggca gcgcggggag ccggctgcag gcggagatgc tgcagatgga
     1141 cctgatcgac gcggcaggtg acactccggg cgccgaggac gacgaggagg aggaggacga
     1201 cgagctcgct gcccaacgac caggagtggg gcctcccaaa gcggagtcca accaggatcc
     1261 ggcgcctcgc agccagggcc agggcccggg cacaggcagc ggagacacct accgacccaa
     1321 gaggcctacc acgctcaacc ttttcccgca ggtgccgcgg tctcaggaca cgctgaataa
     1381 taactcttta ggcaaaaagc acagttggca ggaccgtgtg tctcgatcat cctcccctct
     1441 gaagacagga gaacagacgc ctccacatga acacatctgc ctgagtgatg agctgccacc
     1501 ccagggcagt cctgttccca cccaggaccg cggcacttcc accgacagcc cttgtcgccg
     1561 aagtgcagcc acccagatgg cacctccaag cggtccccct gccactgcgc ctggtggccg
     1621 gggccactcc catcgagacc gaatccacta ccaggcagat gtgcggctcg aggcgactga
     1681 ggagatctac ctgaccccag tgcagaggcc cccagaccct gcagaaccca cctccacctt
     1741 catgccaccc acggagagcc ggatgtcagt tagctcggat ccagaccctg ccgcttactc
     1801 tgtaactgcg gggcggccac acccctccat cagtgaagag gatgagggct tcgactgcct
     1861 gtcatcccca gagcgagctg agccaccagg tggagggtgg cggggaagcc tcggggagcc
     1921 accaccgcct ccacgggcct cactgagctc ggacaccagc gcactgtcct acgactcggt
     1981 caagtacaca ctggtggtgg atgaacatgc ccagcttgag ttggtgagcc tgcggccgtg
     2041 ctttggagat tacagtgacg aaagcgactc tgccactgtc tatgacaact gtgcctctgc
     2101 ctcctcgccc tacgagtcag ccattggtga ggagtatgag gaggcccctc agccccggcc
     2161 tcccacctgc ctctcagagg actccacccc ggatgagcct gatgtccact tctctaagaa
     2221 gtttctgaat gtcttcatga gtggccgctc tcgttcctcc agtgctgagt cctttgggct
     2281 gttctcctgc gtcatcaatg gggaggagca tgagcaaacc catcgggcta tattcaggtt
     2341 tgtgcctcgg catgaagatg aacttgagct ggaagtggat gaccccctgc tggtggagct
     2401 gcaggcagaa gactattggt atgaggccta taacatgcgc accggagccc gcggggtctt
     2461 ccctgcctac tatgccattg aggtcaccaa ggagcctgag cacatggcag cccttgccaa
     2521 aaacagcgac tggattgacc agttccgggt gaagttcctg gggtctgtcc aggttcctta
     2581 tcacaagggc aatgatgtcc tctgtgctgc tatgcaaaag atcgccacca cccgccggct
     2641 caccgtgcac tttaacccgc cctccagctg tgtccttgag atcagtgtca ggggtgtcaa
     2701 gataggcgtc aaagctgatg atgctctgga ggccaaggga aataaatgta gccacttctt
     2761 ccagctaaag aacatctctt tctgtggata ccatccaaag aataacaagt actttgggtt
     2821 tatcactaag caccctgctg accaccggtt tgcctgccat gtctttgtgt ctgaagattc
     2881 caccaaagcc ctggcggagt ctgtggggcg tgcatttcag cagttctaca agcagtttgt
     2941 ggagtatacc tgtcctacag aagatatcta cttggagtag gcggccgctc gagcatgcat
     3001 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
     3061 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3121 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3181 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3241 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     3301 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     3361 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3421 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3481 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3541 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3601 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3661 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3721 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     3781 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3841 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3901 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3961 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4021 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4081 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     4141 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     4201 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4261 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4321 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     4381 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     4441 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     4501 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     4561 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     4621 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     4681 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     4741 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     4801 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     4861 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     4921 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     4981 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     5041 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     5101 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     5161 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5221 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     5281 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5341 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5401 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5461 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5521 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5581 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5641 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5701 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5761 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5821 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5881 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5941 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6001 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6061 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6121 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6181 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6241 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6301 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6361 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6421 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6481 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6541 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6601 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6661 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6721 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6781 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6841 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6901 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6961 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7021 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7081 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7141 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7201 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7261 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7321 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7381 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7441 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     7501 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     7561 ttggaggtcg ctgagt
//
