LOCUS       sequence-183059-        2847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of ChR2(H134R)-tdTomato under the Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Shepherd
  TITLE     Shepherd Lab _ Syn-ChR-tdTomato plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Syn-ChR-tdTomato
FEATURES             Location/Qualifiers
     source          1..2847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      419..428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             425..1351
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             1370..2797
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLGHGIGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVL
                     KGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYER
                     SEGRHHLFLYGMDELYK"
ORIGIN
        1 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
       61 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
      121 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
      181 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
      241 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
      301 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
      361 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga gaggatccgc
      421 caccatggac tatggcggcg ctttgtctgc cgtcggacgc gaacttttgt tcgttactaa
      481 tcctgtggtg gtgaacgggt ccgtcctggt ccctgaggat caatgttact gtgccggatg
      541 gattgaatct cgcggcacga acggcgctca gaccgcgtca aatgtcctgc agtggcttgc
      601 agcaggattc agcattttgc tgctgatgtt ctatgcctac caaacctgga aatctacatg
      661 cggctgggag gagatctatg tgtgcgccat tgaaatggtt aaggtgattc tcgagttctt
      721 ttttgagttt aagaatccct ctatgctcta ccttgccaca ggacaccggg tgcagtggct
      781 gcgctatgca gagtggctgc tcacttgtcc tgtcatcctt atcagactga gcaacctcac
      841 cggcctgagc aacgactaca gcaggagaac catgggactc cttgtctcag acatcgggac
      901 tatcgtgtgg ggggctacca gcgccatggc aaccggctat gttaaagtca tcttcttttg
      961 tcttggattg tgctatggcg cgaacacatt ttttcacgcc gccaaagcat atatcgaggg
     1021 ttatcatact gtgccaaagg gtcggtgccg ccaggtcgtg accggcatgg catggctgtt
     1081 tttcgtgagc tggggtatgt tcccaattct cttcattttg gggcccgaag gttttggcgt
     1141 cctgagcgtc tatggctcca ccgtaggtca cacgattatt gatctgatga gtaaaaattg
     1201 ttgggggttg ttgggacact acctgcgcgt cctgatccac gagcacatat tgattcacgg
     1261 agatatccgc aaaaccacca aactgaacat cggcggaacg gagatcgagg tcgagactct
     1321 cgtcgaagac gaagccgagg ccggagccgt ggcggcaccg gtagtagcag tgagcaaggg
     1381 cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
     1441 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     1501 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     1561 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
     1621 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
     1681 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
     1741 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga
     1801 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc
     1861 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa
     1921 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca
     1981 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt
     2041 cctggggcat ggcatcggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga
     2101 caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     2161 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2221 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     2281 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     2341 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2401 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     2461 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     2521 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     2581 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     2641 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     2701 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     2761 cctgttcctg tacggcatgg acgagctgta caagtaagaa ttccgtacaa agcttatcga
     2821 taatcaacct ctggattaca aaatttg
//
