LOCUS       sequence-183076-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses easily crystallizable engineered 14-3-3 sigma variant 
            fused to the HSPB6 phosphorylatable peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Sluchanko NN, Tugaeva KV, Greive SJ, Antson AA
  TITLE     Chimeric 14-3-3 proteins for unraveling interactions with 
            intrinsically disordered partners.
  JOURNAL   Sci Rep. 2017 Sep 20;7(1):12014. doi: 10.1038/s41598-017-12214-9.
  PUBMED    28931924
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-09-20"; volume: "7(1):12014. doi"; pages: " 
            10.1038/s41598-017-12214-9"
COMMENT     Sequence Label: pET28-his-3C_Clu3B6p
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..62
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             804..821
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaaggagata taccatggaa catcatcatc atcatcatct ggaggtgctg ttccaaggtc
       61 ctcatatgga gagagccagt ctgatccaga aggccaagct ggcagagcag gccgaacgct
      121 atgaggacat ggcagccttc atgaaaggcg ccgtggagaa gggcgaggag ctctcctgcg
      181 aagagcgaaa cctgctctca gtagcctata agaacgtggt gggcggccag agggctgcct
      241 ggagggtgct gtccagtatt gagcagaaaa gcaacgagga gggatcggca gcagcagggc
      301 ccgaggtgcg tgagtaccgg gagaaggtgg agactgagct ccagggcgtg tgcgacaccg
      361 tgctgggcct gctggacagc cacctcatca aggaggccgg ggacgccgag agccgggtct
      421 tctacctgaa gatgaagggt gactactacc gctacctggc cgaggtggcc accggtgacg
      481 acaagaagcg catcattgac tcagcccggt cagcctacca ggaggccatg gacatcagca
      541 agaaggagat gccgcccacc aaccccatcc gcctgggcct ggccctgaac ttttccgtct
      601 tccactacga gatcgccaac agccccgagg aggccatctc tctggccaag accactttcg
      661 acgaggccat ggctgatctg cacaccctca gcgaggactc ctacaaagac agcaccctca
      721 tcatgcagct gctgcgagac aacctgacac tgtggacggg atcgggatcc ctgcgccgcg
      781 ctagcgcccc gttgtgactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      841 aagcccgaaa
//
