LOCUS       sequence-183083-        8892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Y2H Gal4 DNA binding domain for Prp9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
  TITLE     SF3b1 mutations associated with myelodysplastic syndromes alter the 
            fidelity of branchsite selection in yeast.
  JOURNAL   Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 
            10.1093/nar/gkw1349.
  PUBMED    28062854
REFERENCE   2  (bases 1 to 8892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349."
COMMENT     Sequence Label: pGBKT7-PRP9
FEATURES             Location/Qualifiers
     source          1..8892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      2927..2974
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3001..3188
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3056..3080)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3212..3228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3212..3228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3225..3247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3236..3252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3260..3266,3267..3284,3285..3290))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3305..3326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3443..3460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3614..4202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3694..3713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4531..4578
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4603..4622
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4810..5604)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4810..5604)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4921..4940)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5531..5550
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5605..5709)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5736..7078
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6699..6746)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7119..7137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7175..7197)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7297..7316
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7337..7618
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7619..8293
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8396..8851)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8533..8554)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8745..8764
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcccgggt atgaatttac
     1321 ttgaaacaag gaggtccttg ttggaggaga tggagattat tgaaaatgcc atagcagaaa
     1381 gaattcagcg gaatccagag ttatattacc actatataca agaatcgagc aaggtgtttc
     1441 ctgatactaa actgcctaga tcatcgttga ttgcagagaa taaaatatac aagtttaaaa
     1501 aggttaagag gaagagaaaa cagataattt tgcagcaaca tgagataaat atttttcttc
     1561 gagactacca agagaaacaa caaactttta ataaaatcaa tcgtccagaa gagacacagg
     1621 aggatgacaa ggatttgcct aatttcgaaa gaaaactaca acagctagag aaggaactga
     1681 aaaatgaaga tgagaacttt gaattggata tcaactctaa aaaagacaaa tacgctttat
     1741 tctcatcttc ttctgatcca tcgaggcgca caaatatatt gtctgacaga gctcgagacc
     1801 tagacttaaa tgaaatattt actagagatg agcaatatgg tgaatatatg gagctggaac
     1861 aatttcattc tttatggttg aatgtaatta aacggggcga ttgttcactg cttcaatttc
     1921 tcgacatcct agaattattt ttggacgacg agaaatattt gctaacccca ccgatggatc
     1981 gcaagaatga tagatacatg gcctttttgc taaagttgag caaatatgta gaaacttttt
     2041 tcttcaaaag ttatgctttg cttgacgctg cggcagttga aaatctaatc aaatctgact
     2101 tcgaacattc atactgtagg ggatctcttc ggtccgaggc aaaaggtatc tattgccctt
     2161 tttgttcgag gtggttcaag acatcttccg ttttcgaaag ccatttagta gggaaaattc
     2221 ataagaaaaa tgaatctaaa agaagaaatt ttgtgtactc tgaatataaa ctgcatcggt
     2281 atttgaaata tttaaatgat gaattttctc gaacgagaag ttttgttgaa agaaaactgg
     2341 catttactgc aaatgaaaga atggcagaaa tggatatctt aacacagaag tatgaagcac
     2401 ctgcatatga ttcgacggaa aaagaggggg ccgaacaagt ggatggtgag cagagagatg
     2461 gtcaactgca agaagagcac ctctctggta aatcgtttga catgccattg ggtccggatg
     2521 gattgcctat gccatactgg ctatacaaac tgcatgggct tgacagagag tatcgctgcg
     2581 aaatttgttc gaataaagtt tataatgggc gacgcacttt tgaaagacat ttcaacgaag
     2641 aaagacatat ttatcacttg cgatgccttg gtatcgaacc ttcttcagta ttcaagggca
     2701 taaccaaaat taaggaggca caagagctct ggaaaaatat gcaggggcag tcacagttga
     2761 catctattgc agcagttccc ccaaagccta atccttcaca actaaaagtt cctacagaat
     2821 tagaactaga agaagaagac gaagaaggaa atgtaatgag taagaaggtc tacgatgaac
     2881 ttaagaagca aggtttggtg tgagtcgacc tgcagcggcc gcataactag cataacccct
     2941 tggggcctct aaacgggtct tgaggggttt tttgcgcgct tgcagccaag ctaattccgg
     3001 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta
     3061 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct
     3121 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc
     3181 tctaccggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3241 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3301 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3361 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3421 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3481 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3541 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3601 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3661 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3721 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3781 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3841 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3901 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3961 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4021 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4081 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4141 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4201 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4261 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4321 aatgaagttt taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg
     4381 ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg
     4441 aaaaggaaga aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt
     4501 gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat
     4561 tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt
     4621 cagttagcct ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga
     4681 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     4741 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca
     4801 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     4861 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     4921 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     4981 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     5041 atgagtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc
     5101 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     5161 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     5221 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     5281 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     5341 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     5401 cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac
     5461 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     5521 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     5581 gtgcaatcca tcttgttcaa tcatactctt cctttttcaa tattattgaa gcatttatca
     5641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5701 ggttccgcgc acatttcccc gaaaagtgcc acctgaacga agcatctgtg cttcattttg
     5761 tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     5821 ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc tgtgcttcat
     5881 ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     5941 catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa gaatctatac
     6001 ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca aagcatctta
     6061 gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac tttttgcact
     6121 gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt ccataaaaaa
     6181 agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg cattttttca
     6241 agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac tttgtgaaca
     6301 gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt ttcttctatt
     6361 ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt cgattcactc
     6421 tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga taaacataaa
     6481 aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg ggtaggttat
     6541 atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg tttgtggaag
     6601 cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt tttttgaaag
     6661 tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata ctttctagag
     6721 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
     6781 caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
     6841 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
     6901 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
     6961 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
     7021 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcat
     7081 actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     7141 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7201 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7261 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     7321 gagtgcacca tagatcaacg acattactat atatataata taggaagcat ttaatagaac
     7381 agcatcgtaa tatatgtgta ctttgcagtt atgacgccag atggcagtag tggaagatat
     7441 tctttattga aaaatagctt gtcaccttac gtacaatctt gatccggagc ttttcttttt
     7501 ttgccgatta agaattaatt cggtcgaaaa aagaaaagga gagggccaag agggagggca
     7561 ttggtgacta ttgagcacgt gagtatacgt gattaagcac acaaaggcag cttggagtat
     7621 gtctgttatt aatttcacag gtagttctgg tccattggtg aaagtttgcg gcttgcagag
     7681 cacagaggcc gcagaatgtg ctctagattc cgatgctgac ttgctgggta ttatatgtgt
     7741 gcccaataga aagagaacaa ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa
     7801 agcatataaa aatagttcag gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc
     7861 taaggaggat gttttggctc tggtcaatga ttacggcatt gatatcgtcc aactgcatgg
     7921 agatgagtcg tggcaagaat accaagagtt cctcggtttg ccagttatta aaagactcgt
     7981 atttccaaaa gactgcaaca tactactcag tgcagcttca cagaaacctc attcgtttat
     8041 tcccttgttt gattcagaag caggtgggac aggtgaactt ttggattgga actcgatttc
     8101 tgactgggtt ggaaggcaag agagccccga aagcttacat tttatgttag ctggtggact
     8161 gacgccagaa aatgttggtg atgcgcttag attaaatggc gttattggtg ttgatgtaag
     8221 cggaggtgtg gagacaaatg gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa
     8281 tgctaagaaa taggttatta ctgagtagta tttatttaag tattgtttgt gcacttgccg
     8341 atctatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     8401 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     8461 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     8521 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     8581 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     8641 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     8701 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     8761 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     8821 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     8881 gggaagggcg at
//
